What Is FRK and Why Is It Relevant to Research?

FRK (Fyn-related kinase), also known as RAK or BSK, is a non-receptor protein tyrosine kinase that is structurally related to the Src family of kinases. It contains an SH2 domain, an SH3 domain, and a catalytic kinase domain. Unlike most Src-family members, FRK has been reported to function as a negative regulator of cell proliferation and has been implicated in tumor suppression, particularly in breast cancer and glioblastoma models.

FRK phosphorylates substrates involved in cell-cycle regulation, including PTEN, thereby stabilizing PTEN activity and suppressing PI3K/AKT signaling. Because of its role at the intersection of growth-factor signaling and tumor suppression, FRK is a target of interest in oncology research, kinase inhibitor development, and signal-transduction studies.

Product Specifications and Quality Assurance

Recombinant Human FRK Protein (SKU: rp377166) is produced using a recombinant expression system and is intended for research applications. Key specification details, including exact molecular weight, purity percentage, endotoxin level, and formulation buffer, should be confirmed on the official product datasheet available from Aladdin Scientific, as these parameters may vary by lot.

Typically for recombinant kinase proteins of this class, researchers should expect: SDS-PAGE-verified purity, validation by activity assay or Western blot, and lot-specific quality control certificates. Please contact Aladdin Scientific technical support or consult the product datasheet (reference: absea#PP-11754) for confirmed specifications.

Recommended Applications and Experimental Use

Recombinant Human FRK Protein is suitable for a range of in vitro biochemical and cell biology research applications. Common uses for recombinant tyrosine kinase proteins such as FRK include:

• In vitro kinase activity assays: Measuring phosphorylation of peptide or protein substrates to characterize kinase activity or screen inhibitors.
• Protein–protein interaction studies: Using FRK as bait or prey in pull-down or co-immunoprecipitation experiments.
• Antibody validation: As a positive control antigen for Western blot or ELISA.
• Structural and biophysical studies: Including surface plasmon resonance (SPR) or isothermal titration calorimetry (ITC) for binding studies.

All applications should be validated by the end user under their specific experimental conditions. This product is for research use only and is not intended for clinical, diagnostic, or therapeutic purposes.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human FRK Protein are critical to maintaining activity and preventing degradation. General best-practice guidelines for recombinant kinase proteins include:

• Store at –80 °C for long-term storage upon receipt.
• Avoid repeated freeze–thaw cycles; aliquot into single-use volumes before freezing.
• Thaw on ice and keep on ice during use.
• Do not vortex; mix gently by pipetting.
• Centrifuge briefly before opening to collect contents at the bottom of the tube.

Specific storage buffer composition, recommended working concentrations, and stability data (shelf life) are provided on the product datasheet. Consult the datasheet for lot-specific information before use.

Ordering Information and Technical Support

Recombinant Human FRK Protein is available from Aladdin Scientific under SKU rp377166 (reference: absea#PP-11754). To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, application suitability, or troubleshooting, please reach out to the Aladdin Scientific technical support team. Certificate of Analysis (CoA) and Safety Data Sheet (SDS) documents are available upon request or via the product page on the Aladdin Scientific website.

Custom quantities, bulk pricing, or special formulations may be available; contact Aladdin Scientific for details.