What Is GAB2 and Why Is It Biologically Important?

GAB2 (GRB2-Associated Binding protein 2) is a member of the DOS/Daughter of Sevenless family of scaffolding/adaptor proteins. It serves as a docking platform downstream of multiple receptor tyrosine kinases (RTKs), cytokine receptors, and immune receptors. Upon receptor activation, GAB2 is recruited to the plasma membrane where it becomes tyrosine-phosphorylated, enabling binding of signaling effectors including the p85 regulatory subunit of PI3-kinase and the phosphatase SHP2.

Through these interactions, GAB2 amplifies downstream Akt/PKB and ERK/MAPK pathways, influencing critical cellular processes such as proliferation, survival, differentiation, and migration. GAB2 is particularly important in hematopoietic cell signaling and has been identified as an oncogene amplified in certain breast cancers and leukemias, making it a target of significant research interest.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human GAB2 Protein (SKU: rp377043) is produced as a recombinant protein intended for in vitro research applications. Researchers should consult the product datasheet for confirmed details on the following attributes:

• Expression System: Verify on datasheet (commonly E. coli, insect, or mammalian cell expression for adaptor proteins of this class).
• Purity: Typically >90% as assessed by SDS-PAGE; confirm exact specification on product datasheet.
• Formulation: Provided in a stabilizing buffer; consult datasheet for exact composition.
• Molecular Weight: The human GAB2 protein has a predicted molecular weight of approximately 76 kDa, though apparent MW on SDS-PAGE may differ due to phosphorylation and sequence composition; confirm with datasheet.
• Endotoxin Level: Consult product datasheet for lot-specific endotoxin values.

All specifications are subject to lot-to-lot variation; always refer to the certificate of analysis (CoA) supplied with each lot.

Storage, Reconstitution, and Handling Best Practices

Proper handling of recombinant proteins is critical to maintaining activity and experimental reproducibility. Follow these general best practices for Recombinant Human GAB2 Protein:

• Long-term Storage: Store at -80 °C. Avoid repeated freeze-thaw cycles, which can degrade protein integrity and reduce activity.
• Short-term Storage: If the protein will be used within 1–2 weeks, storage at 4 °C may be acceptable; consult the product datasheet for confirmation.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze-thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute in sterile deionized water or the buffer recommended on the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex vigorously.
• Carrier Protein: Addition of 0.1% BSA may help stabilize dilute protein solutions; verify compatibility with your assay.
• Working Concentration: Determine optimal working concentrations empirically for each application.

Recommended Research Applications

Recombinant Human GAB2 Protein is suited for a range of in vitro research applications, including but not limited to:

• Protein–Protein Interaction Studies: GAB2 serves as an ideal bait or prey protein for pull-down assays to characterize its interactions with PI3K p85, SHP2, GRB2, and other signaling partners.
• Biochemical Assays: Use as a substrate or binding partner in kinase assays, phosphorylation studies, and enzyme-linked immunosorbent assays (ELISA).
• Western Blot Standards: Recombinant GAB2 can serve as a positive control or molecular weight marker reference in immunoblotting experiments.
• Antibody Validation: Use as an antigen to validate anti-GAB2 antibodies for specificity and sensitivity.
• Structural Studies: Suitable for in vitro structural and biophysical characterization when purity and homogeneity requirements are met.

This product is intended for research use only and is not approved for diagnostic or therapeutic applications.

Ordering, Quality Assurance, and Support

Recombinant Human GAB2 Protein is available from Aladdin Scientific under SKU rp377043. Each lot is manufactured under controlled conditions and is accompanied by a Certificate of Analysis (CoA) detailing purity, concentration, and quality control test results.

Ordering: Place orders online through the Aladdin Scientific website or contact your regional sales representative. Bulk quantities and custom formulations may be available upon request.

Quality Assurance: Aladdin Scientific conducts multi-step quality control testing. If you experience any issues with product performance, contact our technical support team with your lot number and CoA for expedited resolution.

Returns and Replacements: Products damaged during shipping or with documented quality failures are eligible for replacement or refund per Aladdin Scientific's returns and refunds policy. Contact customer service within the stipulated time window after receipt.

Technical Support: Our scientific support team is available to assist with reconstitution protocols, assay optimization, and application-specific troubleshooting. Please have your SKU and lot number ready when contacting support.