What Is GAB1 and Why Is It Important in Cell Signaling?

GAB1 (GRB2-associated binding protein 1) is a multi-site docking/scaffold protein that serves as a central hub in signal transduction networks activated by growth factors and cytokines. Upon stimulation of receptor tyrosine kinases (RTKs) such as EGFR, MET (hepatocyte growth factor receptor), FGFR, and insulin receptor, GAB1 becomes tyrosine-phosphorylated and recruits a broad array of downstream signaling molecules including the p85 subunit of PI3-kinase, the phosphatase SHP2 (PTPN11), GRB2, and PLC-γ.

Through these interactions, GAB1 amplifies and diversifies intracellular signals, influencing cell proliferation, survival, migration, and differentiation. GAB1 is also implicated in oncogenic signaling, as aberrant GAB1 activity has been linked to cancer progression in various tumour types. Studying GAB1 protein interactions and phosphorylation events is therefore of significant interest in both basic cell biology and translational cancer research.

Product Specifications and Quality Standards

Recombinant Human GAB1 Protein (SKU: rp365268) is produced using a recombinant expression system to ensure consistent batch-to-batch quality. Researchers should consult the official product datasheet for confirmed details on the following parameters, as specifications may be updated:

• Host expression system: Confirm on current datasheet (commonly E. coli, baculovirus/insect cell, or mammalian cell systems are used for scaffold proteins of this type)
• Purity: Typically ≥85% as assessed by SDS-PAGE; exact figure on datasheet
• Tag information: Fusion or affinity tags (e.g., His-tag, GST) used for purification — confirm on datasheet
• Formulation/buffer: Supplied in a defined buffer system; confirm composition and any carrier proteins on datasheet
• Endotoxin level: Tested to ensure suitability for cell-based assays; exact limit on datasheet

All specifications should be verified against the current product datasheet provided with each lot.

Handling, Storage, and Reconstitution Guidelines

Proper handling of Recombinant Human GAB1 Protein is essential to preserve its biological activity and structural integrity. The following general guidelines apply to recombinant proteins of this class; always confirm with the product-specific datasheet:

• Upon receipt: Briefly centrifuge vials before opening to collect contents. Inspect for clarity and absence of visible particulates.
• Short-term storage: Store at 4°C if the protein will be used within 1–2 weeks.
• Long-term storage: Store at -20°C or preferably -80°C for extended stability. Aliquot the protein into single-use volumes before freezing to avoid repeated freeze-thaw cycles, which can significantly reduce activity.
• Reconstitution: If supplied lyophilised, reconstitute in the buffer specified on the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex vigorously.
• Carrier protein: Addition of a carrier protein (e.g., 0.1% BSA) may improve stability during long-term storage — check datasheet recommendation.

Note: Specific storage temperatures, buffer compositions, and reconstitution volumes must be confirmed from the current lot datasheet.

Research Applications of Recombinant Human GAB1 Protein

Recombinant Human GAB1 Protein is a versatile research tool applicable across multiple experimental platforms:

• Protein-protein interaction studies: GAB1 serves as bait or prey in pull-down assays, co-immunoprecipitation experiments, and surface plasmon resonance (SPR) or bio-layer interferometry (BLI) binding studies to characterise interactions with SH2-domain-containing proteins such as GRB2, PI3K p85, and SHP2.
• Phosphorylation and kinase assays: Recombinant GAB1 can be used as a substrate to characterise kinase activity and to map tyrosine phosphorylation sites relevant to RTK signaling.
• Structural biology: Suitable as antigen for antibody generation and as standard in Western blot and ELISA assay development.
• Cell-based signaling research: Used to reconstitute or perturb GAB1-dependent pathways in cell-free or cell-based systems, aiding investigation of PI3K/AKT and RAS/MAPK pathway dynamics.
• Drug discovery: GAB1 protein can be used in screening assays to identify small molecules or biologics that disrupt oncogenic GAB1–effector interactions.

Ordering, Shipping, and Support

Recombinant Human GAB1 Protein (SKU: rp365268) is available to order directly from Aladdin Scientific. The following information is relevant for procurement and logistics:

• Ordering: Place orders through the Aladdin Scientific website or contact your regional sales representative. Reference SKU rp365268 when ordering.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Confirm shipping conditions with Aladdin Scientific at the time of order.
• Returns and quality concerns: If you receive a product that does not meet specifications, contact Aladdin Scientific customer service promptly. Refer to the returns and refunds policy on the Aladdin Scientific website.
• Technical support: For application-specific guidance, datasheet clarification, or troubleshooting, contact the Aladdin Scientific technical support team via the website or phone.
• Documentation: Certificate of Analysis (CoA), Safety Data Sheet (SDS), and product datasheet are available upon request or via the product page on the Aladdin Scientific website.