Background: The GOLGA6L4 Gene and Protein

GOLGA6L4 (Golgi Autoantigen, Golgin Subfamily A Member 6-Like 4) is a member of the Golgin protein superfamily, a group of large coiled-coil proteins predominantly localized to the Golgi apparatus. Golgins are characterized by extensive regions of predicted coiled-coil secondary structure and play essential roles in maintaining Golgi architecture, mediating vesicle tethering, and facilitating intracellular membrane trafficking. The GOLGA6 subfamily, to which GOLGA6L4 belongs, has expanded in the human genome through duplication events and is associated with copy-number variation regions, making members of this subfamily of particular interest in genomic and proteomic research.

The GOLGA6L4 gene is located in a region of the genome prone to segmental duplication, and its expression and functional roles continue to be characterized. Research into GOLGA6L4 and related Golgin subfamily members contributes to understanding Golgi biogenesis, vesicular transport, and the molecular basis of diseases linked to Golgi dysfunction.

Product Overview and Specifications

Aladdin Scientific's Recombinant Human GOLGA6L4 Protein (SKU: rp377473) is produced using recombinant DNA technology to yield a human-sequence protein suitable for research applications. Recombinant production ensures batch-to-batch consistency and eliminates the variability associated with protein purification from native tissue sources. The protein is intended for research use only and is not approved for diagnostic or therapeutic applications.

Researchers should consult the product datasheet for confirmed expression system, host organism, tag information, purity grade, and formulation details. Standard quality control testing for recombinant proteins typically includes SDS-PAGE analysis to confirm molecular weight, and functional or binding assays where applicable. For specific lot-level data including purity percentage and endotoxin levels, refer to the Certificate of Analysis (CoA) available upon request from Aladdin Scientific.

Research Applications

Recombinant Human GOLGA6L4 Protein is suited for a range of in vitro and cell-based research applications, including but not limited to:

• Antibody development and validation: Used as an immunogen or positive control antigen for generating and screening antibodies against GOLGA6L4 epitopes.
• Protein interaction studies: Employed in pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) experiments to identify binding partners of GOLGA6L4.
• Functional assays: Applied in biochemical studies investigating the role of GOLGA6L4 in Golgi architecture and vesicular trafficking pathways.
• Proteomics and structural biology: Useful as a reference standard in mass spectrometry-based proteomics or as a substrate for structural characterization studies.
• ELISA and Western blot standards: Serves as a quantitative standard or positive control in immunoassay development and optimization.

Researchers should validate the protein's suitability for each specific application in their own experimental system, as performance may vary depending on assay conditions.

Handling, Storage, and Stability

Proper handling and storage of recombinant proteins are critical to preserving activity and ensuring reproducible experimental results. The following general guidelines apply to Recombinant Human GOLGA6L4 Protein:

• Upon receipt: Inspect the product immediately. If the protein is supplied lyophilized, briefly centrifuge the vial before opening to collect material at the bottom. If supplied in liquid form, place on ice during use.
• Reconstitution (lyophilized format): Reconstitute in the buffer recommended on the product datasheet. Allow the solution to equilibrate for several minutes and mix gently; avoid vortexing, which can denature the protein.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution or upon first thaw to minimize repeated freeze-thaw cycles, which degrade protein integrity and activity.
• Storage temperature: Store aliquots at -80°C for long-term stability. Short-term storage at -20°C may be acceptable; refer to the product datasheet for confirmed stability data.
• Avoid: Repeated freeze-thaw cycles, prolonged exposure to room temperature, and storage in frost-free freezers that cycle in temperature.

Always consult the product-specific datasheet and CoA for confirmed storage conditions, as formulation details may vary by lot.

Ordering and Technical Support

Recombinant Human GOLGA6L4 Protein is available from Aladdin Scientific under SKU rp377473. To place an order or request a bulk quotation, contact your regional Aladdin Scientific representative or use the online ordering portal. For technical inquiries including application-specific guidance, buffer compatibility, or custom formulation requests, reach out to Aladdin Scientific's technical support team.

When ordering, please specify the required quantity and desired format (e.g., lyophilized or liquid). A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available for each lot upon request. Aladdin Scientific is committed to providing high-quality research reagents supported by rigorous quality control and responsive customer service.