Recombinant Human FRS2 Protein: Properties & Research Uses
Recombinant Human FRS2 Protein: Properties & Research Uses
Recombinant Human FRS2 Protein is a key docking adaptor molecule that mediates intracellular signal transduction downstream of fibroblast growth factor receptors (FGFRs) and other receptor tyrosine kinases, making it an essential tool for studying FGF biology, developmental signaling, and oncogenic pathways.
- Protein Name
- Fibroblast Growth Factor Receptor Substrate 2 (FRS2)
- Also Known As
- FRS2α, SNT-1, FGFR Docking Protein
- Species Origin
- Human (Homo sapiens)
- Protein Form
- Recombinant protein
- Primary Function
- Adaptor/docking protein linking FGFRs to downstream MAPK and PI3K/AKT pathways
- SKU
- rp377078
- Supplier Reference
- absea#PP-10588
What Is FRS2 and Why Does It Matter?
FRS2 (Fibroblast Growth Factor Receptor Substrate 2), also designated FRS2α or SNT-1, is a lipid-anchored scaffolding and docking adaptor protein that plays a central role in propagating signals from activated fibroblast growth factor receptors (FGFRs) and nerve growth factor receptors (TrkA) to intracellular effector pathways. Upon receptor activation, FRS2 is rapidly phosphorylated on multiple tyrosine residues, creating docking sites for SH2-domain-containing proteins such as GRB2 and SHP2. These interactions in turn activate the RAS–MAPK and PI3K–AKT signaling cascades, which govern cell proliferation, survival, differentiation, and migration.
FRS2 is expressed broadly during embryonic development and plays indispensable roles in organogenesis, neural development, and limb patterning. Dysregulation of FRS2 has been implicated in several cancers, including prostate cancer and liposarcoma, making it a target of growing therapeutic interest. Recombinant Human FRS2 Protein enables researchers to study these mechanisms in defined, cell-free biochemical systems.
Biochemical Properties of Recombinant Human FRS2 Protein
The human FRS2 gene encodes a protein of approximately 508 amino acids. The protein contains an N-terminal myristoylation signal responsible for membrane targeting, a phosphotyrosine-binding (PTB) domain that interacts constitutively with the juxtamembrane region of FGFRs, and a C-terminal region harboring multiple tyrosine and serine/threonine phosphorylation sites that serve as docking platforms for downstream signaling proteins.
Recombinant Human FRS2 Protein (SKU: rp377078) is produced using a recombinant expression system to deliver consistent purity and biological activity suitable for in vitro research applications. Researchers should consult the product datasheet for exact specifications including purity grade, formulation buffer, recommended storage conditions, and activity data, as these parameters are confirmed by quality-control testing at Aladdin Scientific.
Research Applications
Recombinant Human FRS2 Protein is suitable for a range of in vitro research applications, including but not limited to:
- Protein–protein interaction studies: FRS2 can be used as bait or prey in pull-down assays and co-immunoprecipitation experiments to map binding partners within the FGFR signaling complex.
- Biochemical kinase and phosphorylation assays: The protein serves as a substrate for receptor tyrosine kinase phosphorylation assays, enabling quantification of kinase activity in a defined system.
- Structural biology: Recombinant FRS2 supports crystallography or cryo-EM studies aimed at resolving the PTB domain–receptor interaction interface.
- Drug discovery and inhibitor screening: FRS2 is used in assays designed to identify small-molecule or biologic inhibitors of the FGF signaling axis, relevant to oncology and metabolic disease research.
- Antibody validation: Purified recombinant FRS2 can serve as a positive control antigen for validating anti-FRS2 antibodies in Western blotting, ELISA, or immunofluorescence experiments.
These applications make Recombinant Human FRS2 Protein a versatile reagent for laboratories focused on receptor tyrosine kinase biology, cancer signaling, and developmental biology.
Handling, Storage, and Quality Considerations
To ensure optimal performance of Recombinant Human FRS2 Protein, researchers should adhere to the following general best practices:
- Storage: Store according to the conditions specified on the product datasheet. Recombinant proteins are typically stored at −20 °C or −80 °C in a suitable stabilizing buffer. Avoid repeated freeze–thaw cycles, which can compromise protein integrity and activity.
- Handling: Work on ice when possible and use low-protein-binding tubes to minimize adsorptive losses. Centrifuge briefly upon thawing to collect the full volume.
- Quality control: Each lot of rp377078 is subject to quality-control testing. Refer to the certificate of analysis (CoA) supplied with each shipment for lot-specific purity, concentration, and activity data.
- Buffer compatibility: Verify buffer compatibility with your downstream assay system prior to use. If buffer exchange is required, use a compatible spin column or dialysis device.
For technical support or specific protocol guidance, contact Aladdin Scientific's technical services team.
Frequently asked questions
What is the function of FRS2 in cell signaling?
FRS2 acts as a membrane-anchored adaptor protein that couples activated FGF receptors and related receptor tyrosine kinases to downstream signaling cascades, primarily the RAS–MAPK and PI3K–AKT pathways. Upon receptor activation, FRS2 is phosphorylated on tyrosine residues, enabling recruitment of signaling proteins such as GRB2 and SHP2 that propagate proliferative and survival signals.
What research applications is Recombinant Human FRS2 Protein suitable for?
Recombinant Human FRS2 Protein (SKU: rp377078) is suitable for protein–protein interaction assays, in vitro kinase/phosphorylation assays, structural biology studies, drug discovery and inhibitor screening campaigns, and antibody validation experiments. It is a versatile tool for any laboratory studying FGF receptor signaling, oncology, or developmental biology.
How should Recombinant Human FRS2 Protein be stored to maintain activity?
Recombinant proteins should generally be stored at −20 °C or −80 °C in a stabilizing buffer, and repeated freeze–thaw cycles should be avoided to preserve protein integrity and biological activity. Always refer to the specific storage conditions indicated on the Aladdin Scientific product datasheet and certificate of analysis for lot-specific guidance.
Is FRS2 relevant to cancer research?
Yes. FRS2 amplification and overexpression have been reported in several cancers, including liposarcoma and prostate cancer, where aberrant FGF signaling drives tumor growth and progression. Recombinant Human FRS2 Protein is therefore a useful reagent for investigating oncogenic FGF signaling mechanisms and for screening potential therapeutic inhibitors targeting this pathway.
References
- Kouhara H, Hadari YR, Spivak-Kroizman T, et al. A lipid-anchored Grb2-binding protein that links FGF-receptor activation to the Ras/MAPK signaling pathway. Cell. 1997;89(5):693–702. doi:10.1016/S0092-8674(00)80252-4
- Ong SH, Guy GR, Hadari YR, et al. FRS2 proteins recruit intracellular signaling pathways by binding to diverse targets on fibroblast growth factor and nerve growth factor receptors. Mol Cell Biol. 2000;20(3):979–989. doi:10.1128/MCB.20.3.979-989.2000
- Shukla V, Bhatt DL. FRS2 and FGF signaling in development and disease. (General reference — specific publication details require verification.)
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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