What Is GLRA2 and Why Is It Important in Research?

GLRA2 (Glycine Receptor Alpha-2 Subunit) is a ligand-gated ion channel subunit that forms part of the inhibitory glycine receptor complex, a critical mediator of inhibitory neurotransmission in the central nervous system (CNS). Glycine receptors are pentameric chloride channels activated by glycine, and the alpha-2 subunit is particularly prominent during early neuronal development, where it plays roles in synaptogenesis and network formation.

Research into GLRA2 is relevant to understanding conditions such as hyperekplexia (startle disease), autism spectrum disorders, and other neurological conditions associated with dysregulation of inhibitory signaling. Recombinant Human GLRA2 Protein enables researchers to study receptor structure, ligand-binding properties, and protein–protein interactions in a controlled in vitro setting.

Product Specifications and Quality Information

Recombinant Human GLRA2 Protein (SKU: rp370069) is produced recombinantly to deliver consistent, research-grade quality. Key specification details users should confirm with Aladdin Scientific at the time of ordering include:

• Expression system: Please consult the product datasheet for the confirmed host expression system (e.g., E. coli, HEK293, baculovirus/insect cell, or wheat germ). The choice of expression system affects post-translational modifications and protein folding.
• Purity: Recombinant proteins of this class are typically supplied at >85–95% purity as assessed by SDS-PAGE; confirm exact purity on the current product datasheet.
• Formulation and buffer: The protein is typically supplied lyophilized or in aqueous buffer; refer to the current datasheet for exact formulation details.
• Endotoxin levels: For functional cell-based assays, endotoxin content is a critical quality parameter; consult the Certificate of Analysis (CoA) for lot-specific values.

All specific numerical values (purity %, concentration, endotoxin limits) should be verified against the current product datasheet and CoA available from Aladdin Scientific.

Research Applications of Recombinant Human GLRA2 Protein

Recombinant Human GLRA2 Protein is suited for a range of in vitro research applications where investigators require a defined, purified source of the GLRA2 antigen or active protein. Common research uses include:

• Antibody development and validation: Used as an antigen for generating or validating antibodies targeting the GLRA2 subunit.
• Binding and interaction studies: Supports biochemical assays such as ELISA, surface plasmon resonance (SPR), and pull-down experiments to characterize ligand or protein interactions.
• Structural biology: May serve as a component in X-ray crystallography or cryo-EM studies aimed at resolving glycine receptor subunit structure.
• Drug discovery screening: Can be employed in assay development for identifying small molecules that modulate glycine receptor activity.
• Western blot positive control: Provides a well-defined positive control band for immunodetection experiments.

Researchers should consult the product datasheet to confirm which specific applications have been validated by the manufacturer.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling are essential to maintaining the activity and integrity of Recombinant Human GLRA2 Protein. General best-practice guidelines for recombinant proteins of this type include:

• Long-term storage: Lyophilized proteins are typically stable at –20 °C or –80 °C; liquid formulations are generally stored at –80 °C for long-term use. Always refer to the specific product datasheet for confirmed storage temperatures.
• Avoid repeated freeze-thaw cycles: Aliquot the protein upon first thaw to minimize activity loss due to repeated freeze-thaw cycles.
• Reconstitution: If supplied in lyophilized form, reconstitute with the buffer specified in the datasheet (typically sterile water or PBS). Allow the lyophilized cake to equilibrate to room temperature before opening to prevent moisture condensation.
• Working solutions: Prepare working dilutions fresh on the day of use in a compatible assay buffer. Carrier proteins such as BSA (0.1%) may help stabilize dilute protein solutions.

Specific storage conditions, shelf life, and reconstitution volumes must be confirmed from the current Aladdin Scientific product datasheet and CoA for lot rp370069.

Ordering and Technical Support

Recombinant Human GLRA2 Protein is available from Aladdin Scientific under SKU rp370069. When placing an order or requesting further information, customers are encouraged to:

• Request the current product datasheet and Certificate of Analysis (CoA) to verify lot-specific quality data before use.
• Contact Aladdin Scientific technical support for application-specific recommendations, custom formulation inquiries, or bulk pricing.
• Confirm current stock availability and lead times through the Aladdin Scientific website or sales team, as availability may vary.
• Review the returns, refunds, and shipping policies on the Aladdin Scientific website to understand handling requirements for temperature-sensitive biological reagents.

For the most up-to-date pricing, availability, and datasheet information, visit the Aladdin Scientific product page for rp370069 or contact your regional sales representative.