What Is GOLGA6B and Why Is It Studied?

GOLGA6B (Golgi Autoantigen, Golgin Subfamily A Member 6B) is a member of the golgin protein family, a group of coiled-coil proteins that localize to the Golgi apparatus and play critical roles in maintaining Golgi structure, membrane tethering, and vesicular trafficking. Golgins act as long, flexible tethers that help capture transport vesicles and facilitate their fusion with target membranes within the secretory pathway.

GOLGA6B belongs to the GOLGA6 subfamily, which has expanded through segmental duplication in the human genome, making it of particular interest in studies of genome evolution, copy-number variation, and Golgi biogenesis. Research into GOLGA6B and related family members may provide insights into disorders associated with Golgi dysfunction and intracellular trafficking defects.

Recombinant Human GOLGA6B Protein (rp377472) enables researchers to conduct biochemical characterization, protein–protein interaction studies, and antibody validation in a controlled, reproducible manner.

Storage, Handling, and Reconstitution

Storage: Recombinant proteins are generally sensitive to repeated freeze–thaw cycles. Unless otherwise stated in the product datasheet, the lyophilized form should be stored at –20 °C or –80 °C, while liquid formulations are typically stored at –80 °C. Always refer to the specific lot certificate of analysis (CoA) supplied with the product for confirmed storage conditions.

Reconstitution: For lyophilized preparations, allow the vial to equilibrate to room temperature before opening. Reconstitute in sterile deionized water or an appropriate buffer (e.g., PBS containing a carrier protein such as 0.1% BSA) to the recommended concentration indicated on the datasheet. Gently mix by pipetting or inverting; do not vortex vigorously, as this may denature the protein.

Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze–thaw degradation. Working aliquots may be stored at 4 °C for short-term use (typically up to one week), while long-term stock aliquots should be stored at –80 °C.

Note: Specific buffer composition, concentration, and excipients for rp377472 should be confirmed from the product datasheet or by contacting Aladdin Scientific technical support.

Recommended Applications

Recombinant Human GOLGA6B Protein is intended for research use and may be suitable for the following applications, subject to validation by the end user:

• In vitro biochemical assays: Including binding assays, ELISA-based interaction studies, and enzyme-linked functional assays where GOLGA6B serves as a capture or detection antigen.
• Antibody validation and immunoassay development: As a positive control antigen for Western blot, dot blot, or ELISA to confirm antibody specificity against GOLGA6B.
• Protein–protein interaction studies: Pull-down assays or co-immunoprecipitation studies to identify binding partners of GOLGA6B within vesicular trafficking complexes.
• Structural and biophysical studies: Circular dichroism, size-exclusion chromatography, or thermal stability assays to characterize the folding and stability of the recombinant protein.

Note: End users are responsible for validating the suitability of this product for their specific experimental systems. Application performance may vary depending on experimental conditions.

Quality, Purity, and Regulatory Status

Purity: Recombinant proteins from Aladdin Scientific are manufactured and quality-tested to research-grade standards. Purity is typically assessed by SDS-PAGE and/or HPLC. Specific purity data for rp377472 should be confirmed from the product CoA.

Endotoxin: Endotoxin levels are assessed by LAL (Limulus Amebocyte Lysate) assay for applicable products. Refer to the product datasheet for the endotoxin specification for rp377472.

Regulatory Status: This product is supplied for research use only (RUO) and is not intended for use in diagnostic procedures, clinical trials, or therapeutic applications in humans or animals. It does not carry CE, FDA 510(k), or IVD marking.

Lot-to-lot consistency: Each production lot is tested against defined release specifications. Researchers requiring lot reservation or specific lot documentation should contact Aladdin Scientific customer service.

Ordering and Customer Support

How to Order: Recombinant Human GOLGA6B Protein (rp377472) can be ordered directly through the Aladdin Scientific website or by contacting your regional sales representative. Provide the SKU rp377472 when placing your order to ensure accurate fulfillment.

Lead Time: Lead times for recombinant proteins may vary. Please confirm current stock availability and estimated delivery at the time of ordering. Aladdin Scientific offers standard and expedited shipping options where available.

Technical Support: For technical questions regarding reconstitution protocols, application suitability, or experimental troubleshooting, contact Aladdin Scientific's technical support team with your order number and lot number for the most accurate assistance.

Returns and Refunds: Returns are accepted in accordance with Aladdin Scientific's standard return policy. Products must be returned within the specified return window and in their original condition. Contact customer service to initiate a return authorization.