Background: GOLGA8B and the Golgin Protein Family

GOLGA8B (Golgi Autoantigen, Golgin Subfamily A, Member 8B) is a member of the golgin family of coiled-coil proteins that are associated with the Golgi apparatus. Golgins play essential roles in maintaining Golgi structure, mediating vesicle tethering, and regulating intracellular membrane trafficking. GOLGA8B is encoded by the GOLGA8B gene in humans and belongs to a subfamily characterized by extended coiled-coil domains that enable protein–protein interactions at the Golgi membrane.

The Golgi apparatus serves as the central hub for processing, sorting, and dispatching proteins and lipids within eukaryotic cells. Disruption of golgin function has been implicated in various cellular dysfunctions, making recombinant golgin-family proteins valuable tools for investigating membrane trafficking pathways, organelle biogenesis, and related disease mechanisms.

Note: Detailed molecular weight, isoelectric point, and specific expression data for GOLGA8B should be confirmed from the current product datasheet, as these parameters may vary by lot and expression system.

Storage, Reconstitution, and Handling Guidelines

Proper storage and handling of Recombinant Human GOLGA8B Protein are critical to preserving its biological activity and structural integrity. The following general guidelines apply to recombinant proteins of this class; always refer to the product-specific datasheet supplied with each lot for definitive instructions.

• Storage Temperature: Recombinant proteins are typically stored at −20 °C or −80 °C for long-term stability. Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
• Reconstitution: If supplied as a lyophilized powder, reconstitute in the buffer recommended on the datasheet (commonly sterile PBS or a carrier-protein-containing buffer such as 0.1% BSA in PBS). Allow the vial to equilibrate to room temperature before opening.
• Aliquoting: Upon first thaw or reconstitution, prepare single-use aliquots to minimize freeze–thaw cycles and maintain consistency across experiments.
• Protein Stability: Addition of a carrier protein (e.g., 0.1–0.5% BSA or HSA) to the working solution may improve stability during storage and handling.
• Contamination Prevention: Use sterile, low-binding tubes and pipette tips. Work in a clean environment to avoid proteolytic degradation or microbial contamination.

Disclaimer: Specific buffer composition, concentration, and recommended reconstitution volumes are provided on the product datasheet. Always consult that document as the primary source of handling instructions.

Experimental Applications

Recombinant Human GOLGA8B Protein is intended for research use and may be applicable in a range of biochemical and cell biological assays. Typical research applications for recombinant golgin-family proteins include, but are not limited to:

• Protein–Protein Interaction Studies: Recombinant GOLGA8B can be used in pull-down assays, co-immunoprecipitation setups, or surface plasmon resonance (SPR) experiments to identify and characterize interaction partners within the Golgi trafficking machinery.
• Antibody Validation and Immunoassays: The purified recombinant protein serves as a positive control or coating antigen in ELISA, Western blot validation, and immunofluorescence antibody testing.
• Structural and Biophysical Studies: Recombinant golgins with their characteristic coiled-coil domains are amenable to biophysical characterization techniques such as circular dichroism (CD) spectroscopy and analytical ultracentrifugation.
• Functional Reconstitution Assays: Recombinant GOLGA8B may be used to reconstitute vesicle tethering events in cell-free systems, supporting mechanistic studies of Golgi membrane trafficking.
• Biomarker Research: Golgi proteins including GOLGA8B have been explored as potential biomarkers in certain disease contexts; the recombinant protein can be used to develop or validate detection assays.

Optimal assay conditions, working concentrations, and compatibility with specific experimental formats should be empirically determined and referenced against the product datasheet.

Quality, Purity, and Regulatory Considerations

Aladdin Scientific's Recombinant Human GOLGA8B Protein (SKU: rp372894) is manufactured and quality-controlled to meet the standards expected of research-grade recombinant proteins. Key quality considerations include:

• Purity: Recombinant proteins in this product line are typically assessed by SDS-PAGE and/or HPLC. Specific purity data are provided on the Certificate of Analysis (CoA) accompanying each lot.
• Endotoxin Testing: Endotoxin levels are measured (commonly by LAL assay) and reported on the CoA; low endotoxin formats are important for cell-based assays sensitive to LPS contamination.
• Lot-to-Lot Consistency: Each production lot is tested against defined release criteria. Researchers are encouraged to request the CoA for each received lot to verify compliance.
• Research Use Only: This product is supplied for research purposes only. It is not approved for, and must not be used in, diagnostic, therapeutic, or clinical procedures in humans or animals.
• Regulatory Compliance: Procurement teams should verify import/export requirements and any applicable institutional biosafety committee (IBC) regulations prior to ordering recombinant protein reagents.

For lot-specific quality data including purity percentage, activity, and endotoxin levels, consult the CoA provided with your shipment or contact Aladdin Scientific customer support.