What Is GBA3 and Why Is It Important in Research?

GBA3, also known as cytosolic beta-glucosidase or glucosylceramidase beta 3, is a member of the Glycoside Hydrolase Family 1 (GH1). Unlike the lysosomal glucocerebrosidase GBA1, GBA3 is localized in the cytosol and is capable of hydrolyzing a broad range of beta-glucosidic bonds. It plays a role in the biotransformation of dietary and xenobiotic glycosides, including flavonoid glucosides, and has been implicated in the regulation of intracellular glucosylceramide levels.

GBA3 is of interest to researchers studying glycoside metabolism, flavonoid bioavailability, and the broader landscape of cytosolic glycoside hydrolases. Understanding GBA3 activity may also contribute to research on metabolic pathways relevant to lipid storage disorders and nutritional biochemistry.

Product Specifications and Quality Information

Aladdin Scientific's Recombinant Human GBA3 Protein (SKU: rp377178) is produced using recombinant expression systems to ensure consistent activity and purity suitable for research applications. Researchers should consult the product datasheet for the most current information regarding host expression system, molecular weight, purity grade, endotoxin levels, and formulation buffer, as these specifications are subject to lot-specific variation.

When evaluating this protein for your application, key parameters to confirm include: (1) purity as determined by SDS-PAGE or HPLC, (2) biological activity confirmed by enzyme activity assay, (3) endotoxin content if the protein is intended for cell-based assays, and (4) formulation and recommended reconstitution conditions.

Recommended Applications for Recombinant Human GBA3 Protein

Recombinant Human GBA3 Protein is suitable for a variety of in vitro research applications, including:

• Enzyme Activity Assays: Measurement of cytosolic beta-glucosidase activity using fluorogenic or chromogenic substrates such as 4-methylumbelliferyl-beta-D-glucopyranoside (4-MUG).
• Substrate Specificity Studies: Characterization of the substrate scope of GBA3, including natural glucosides and synthetic analogs.
• Biochemical Pathway Research: Investigation of glycoside metabolism and flavonoid biotransformation pathways.
• Inhibitor Screening: Identification and characterization of small-molecule inhibitors of GBA3 enzyme activity.
• Structural and Biophysical Studies: Use as a reference or study target in crystallography, cryo-EM, or biophysical interaction assays.

Suitability for specific applications should be validated by the end user. Aladdin Scientific recommends consulting the product datasheet for application-specific guidance.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GBA3 Protein are critical to preserving enzymatic activity and preventing protein degradation. General best-practice guidelines for recombinant proteins of this class include:

• Storage Temperature: Store at -20°C for short-term use or at -80°C for long-term storage.
• Freeze-Thaw Cycles: Avoid repeated freeze-thaw cycles, which can reduce enzymatic activity. Aliquot the protein upon receipt to minimize freeze-thaw events.
• Carrier Protein: If provided lyophilized or at low concentration, reconstitution in a buffer containing a carrier protein such as BSA (0.1–0.5%) is recommended to improve stability.
• Working Conditions: Perform enzymatic assays under conditions appropriate for GH1 family enzymes; consult the datasheet for recommended pH and temperature ranges.

Always refer to the specific lot's Certificate of Analysis (CoA) and product datasheet for precise storage and reconstitution instructions.

Ordering and Technical Support

Recombinant Human GBA3 Protein is available from Aladdin Scientific under SKU rp377178. To place an order or request a quote, contact your local Aladdin Scientific representative or visit the product page on the Aladdin Scientific website.

For technical questions regarding experimental design, application suitability, or troubleshooting, Aladdin Scientific's technical support team is available to assist. When contacting support, please have the following information ready: product SKU, lot number (from the vial label or CoA), intended application, and a description of any issues encountered.

Bulk quantities, custom formulations, or OEM supply inquiries can be directed to our specialized sales team. We are committed to supporting your research with consistent, high-quality recombinant proteins.