Background: The GOLGA6C Gene and Protein

GOLGA6C (Golgin A6 Family Member C) is a member of the Golgin subfamily of coiled-coil proteins that are predominantly localized to the Golgi apparatus. Golgins are a structurally diverse group of peripheral membrane proteins that play critical roles in maintaining Golgi morphology, mediating vesicular tethering, and regulating intracellular membrane trafficking.

The GOLGA6 gene family in humans includes several paralogs arising through gene duplication events, and GOLGA6C represents one such paralog. Like other Golgins, GOLGA6C is predicted to form extended coiled-coil domains that facilitate protein-protein interactions at the Golgi membrane surface. Understanding the precise molecular functions of GOLGA6C and its paralogs remains an active area of investigation in cell biology.

Biochemical Properties and Specifications

Recombinant Human GOLGA6C Protein is produced using recombinant expression systems to yield a product suitable for in vitro research applications. As a member of the coiled-coil Golgin family, GOLGA6C contains characteristic heptad repeat sequences that drive alpha-helical coiled-coil formation, a structural motif essential for its biological interactions.

Researchers should consult the specific product datasheet for Aladdin Scientific SKU rp377471 for confirmed details on expression host, molecular weight, purity grade, formulation buffer, and storage conditions, as these parameters are critical for experimental reproducibility. All lot-specific quality control data are available upon request.

Research Applications

Recombinant Human GOLGA6C Protein is suited for a range of in vitro research applications in cell biology and biochemistry, including but not limited to:

• Protein interaction studies: Identifying binding partners of GOLGA6C within the Golgi tethering machinery using pull-down or co-immunoprecipitation assays.
• Antibody development and validation: Serving as an antigen for generating or validating antibodies specific to GOLGA6C.
• Structural and biophysical analyses: Supporting studies of coiled-coil domain architecture by circular dichroism or analytical ultracentrifugation.
• Functional assays: Investigating the role of GOLGA6C in membrane trafficking reconstitution experiments.
• Proteomics research: Use as a reference standard or spike-in control for mass spectrometry-based proteomics workflows.

The suitability of this protein for specific applications such as ELISA, Western blot, or functional bioassays should be confirmed by consulting the product datasheet.

Storage, Handling, and Quality Assurance

Proper handling of recombinant proteins is essential to maintain activity and ensure data reproducibility. General best practices for recombinant proteins of this class include:

• Store lyophilized or liquid protein preparations according to the conditions specified on the product datasheet (typically -20°C or -80°C for long-term storage).
• Avoid repeated freeze-thaw cycles; prepare single-use aliquots upon first reconstitution.
• Reconstitute lyophilized protein in the recommended buffer and allow complete dissolution before use.
• Centrifuge briefly before opening to ensure material is collected at the bottom of the vial.

Aladdin Scientific performs quality control testing on each production lot. Researchers requiring a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) should contact Aladdin Scientific customer support or download documentation directly from the product page for SKU rp377471.

Ordering Information

Recombinant Human GOLGA6C Protein is available from Aladdin Scientific under the following catalog information:

For pricing, available pack sizes, and current stock status, please visit the Aladdin Scientific website or contact your regional sales representative. Volume discounts may be available for larger orders.