What Is GBX1 and Why Is It Studied?

GBX1 (Gastrulation Brain Homeobox 1) is a member of the homeobox transcription factor superfamily. Homeobox proteins are characterized by a conserved DNA-binding domain called the homeodomain, which allows them to regulate gene expression during development and differentiation. GBX1 has been implicated in neuronal development, gastrulation, and patterning of the central nervous system. Research into GBX1 function may provide insights into developmental disorders, oncogenesis, and transcriptional regulatory networks. Recombinant Human GBX1 Protein enables direct biochemical characterization of this factor outside the cellular context.

Product Specifications and Quality

Recombinant Human GBX1 Protein (SKU: rp376869) is produced using recombinant expression technology to yield a protein suitable for research use. Key quality attributes typically assessed for recombinant proteins of this class include: (1) Purity — typically verified by SDS-PAGE and/or HPLC to confirm the absence of major contaminants; (2) Identity — confirmed by western blot or mass spectrometry against the expected molecular weight of the GBX1 polypeptide; (3) Biological activity — assessed where applicable via DNA-binding or transcription reporter assays. Please refer to the individual product datasheet for lot-specific purity and activity data, as specifications may vary between production lots.

Storage, Handling, and Reconstitution

Proper storage and handling are essential to maintaining the activity and integrity of recombinant proteins. The following general guidelines apply to Recombinant Human GBX1 Protein:

• Short-term storage: Store at -20°C for up to several weeks if the protein will be used frequently.
• Long-term storage: Store at -80°C for extended periods. Aliquot the protein upon first thaw to avoid repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
• Reconstitution: If supplied in lyophilized form, reconstitute with the volume of sterile deionized water or buffer specified on the datasheet. Allow the vial to equilibrate to room temperature before reconstitution and mix gently; do not vortex.
• Carrier protein: Addition of a carrier protein (e.g., 0.1% BSA) to the working solution may help stabilize dilute preparations.

Always consult the product-specific datasheet for exact reconstitution volumes, buffer composition, and recommended working concentrations.

Research Applications of Recombinant Human GBX1 Protein

Recombinant Human GBX1 Protein is a versatile reagent applicable across multiple experimental workflows in molecular and cell biology:

• In vitro DNA-binding assays (EMSA): Characterize the sequence-specific DNA-binding activity of the GBX1 homeodomain.
• Protein–protein interaction studies: Use as bait or prey in pull-down assays or surface plasmon resonance to identify GBX1 interacting partners.
• Antibody validation: Serve as a positive control antigen to verify anti-GBX1 antibody specificity in western blot, ELISA, or immunoprecipitation experiments.
• Structural studies: Provide purified protein for crystallography or cryo-EM studies of the homeodomain or full-length protein.
• Functional rescue experiments: Supplement cell-free transcription systems or permeabilized cell models to study GBX1-dependent transcriptional outputs.

Ordering, Shipping, and Returns

Recombinant Human GBX1 Protein (SKU: rp376869) is available for order through Aladdin Scientific's website or by contacting our customer service team. Key ordering and logistics information:

• Ordering: Add SKU rp376869 to your cart or request a formal quotation for institutional procurement. Bulk or custom quantities may be available upon request.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Confirm shipping conditions with Aladdin Scientific at the time of order, particularly for international destinations.
• Returns and refunds: Due to the nature of biological reagents, returns are subject to Aladdin Scientific's standard return and refund policy. Please contact customer service within the applicable window if you have quality concerns upon receipt.
• Payment and pricing: Institutional purchase orders, credit card, and other payment options are accepted. Contact sales for volume pricing or promotional offers.