What Is GBX2 and Why Is It Important?

GBX2 (Gastrulation Brain Homeobox 2) is a member of the homeobox superfamily of transcription factors, characterized by a conserved 60-amino-acid DNA-binding homeodomain. In vertebrates, GBX2 plays a critical role in patterning the developing hindbrain, including the specification of rhombomere identity and the positioning of the midbrain–hindbrain boundary (MHB). It acts in opposition to OTX2 to establish and maintain this boundary, which is essential for the proper development of the cerebellum and brainstem.

Beyond neural development, GBX2 has been implicated in hematopoiesis and has been reported to influence stem cell maintenance. Its dysregulation has been noted in certain cancer contexts, making it a topic of interest for both developmental biology and oncology research.

Product Specifications and Quality

Recombinant Human GBX2 Protein (rp366499) is offered by Aladdin Scientific as a high-quality research reagent. The product is manufactured under controlled conditions to ensure consistency and reproducibility across experiments. Key specification details—such as exact molecular weight, purity percentage, formulation buffer, endotoxin level, and expression host—should be confirmed on the official product datasheet or by contacting Aladdin Scientific technical support, as these parameters are subject to lot-specific variation.

Researchers should request a Certificate of Analysis (CoA) for each lot to verify purity by SDS-PAGE and activity by functional assay prior to use in critical experiments.

Applications of Recombinant Human GBX2 Protein

Recombinant Human GBX2 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–DNA interaction studies: Electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) validation using recombinant protein as a positive control or binding partner.
• Protein–protein interaction assays: Pull-down assays and co-immunoprecipitation experiments to map GBX2 interactome partners.
• Antibody validation: Use as a standard or positive control antigen to validate anti-GBX2 antibodies in Western blot or ELISA.
• Structural and biophysical studies: X-ray crystallography, NMR, or SPR-based binding kinetics with target DNA or partner proteins.
• Cell-free transcription assays: Functional studies in in vitro transcription systems.

Researchers should optimize conditions such as protein concentration, buffer composition, and incubation time for each specific application.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GBX2 Protein are essential to maintain activity and prevent degradation. General best-practice guidelines for recombinant transcription factor proteins include:

• Storage temperature: Store at –80 °C for long-term stability. Avoid repeated freeze–thaw cycles, which can denature the protein and reduce biological activity.
• Aliquoting: Upon receipt, divide the protein into single-use aliquots before freezing to minimize freeze–thaw cycles.
• Working conditions: Keep on ice during bench work. Use within the timeframe recommended on the product datasheet once thawed.
• Carrier protein: If the formulation does not include a stabilizer such as BSA or glycerol, consider adding one at the supplier-recommended concentration to improve stability during freeze–thaw.

Always refer to the specific lot CoA and product datasheet for confirmed storage conditions, as formulations may vary by lot.

Ordering and Support

Recombinant Human GBX2 Protein is available exclusively through Aladdin Scientific under SKU rp366499. To place an order or request a quotation, please visit the Aladdin Scientific website or contact your regional sales representative. For bulk quantities, custom formulations, or project-specific requirements, reach out to the Aladdin Scientific technical sales team.

Technical support, including datasheet requests, Certificate of Analysis (CoA) retrieval, and application guidance, is available through the Aladdin Scientific customer service portal or by emailing the technical support team directly. Researchers requiring safety data should request the Safety Data Sheet (SDS) at the time of inquiry.