What Is GET4 and Why Is It Important?

GET4 (Guided Entry of Tail-anchored proteins 4), also referred to as TRC35 or CGI-116, is a conserved eukaryotic protein that functions as an essential component of the GET (Guided Entry of Tail-anchored proteins) pathway. This pathway is responsible for the post-translational targeting of tail-anchored (TA) membrane proteins to the endoplasmic reticulum (ER). TA proteins are defined by a single transmembrane domain located at or near their C-terminus and include functionally important proteins such as SNAREs, anti-apoptotic Bcl-2 family members, and components of the protein translocation machinery.

GET4 forms a stable heterodimeric complex with GET5 (also known as UBL4A), and this GET4/GET5 complex acts as a co-chaperone that captures newly synthesized TA proteins from the ribosome-associated HSP70 chaperone (SGTA in mammals) and facilitates their transfer to the cytosolic ATPase GET3 (TRC40 in mammals). GET3 then escorts the TA protein to the ER membrane for insertion. Disruption of this pathway leads to mislocalization of TA proteins, proteotoxic stress, and cellular dysfunction.

Product Specifications and Quality

Recombinant Human GET4 Protein (SKU: rp374044) is produced using recombinant expression technology. Researchers should consult the official product datasheet available from Aladdin Scientific for confirmed details on the following parameters, as these may vary by lot:

• Host Expression System: To be confirmed on datasheet (commonly E. coli, baculovirus/insect cell, or mammalian expression systems are used for recombinant proteins of this class).
• Purity: Typically ≥85–95% as assessed by SDS-PAGE; exact purity specification to be confirmed on datasheet.
• Formulation: Supplied in a stabilizing buffer; refer to the datasheet for exact buffer composition, protein concentration, and whether the product is supplied in liquid or lyophilized form.
• Endotoxin Level: Endotoxin content specification to be confirmed on datasheet.

All Aladdin Scientific recombinant proteins are intended for research use only and are quality-controlled prior to release.

Research Applications of Recombinant Human GET4 Protein

Recombinant Human GET4 Protein is a valuable tool for researchers studying TA protein biogenesis, membrane protein targeting, and protein quality control. Key research applications include:

• Biochemical Interaction Studies: Investigating GET4 binding partners, including GET5, GET3, and SGTA, using co-immunoprecipitation, pull-down assays, or surface plasmon resonance.
• Structural Biology: As a component for crystallography or cryo-EM studies of the GET4/GET5 complex or larger GET pathway assemblies.
• In Vitro Reconstitution: Reconstituting the GET pathway in vitro to study the mechanism of TA protein capture and handoff.
• Antibody Validation: Serving as a positive control antigen for validating anti-GET4 antibodies by Western blot, ELISA, or dot blot.
• Enzyme/Functional Assays: Investigating the molecular chaperone activity and ATPase-stimulating functions associated with GET4-containing complexes.

Researchers are encouraged to optimize assay conditions for their specific experimental systems.

Storage, Handling, and Stability

Proper storage and handling are critical to maintaining the activity and integrity of Recombinant Human GET4 Protein. General best-practice recommendations for recombinant proteins apply:

• Short-term storage: Store at 4°C if the protein will be used within 1–2 weeks of receipt.
• Long-term storage: Aliquot and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Freeze-thaw cycles: Minimize to no more than 2–3 cycles. Consider adding a carrier protein such as 0.1% BSA if working at very low concentrations.
• Handling: Keep on ice during use. Avoid prolonged exposure to room temperature.

Note: Always refer to the lot-specific Certificate of Analysis (CoA) and product datasheet for storage conditions confirmed by Aladdin Scientific, as formulation details may affect optimal storage temperature.

Ordering and Technical Support

Recombinant Human GET4 Protein is available from Aladdin Scientific under SKU rp374044. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative. The following resources are available to support your purchase and experimental work:

• Product Datasheet: Available for download on the product page; contains confirmed expression system, purity, formulation, and quality control data.
• Certificate of Analysis (CoA): Lot-specific CoA available upon request or via the Aladdin Scientific portal.
• Technical Support: Aladdin Scientific technical specialists can assist with application recommendations, protocol optimization, and troubleshooting.
• Returns and Refunds: Please refer to Aladdin Scientific's returns and refunds policy for eligibility criteria regarding recombinant proteins.

For bulk quantities or custom formulations, please contact Aladdin Scientific directly to discuss your requirements.