Introduction to GOLGA8S and the Golgin Family

GOLGA8S (Golgi Autoantigen, Golgin Subfamily A, Member 8S) is a member of the golgin family of coiled-coil proteins that are associated with the Golgi apparatus. Golgins play essential structural and functional roles in maintaining Golgi morphology and facilitating intracellular vesicle tethering and membrane trafficking. The GOLGA8 gene family, located within a segmentally duplicated region of human chromosome 15, includes multiple paralogous members, of which GOLGA8S is one. These proteins are characterized by extended coiled-coil domains that enable protein–protein interactions critical for Golgi stack organization.

As an integral component of the Golgi apparatus proteome, GOLGA8S and its paralogs are of interest in studies ranging from basic cell biology to the investigation of chromosomal instability syndromes associated with segmental duplications on chromosome 15q.

Biochemical Properties of Recombinant Human GOLGA8S Protein

Recombinant Human GOLGA8S Protein (rp377481) is produced using recombinant expression technology to deliver a consistent, research-grade protein for use in laboratory investigations. The recombinant format ensures batch-to-batch reproducibility, which is critical for quantitative assays and comparative studies.

Key biochemical features characteristic of golgin-family proteins include extended alpha-helical coiled-coil regions that promote dimerization and interaction with Rab GTPases and SNARE proteins involved in vesicular trafficking. Researchers should consult the product datasheet for confirmed purity, molecular weight, formulation buffer, storage conditions, and recommended handling procedures specific to this lot.

Note: Detailed specifications such as exact molecular weight, purity percentage, endotoxin level, and host expression system for rp377481 should be verified against the current Aladdin Scientific product datasheet, as these parameters are lot-dependent and subject to update.

Research Applications

Recombinant Human GOLGA8S Protein is intended for research use only and is applicable across several experimental contexts in cell biology and biochemistry:

• Protein–protein interaction studies: GOLGA8S can be used as bait or prey in pull-down assays and co-immunoprecipitation experiments to identify binding partners within the Golgi trafficking machinery.
• Antibody validation and ELISA standards: Recombinant GOLGA8S serves as a positive control antigen for validating anti-GOLGA8S antibodies or as a standard in quantitative immunoassays.
• Structural and biophysical studies: The coiled-coil architecture of GOLGA8S makes it a suitable subject for circular dichroism (CD) spectroscopy, analytical ultracentrifugation, and other biophysical characterization methods.
• Functional reconstitution assays: Researchers studying Golgi membrane tethering or vesicle fusion may incorporate recombinant GOLGA8S into cell-free reconstitution systems.

All applications should be optimized by the end user for their specific experimental conditions.

Storage, Handling, and Quality Assurance

Proper storage and handling of Recombinant Human GOLGA8S Protein are essential to maintain protein integrity and biological activity. General best practices for recombinant proteins include:

• Store the protein at –20 °C or –80 °C upon receipt, as specified in the product datasheet.
• Avoid repeated freeze–thaw cycles; aliquot the protein into single-use volumes before freezing.
• Reconstitute lyophilized protein in the recommended buffer, allowing sufficient time for complete solubilization at the specified temperature.
• Centrifuge briefly before use to remove any particulates.

Aladdin Scientific's recombinant proteins undergo quality control testing to confirm identity and purity. Researchers should request the Certificate of Analysis (CoA) for lot-specific data including SDS-PAGE purity and additional QC metrics. This product is for research use only and is not intended for diagnostic, therapeutic, or veterinary use.