Biological Background of GLDN (Gliomedin)

GLDN, commonly known as Gliomedin, is an extracellular matrix protein predominantly expressed by myelinating Schwann cells in the peripheral nervous system (PNS). It belongs to the olfactomedin domain-containing protein family and plays an essential role in the formation and maintenance of nodes of Ranvier — specialized axonal segments critical for saltatory conduction of nerve impulses.

Gliomedin interacts with neuronal cell-adhesion molecules NrCAM and neurofascin-186 (NF186) clustered at the nodal axolemma, thereby anchoring and stabilizing nodal components during myelination. Studies have demonstrated that disruption of GLDN function impairs proper node formation and axonal excitability. Research interest in GLDN also extends to autoimmune peripheral neuropathies, where anti-GLDN antibodies have been detected in some patients with Guillain–Barré syndrome.

Reconstitution and Handling Guidelines

Proper reconstitution and handling of Recombinant Human GLDN Protein (rp364946) are essential to preserve protein activity and ensure reproducible experimental results. Follow the guidelines below:

• Reconstitution: Centrifuge the vial briefly before opening to consolidate lyophilized material. Reconstitute in sterile, ultrapure water or an appropriate buffer (such as PBS, pH 7.4) to the desired concentration. Allow the protein to dissolve gently; avoid vigorous vortexing.
• Working concentration: Determine empirically for each assay. Carrier protein (e.g., 0.1% BSA) may be added to improve stability in dilute solutions.
• Avoid contamination: Use sterile technique and low-protein-binding tubes throughout.
• Handling temperature: Keep the reconstituted protein on ice during use to minimize degradation.

Always consult the product datasheet (Absea #PP-25358) for lot-specific concentration, purity, and formulation details.

Storage, Stability, and Quality Control

Storage conditions: Store lyophilized Recombinant Human GLDN Protein at -20°C for routine use or at -80°C for long-term storage. Once reconstituted, aliquot into single-use volumes to prevent repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.

Stability: Lyophilized protein is generally stable for up to 12 months from the date of receipt when stored under recommended conditions. Reconstituted protein should ideally be used within 48–72 hours when kept at 4°C, or stored frozen in aliquots for extended periods.

Quality control: Aladdin Scientific provides lot-specific certificates of analysis (CoA) confirming purity (typically assessed by SDS-PAGE) and biological activity where applicable. Consult the CoA supplied with each lot and the referenced Absea datasheet (#PP-25358) for precise specifications.

Recommended Research Applications

Recombinant Human GLDN Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: Investigating GLDN binding to NrCAM, neurofascin, and heparan sulfate proteoglycans.
• Cell-adhesion assays: Evaluating the adhesive properties of Gliomedin with Schwann cells or neuronal cell lines.
• ELISA and immunoassay development: As a standard or coating antigen for detection of anti-GLDN antibodies in serum samples relevant to peripheral neuropathy research.
• Structural and biochemical studies: Characterizing the olfactomedin domain and coiled-coil regions of GLDN.
• Functional rescue experiments: Supplementing GLDN-deficient in vitro systems to study nodal organization.

This product is for research use only and is not intended for clinical, diagnostic, or therapeutic applications.