What Is FRMD4A and Why Is It Relevant to Research?

FRMD4A (FERM Domain-Containing Protein 4A) is a member of the FERM superfamily of proteins, which are characterized by a conserved N-terminal FERM domain that mediates interactions with membrane lipids and cytoskeletal components. The FERM domain acts as a molecular scaffold, linking the plasma membrane to the actin cytoskeleton and facilitating the assembly of protein complexes critical for cell polarity establishment.

FRMD4A has been implicated in the regulation of epithelial cell polarity through its interaction with the Par3–aPKC polarity complex and the ARF guanine nucleotide exchange factor cytohesin. These interactions position FRMD4A as a coordinator of apical membrane identity and tight junction assembly in polarized epithelial cells. Dysregulation of FRMD4A has been associated with altered cell migration and changes in epithelial integrity, making it a protein of interest in cancer biology and developmental studies.

Recombinant Human FRMD4A Protein (rp372846) provides researchers with a well-defined reagent to study these molecular interactions in vitro, perform binding assays, generate antibody standards, or establish functional assays related to cell polarity signaling.

Product Specifications and Quality Information

Aladdin Scientific's Recombinant Human FRMD4A Protein (SKU: rp372846) is produced as a recombinant protein and is intended for research use only. Researchers should consult the product datasheet for confirmed details on the following parameters, as specific lot-level data supersedes general descriptions:

• Expression System: Please refer to the product datasheet for the confirmed expression host (e.g., E. coli, baculovirus/insect cells, or mammalian cells). The expression system affects post-translational modification status.
• Purity: Purity is determined by SDS-PAGE and/or other orthogonal methods. Specific purity percentage should be confirmed on the certificate of analysis (CoA) for each lot.
• Formulation and Buffer: Protein formulation details including buffer composition, pH, and carrier protein (if any) are provided on the datasheet.
• Molecular Weight: Apparent molecular weight on SDS-PAGE may differ from theoretical weight due to post-translational modifications or tag sequences. Confirm on the datasheet.
• Endotoxin Level: Endotoxin content is measured and should be confirmed on the CoA, particularly important for cell-based assays.

For the most current and lot-specific specifications, always download the datasheet and CoA from the Aladdin Scientific product page for rp372846.

Storage, Handling, and Stability Recommendations

Proper storage and handling of recombinant proteins are essential to maintain activity and avoid degradation. While specific conditions for Recombinant Human FRMD4A Protein must be confirmed on the product datasheet, the following general best practices apply to recombinant proteins of this class:

• Long-term storage: Store the protein at −80 °C upon receipt if it will not be used immediately. Avoid storing at −20 °C for extended periods as repeated freeze-thaw cycles accelerate denaturation.
• Working aliquots: Upon first thaw, prepare single-use aliquots to minimize freeze-thaw cycles. Repeated freeze-thaw cycles can significantly reduce protein activity and integrity.
• Short-term storage: If the protein will be used within days, it may be stored at 4 °C for a limited period as specified in the datasheet. Addition of a carrier protein such as 0.1% BSA may stabilize the protein in dilute solutions.
• Avoid: Exposure to repeated freeze-thaw cycles, prolonged incubation at room temperature, detergents incompatible with the formulation, and metal-catalyzed oxidation conditions.
• Reconstitution: If supplied lyophilized, reconstitute with the recommended volume of sterile water or buffer as indicated on the datasheet. Allow the vial to equilibrate and mix gently; do not vortex.

Always refer to the specific product datasheet for FRMD4A protein (rp372846) for validated storage conditions and shelf-life data.

Recommended Applications for Recombinant Human FRMD4A Protein

Recombinant Human FRMD4A Protein is designed for in vitro research applications. Researchers working in cell polarity, epithelial biology, and related signaling fields may find this protein useful in the following types of experiments:

• Protein–Protein Interaction Studies: FRMD4A interacts with components of the Par polarity complex and with cytohesins. Recombinant FRMD4A can be used in pull-down assays, co-immunoprecipitation validation, or surface plasmon resonance (SPR) experiments to characterize binding affinities and interaction domains.
• ELISA Standard and Antibody Validation: Recombinant protein can serve as a positive control or quantification standard in sandwich or indirect ELISA formats, and for validating anti-FRMD4A antibodies by Western blot or dot blot.
• Structural and Biochemical Assays: Suitable for biochemical characterization studies, including thermal shift assays to assess protein stability in different buffer conditions or in the presence of binding partners.
• Functional Assays: Where activity data are available, recombinant FRMD4A may be incorporated into reconstituted polarity complex assays to study downstream signaling events.
• Immunogen for Antibody Production: Highly pure recombinant protein can be used as an immunogen for generating research-grade custom antibodies against FRMD4A.

Suitability for specific applications should be confirmed on the datasheet. Not all applications may have been validated by Aladdin Scientific for this specific product lot.

Ordering, Shipping, and Technical Support

Recombinant Human FRMD4A Protein is available from Aladdin Scientific under SKU rp372846. The following information is relevant for procurement and logistics:

• How to Order: The product can be ordered directly through the Aladdin Scientific website by searching for SKU rp372846, or by contacting your regional Aladdin Scientific sales representative. For bulk or custom quantity inquiries, please contact the sales team directly.
• Shipping Conditions: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Please ensure that your facility is equipped to receive dry-ice shipments and that the protein is transferred to appropriate storage immediately upon receipt.
• Lead Time: Standard lead times apply. For time-sensitive projects, contact Aladdin Scientific in advance to confirm stock availability and estimated delivery dates.
• Returns and Replacements: If the product arrives damaged or does not meet the specifications stated on the CoA, contact Aladdin Scientific customer service promptly with your order number and a description of the issue. Refer to Aladdin Scientific's return and refund policy for eligibility criteria.
• Technical Support: For application-specific questions, protocol guidance, or troubleshooting assistance regarding Recombinant Human FRMD4A Protein, please contact the Aladdin Scientific technical support team via the website or email.