Background: The GOLGA6L19 Gene and Golgin Protein Family

GOLGA6L19 (Golgin A6 Family-Like 19) is a member of the extended GOLGA6 gene family, which belongs to the broader superfamily of Golgins — a class of predominantly coiled-coil proteins that associate with the cytoplasmic face of the Golgi apparatus. Golgins are well established as structural scaffolds and tethering factors that maintain Golgi morphology and facilitate vesicle capture during intracellular membrane trafficking.

The GOLGA6 subfamily is encoded by a group of paralogous genes that arose through segmental duplication events on human chromosome 15, resulting in numerous family members and pseudogenes. GOLGA6L19 is one such member; however, detailed functional characterization of this specific paralog remains limited in the published literature, making recombinant protein tools particularly valuable for investigative research.

As a Golgi-associated protein, GOLGA6L19 may participate in the regulation of secretory pathway dynamics, Golgi ribbon formation, or other membrane-tethering events, consistent with canonical Golgin biology. Researchers studying Golgi biogenesis, organelle stress responses, or vesicle trafficking may find this protein relevant to their experimental models.

Product Specifications and Biochemical Properties

Aladdin Scientific's Recombinant Human GOLGA6L19 Protein (SKU: rp376271) is produced as a recombinant protein for research applications. As with all recombinant proteins in the Aladdin portfolio, this product is manufactured under controlled conditions designed to deliver consistent lot-to-lot quality suitable for experimental reproducibility.

Key specification parameters — including the expression host system (e.g., E. coli, baculovirus/insect cell, mammalian, or wheat germ cell-free), affinity tag identity and position, apparent molecular weight, purity grade (typically assessed by SDS-PAGE and/or HPLC), endotoxin level, and formulation buffer — are detailed in the official product datasheet for rp376271. Researchers are strongly advised to consult the current datasheet prior to experimental use to ensure compatibility with downstream assay requirements.

The protein is supplied in research-use-only (RUO) grade and is not intended for clinical, diagnostic, or therapeutic applications. Lot-specific certificates of analysis (CoA) are available upon request and provide quantitative data on purity, concentration, and activity where applicable.

Research Applications

Recombinant Human GOLGA6L19 Protein is intended to support a range of in vitro and cell-free research applications consistent with the study of Golgi-associated coiled-coil proteins. Typical applications for recombinant Golgin-family proteins include:

• Protein–protein interaction studies: Pull-down assays, co-immunoprecipitation controls, and yeast two-hybrid validation using purified recombinant protein as bait or prey to identify binding partners involved in vesicle tethering or Golgi organization.
• Antibody validation and immunoassay development: Use as a positive control antigen to validate anti-GOLGA6L19 antibodies in Western blot, ELISA, or immunofluorescence experiments.
• Structural and biophysical characterization: Circular dichroism (CD), dynamic light scattering (DLS), or limited proteolysis to characterize the coiled-coil domain architecture of the recombinant protein.
• Functional reconstitution experiments: Cell-free reconstitution of Golgi tethering or membrane fusion events to probe the biochemical contribution of GOLGA6L19.
• Biomarker and proteomics research: As a reference standard in mass spectrometry-based proteomics workflows targeting the GOLGA6 protein family.

Specific validated applications for rp376271 are listed in the product datasheet. Researchers should verify suitability for each intended application prior to use.

Handling, Storage, and Experimental Considerations

Proper handling of recombinant proteins is essential to maintain activity and ensure experimental reliability. The following general best-practice guidelines apply to Recombinant Human GOLGA6L19 Protein (rp376271), though researchers must refer to the product-specific datasheet for authoritative storage and reconstitution instructions:

• Storage temperature: Recombinant proteins are typically shipped on dry ice and stored at −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, which can cause aggregation and activity loss. Aliquot upon first thaw.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified in the datasheet (commonly sterile PBS or a carrier protein-containing buffer). Allow the vial to equilibrate to room temperature before opening.
• Working concentration: Optimize working concentrations empirically for each assay format. Pilot dose-response experiments are recommended before committing to large-scale studies.
• Carrier protein: For dilute solutions, addition of a carrier protein (e.g., 0.1% BSA) to the diluent can prevent adsorptive losses to tube surfaces.
• Contamination control: Use aseptic technique throughout; microbial contamination can degrade the protein and confound results.

Contact Aladdin Scientific technical support for application-specific handling advice or if anomalous results are observed.