What Is GMNC and Why Is It Important?

GMNC, also known as Geminin Coiled-Coil Domain Containing protein (also referred to as GemC1 in the literature), is a nuclear protein that plays a central role in the initiation of multiciliogenesis — the developmental process by which epithelial progenitor cells differentiate into multiciliated cells bearing hundreds of motile cilia on their surface.

GMNC functions as a transcriptional activator by interacting with E2F4/E2F5 transcription factors and their dimerization partners (DP proteins) to drive the transcriptional program required for multiciliated cell fate specification. Multiciliated cells are essential for mucociliary clearance in the airway, cerebrospinal fluid flow in the brain, and egg transport in the reproductive tract. Loss of GMNC function has been associated with defects in multiciliated cell differentiation, making it a relevant target in studies of ciliopathies, airway biology, and developmental biology.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human GMNC Protein (SKU: rp366233, reference absea#PP-1850) is produced using recombinant expression systems to deliver a high-quality protein reagent suitable for in vitro research applications. Key specification details should be confirmed on the product datasheet, as parameters such as molecular weight, tag configuration, expression host, purity grade, and endotoxin level may vary by lot.

Researchers should consult the Certificate of Analysis (CoA) supplied with each lot for confirmed purity (typically assessed by SDS-PAGE), biological activity data where available, and endotoxin testing results. For precise molecular weight, isoelectric point, amino acid sequence coverage, and formulation details, please refer to the official product page or contact Aladdin Scientific technical support.

Storage, Handling, and Reconstitution

Proper storage and handling of recombinant proteins is critical to maintaining activity and integrity. As a general best practice for recombinant proteins of this class:

• Short-term storage: Store at 4 °C if the protein will be used within a few weeks of receipt.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can degrade protein activity and increase aggregation.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended on the datasheet (typically sterile PBS or similar). Allow the vial to equilibrate to room temperature before opening.
• Carrier protein: Addition of a carrier protein such as 0.1% BSA may help stabilize dilute protein solutions.

Always refer to the specific product datasheet and CoA for lot-specific storage and reconstitution instructions, as conditions may differ from general guidelines.

Research Applications

Recombinant Human GMNC Protein is intended for research use only and may be applicable in a range of in vitro studies, including but not limited to:

• Protein–protein interaction studies: Investigating GMNC interactions with E2F transcription factors, DP proteins, or other components of the multiciliogenesis transcriptional network.
• Biochemical assays: Use as a standard or positive control in Western blotting or ELISA-based detection assays.
• Structural studies: Supporting structural biology investigations of the GMNC coiled-coil domain.
• Antibody validation: Serving as a positive control antigen for validating anti-GMNC antibodies.
• Functional assays: Investigating the transcriptional activation properties of GMNC in cell-free or reconstituted systems.

Researchers studying ciliopathies, airway epithelial biology, neurodevelopment (ependymal cilia), or reproductive biology may find this protein particularly relevant.

Ordering, Support, and Regulatory Information

How to order: Recombinant Human GMNC Protein is available from Aladdin Scientific under SKU rp366233. Place orders through the Aladdin Scientific website, by contacting your regional sales representative, or via your institution's approved procurement channel.

Technical support: For application-specific guidance, datasheet requests, bulk pricing, or custom formulation inquiries, please contact Aladdin Scientific's technical support team directly.

Regulatory status: This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical use in humans or animals.

Returns and quality concerns: If you receive a product that does not meet specifications, please contact Aladdin Scientific customer service within the timeframe specified in the return policy, referencing your order number and lot number.