What Is GLRX3 and Why Is It Important in Research?

GLRX3, also known as glutaredoxin-3 or PICOT (PKC-interacting cousin of thioredoxin), is a multidomain glutaredoxin belonging to the thioredoxin superfamily. It contains one thioredoxin-like domain and two glutaredoxin domains, making it structurally distinct from classical monodomain glutaredoxins.

GLRX3 plays a pivotal role in maintaining cellular redox homeostasis. It participates in iron-sulfur (Fe-S) cluster assembly and delivery, functioning as a scaffold or transfer protein that helps incorporate Fe-S clusters into target apoproteins. This function is critical for mitochondrial and cytosolic iron metabolism.

Beyond iron metabolism, GLRX3 has been implicated in the regulation of cardiac hypertrophy, cell proliferation, and the cellular response to oxidative stress. Its interaction with protein kinase C (PKC) and other signaling partners positions it as a node in multiple redox-sensitive signaling pathways, making it a compelling target for studies in cardiovascular biology, cancer biology, and metabolic disease research.

How Should Recombinant Human GLRX3 Protein Be Stored and Reconstituted?

Proper storage and reconstitution are essential to maintaining the activity and integrity of Recombinant Human GLRX3 Protein. As general best-practice guidelines for recombinant proteins of this class apply, users should follow the specific conditions stated on the product datasheet supplied with SKU rp377138, as formulation details may vary by lot.

Storage: Recombinant proteins are typically stored at −20°C for short-term use or at −80°C for long-term preservation. Repeated freeze-thaw cycles should be avoided, as they can lead to protein aggregation and loss of activity. Aliquoting the protein upon first thaw is strongly recommended.

Reconstitution: If supplied in lyophilized form, the protein should be reconstituted by adding the recommended volume of sterile deionized water or the buffer specified in the datasheet. Allow the solution to sit for several minutes at room temperature and gently mix without vortexing to avoid denaturation. Centrifuge briefly before use to collect any residual material.

Working Conditions: Once reconstituted, keep the protein on ice during experiments. If not used within a few hours, re-aliquot and store at −80°C. Refer to the product-specific datasheet for exact buffer composition, recommended concentrations, and stability data.

What Applications Is Recombinant Human GLRX3 Protein Suitable For?

Recombinant Human GLRX3 Protein (rp377138) is intended for in vitro research applications. Based on the known biology of GLRX3 and the general utility of recombinant glutaredoxin proteins, the following applications are commonly supported:

• Protein–Protein Interaction Studies: GLRX3 interacts with multiple partners including BOLA proteins and PKC; recombinant GLRX3 can be used in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance experiments.
• Fe-S Cluster Assembly Assays: GLRX3 serves as an Fe-S cluster scaffold; the recombinant protein can be employed in reconstitution assays to study cluster transfer to target proteins.
• Redox Activity Assays: Glutaredoxin activity can be assessed using standard spectrophotometric assays measuring GSH-dependent reduction of substrates such as hydroxyethyl disulfide (HED).
• Antibody Validation: The purified recombinant protein serves as a positive control or antigen standard for western blotting, ELISA development, and antibody specificity confirmation.
• Structural Studies: High-purity recombinant GLRX3 may be suitable as a reference standard in biophysical characterization methods such as circular dichroism or analytical size-exclusion chromatography.

Users should validate the protein for their specific assay conditions. Not intended for human therapeutic or diagnostic use.

Quality, Purity, and Expression System Considerations

When selecting a recombinant protein for critical assays, understanding the expression system, purity, and quality-control (QC) testing performed is essential. For Recombinant Human GLRX3 Protein (rp377138), users should consult the product-specific Certificate of Analysis (CoA) and datasheet available from Aladdin Scientific for lot-specific data.

Expression Systems: Recombinant proteins may be expressed in bacterial (E. coli), insect (baculovirus/Sf9), mammalian (HEK293 or CHO), or yeast systems. Each system has implications for post-translational modifications, folding, and activity. The specific expression host for rp377138 should be confirmed on the datasheet.

Purity: Research-grade recombinant proteins are generally purified to ≥85–95% purity as assessed by SDS-PAGE. The exact purity specification for rp377138 should be verified in the CoA.

Endotoxin: For cell-based assays or sensitive functional experiments, endotoxin levels are critical. Users should check whether endotoxin testing (LAL assay) has been performed and confirm the specification meets their assay requirements.

Biological Activity: Where applicable, functional activity data (e.g., EC50 values from activity assays) may be provided on the datasheet. Contact Aladdin Scientific technical support for specific QC documentation.

How to Order and What to Expect from Aladdin Scientific

Recombinant Human GLRX3 Protein is available from Aladdin Scientific under SKU rp377138. Ordering is straightforward through the Aladdin Scientific website or by contacting our sales team directly.

Ordering Process: Search for SKU rp377138 on the Aladdin Scientific product portal. Select the required quantity, add to cart, and proceed through the standard checkout. Purchase orders from institutions are accepted; contact the sales team for invoicing arrangements.

Shipping: Recombinant proteins are temperature-sensitive and are shipped on dry ice or with appropriate cold-chain packaging to maintain integrity during transit. Delivery timelines vary by region; consult the shipping and delivery policy on the Aladdin Scientific website for specifics.

Returns and Technical Support: If the product does not meet quality specifications upon receipt, contact Aladdin Scientific customer service with the lot number and CoA for resolution. Technical support is available to assist with reconstitution, storage, and application-related questions.

Bulk and Custom Requests: For large-scale or custom formulation requirements, contact our sales or technical team to discuss options including bulk pricing, endotoxin-removal services, or custom buffer formulations.