Protein Background and Biology

GGT2P (Gamma-Glutamyltransferase 2 Pseudogene) is a member of the gamma-glutamyltransferase gene family in humans. GGT enzymes canonically catalyze the transfer of the gamma-glutamyl moiety from glutathione and related peptides to amino acid acceptors, playing central roles in glutathione homeostasis, amino acid transport, and cellular antioxidant defense. The GGT gene family in humans includes multiple functional members (e.g., GGT1, GGT5, GGT6, GGT7) as well as pseudogene-related loci such as GGT2P.

Although GGT2P has been classified as a pseudogene locus, recombinant protein products derived from or related to this locus are valuable research tools for studying GGT family structure-function relationships, cross-reactivity of antibodies across GGT family members, and evolutionary divergence within the gene family. Researchers should consult current genomic and proteomic databases to confirm the precise sequence and functional status of this locus in their experimental context.

Reconstitution and Storage Protocol

Proper reconstitution and storage of Recombinant Human GGT2P protein are essential to maintain protein integrity and biological activity. Follow these general guidelines, and always refer to the specific Certificate of Analysis (CoA) supplied with your lot for lot-specific instructions:

• Reconstitution: Briefly centrifuge the vial before opening to collect lyophilized material at the bottom. Reconstitute in sterile, ultrapure water or an appropriate buffer (e.g., PBS, pH 7.4) as specified in the CoA. Gently pipette to mix; avoid vortexing to prevent protein denaturation.
• Working concentration: Prepare aliquots at your desired working concentration immediately after reconstitution to minimize freeze-thaw cycles.
• Short-term storage: Store reconstituted protein at 4 °C for up to 1 week when in frequent use.
• Long-term storage: For extended storage, add a carrier protein (e.g., 0.1% BSA) if recommended by the CoA, aliquot, and store at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles, as these reduce protein activity.
• Lyophilized form: Store lyophilized protein at -20 °C, desiccated. Lyophilized material is stable for the period indicated on the product label when stored correctly.

Note: Specific buffer compositions, reconstitution volumes, and stability data are provided on the product-specific CoA. Always consult the CoA prior to use.

Experimental Applications

Recombinant Human GGT2P protein (rp372923) is intended for research use in a variety of biochemical and cell biology applications. Typical applications for recombinant proteins of this class include:

• SDS-PAGE and Western Blotting: Use as a positive control or molecular weight marker for GGT family proteins. Confirm band migration relative to expected molecular weight as indicated on the CoA.
• Antibody Validation: Serve as a defined antigen to test specificity and cross-reactivity of anti-GGT antibodies, particularly for distinguishing between GGT family members.
• ELISA Development: Use as a coating antigen or standard in sandwich or competitive ELISA formats for GGT2P detection or quantification assays.
• Protein Interaction Studies: Employ in pull-down assays, co-immunoprecipitation, or surface plasmon resonance (SPR) experiments to identify binding partners of GGT family members.
• Biochemical Assays: Investigate substrate specificity, inhibitor screening, or enzyme kinetics if enzymatic activity is confirmed for this protein (see CoA for activity data).

Optimal working concentrations should be determined empirically for each application. Refer to the product CoA for confirmed activities and quality specifications.

Quality, Safety, and Regulatory Notes

Quality Control: Each lot of Recombinant Human GGT2P protein undergoes quality control testing prior to release. Typical QC tests for recombinant proteins include SDS-PAGE purity assessment and, where applicable, activity assays. Specific QC data are documented on the lot-specific Certificate of Analysis (CoA), available upon request from Aladdin Scientific.

Purity: The expected purity grade for this recombinant protein is indicated on the CoA. Researchers should confirm purity specifications match their experimental requirements prior to use.

Safety: Handle all recombinant proteins in accordance with institutional biosafety guidelines. Wear appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection. Consult the Safety Data Sheet (SDS) for hazard information before use.

Regulatory: This product is for Research Use Only (RUO). It is not approved for use in diagnostic procedures, clinical applications, food, drug, or cosmetic manufacturing. Users are responsible for ensuring compliance with all applicable local and national regulations governing the use of recombinant proteins in their jurisdiction.