What Is FRG2C and Why Does It Matter in Research?

FRG2C (FSHD Region Gene 2 Family Member C) belongs to the FRG2 gene family, which is located in chromosomal regions associated with facioscapulohumeral muscular dystrophy (FSHD) research. Members of this family have attracted scientific interest due to their proximity to repeat elements and their potential roles in gene regulation and chromatin organization.

Recombinant Human FRG2C Protein provides researchers with a well-defined, consistently produced protein source to investigate the molecular functions of this gene family member. Understanding FRG2C may contribute to broader insights into muscle development, epigenetic regulation, and disease mechanisms associated with the FSHD locus.

Note: Specific functional data for FRG2C remain an active area of investigation. Researchers are encouraged to consult current primary literature for the latest findings.

Storage, Handling, and Reconstitution Protocol

Proper handling of Recombinant Human FRG2C Protein is essential to maintain protein integrity and experimental reproducibility. Follow these general best-practice guidelines:

• Storage Temperature: Store lyophilized or liquid protein preparations at the temperature specified on the product datasheet (typically −20 °C or −80 °C for long-term storage). Avoid repeated freeze-thaw cycles, as these can cause protein aggregation and loss of activity.
• Reconstitution: Reconstitute lyophilized protein in the recommended volume of sterile, deionized water or the buffer specified in the product datasheet. Allow the vial to equilibrate to room temperature before reconstitution. Mix gently by pipetting; do not vortex.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw events and reduce the risk of contamination.
• Working Concentration: Determine the optimal working concentration empirically for each application. Refer to the product datasheet or application-specific literature for suggested starting concentrations.
• Carrier Protein: If long-term stability is a concern, consider adding a carrier protein (e.g., 0.1% BSA) to diluted aliquots, unless this would interfere with the intended assay.

Always consult the specific product datasheet (absea#PP-1860) for exact formulation, concentration, and storage conditions provided by the manufacturer.

Recommended Applications and Experimental Considerations

Recombinant Human FRG2C Protein is intended for research use and may be applicable in a range of in vitro and biochemical studies. Typical applications for recombinant proteins of this class include:

• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) to identify binding partners.
• Antibody Validation: Serve as a positive control antigen to verify the specificity and sensitivity of anti-FRG2C antibodies in Western blot, ELISA, or immunofluorescence assays.
• Structural Studies: Support X-ray crystallography, cryo-EM sample preparation, or NMR experiments when sufficient purity and homogeneity are confirmed.
• Functional Assays: Investigate potential enzymatic activity, DNA or RNA binding, or effects on chromatin-associated complexes in cell-free systems.
• Standard Curve Generation: Use as a quantification standard in sandwich ELISA or mass spectrometry-based absolute quantification workflows.

Validation of the protein in each specific application is strongly recommended prior to use in critical experiments. Lot-to-lot consistency should be verified if results must be compared across experiments performed with different lots.

Quality, Purity, and Regulatory Considerations

Research-grade recombinant proteins such as Recombinant Human FRG2C Protein are manufactured and quality-controlled to support consistent scientific results. Key quality considerations include:

• Purity Assessment: Purity is typically evaluated by SDS-PAGE and/or HPLC. The expected purity and the method used are stated in the product datasheet. Researchers should confirm purity meets the requirements of their specific application.
• Endotoxin Testing: For cell-based assays, endotoxin levels are a critical parameter. Verify endotoxin specifications on the datasheet (absea#PP-1860) before use in cell culture experiments.
• Biological Activity: Where applicable, biological activity may be confirmed using functional assays described in the datasheet. Activity can vary by lot; always document the lot number used in publications.
• For Research Use Only (RUO): This product is not approved for, and must not be used in, clinical diagnosis, therapeutic applications, or any regulated procedure requiring GMP-grade material.

Aladdin Scientific recommends retaining the original Certificate of Analysis (CoA) for each lot purchased, as this document contains lot-specific quality data essential for regulatory compliance and publication reproducibility.

Ordering, Procurement, and Technical Support

Recombinant Human FRG2C Protein is available from Aladdin Scientific under SKU rp366244. The following information is provided to assist procurement and laboratory management teams:

• How to Order: Place orders through the Aladdin Scientific website, by contacting your regional sales representative, or via your institution's approved procurement portal. Reference SKU rp366244 to ensure correct product selection.
• Pricing and Availability: Pricing may vary by quantity and region. Contact Aladdin Scientific sales for current pricing, volume discounts, and availability lead times.
• Shipping and Storage on Arrival: Products are shipped under conditions appropriate to maintain stability (e.g., dry ice for frozen products). Upon receipt, immediately transfer to the storage conditions specified on the datasheet to preserve product integrity.
• Returns and Refunds: If the product does not meet specifications, contact Aladdin Scientific customer service with the lot number and CoA. Refer to the company's returns and refunds policy for eligibility criteria.
• Technical Support: For application-specific questions, protocol optimization, or troubleshooting, contact the Aladdin Scientific technical support team. Please have the SKU and lot number available when reaching out.