Introduction to GOLGA8T and the Golgin Family

GOLGA8T (Golgi Autoantigen, Golgin Subfamily A, Member 8T) is a member of the golgin family of coiled-coil proteins that are predominantly localized at the Golgi apparatus. Golgins play essential structural roles in maintaining Golgi morphology, facilitating vesicle tethering, and coordinating intracellular membrane trafficking pathways. The GOLGA8 gene cluster, located on human chromosome 15, has undergone significant copy-number variation and segmental duplication during primate evolution, giving rise to multiple paralogous members including GOLGA8T.

Although the precise molecular function of GOLGA8T remains under active investigation, its structural features — including characteristic coiled-coil domains — suggest involvement in Golgi ribbon formation and the regulation of cargo sorting at the trans-Golgi network. Recombinant production of GOLGA8T enables researchers to probe these functions through biochemical assays, binding studies, and structural analyses.

Protein Production and Biochemical Specifications

Recombinant Human GOLGA8T Protein (rp377482) is produced using standard recombinant DNA technology. The coding sequence of human GOLGA8T is expressed in a suitable host expression system, followed by downstream purification steps designed to yield a product of sufficient purity for use in demanding research applications. Recombinant golgins are typically produced as soluble proteins or with solubility-enhancing fusion tags given the extended coiled-coil nature of the polypeptide chain.

Researchers should consult the product datasheet for Aladdin Scientific SKU rp377482 for confirmed specifications including molecular weight, purity grade, formulation buffer, storage conditions, and recommended working concentrations. As with all recombinant proteins, optimal performance in specific assays may require empirical titration by the end user.

Research Applications

Recombinant Human GOLGA8T Protein is suitable for a range of in vitro research applications. Key areas of use include:

• Protein–protein interaction studies: Golgins serve as scaffolds that recruit Rab GTPases and other trafficking regulators; recombinant GOLGA8T can be used in pull-down assays or surface plasmon resonance experiments to identify binding partners.
• Antibody validation: Recombinant protein serves as a positive control antigen for validating anti-GOLGA8T antibodies used in Western blot, ELISA, or immunofluorescence workflows.
• Structural biology: Purified recombinant GOLGA8T can support X-ray crystallography, cryo-EM, or small-angle X-ray scattering studies aimed at resolving the coiled-coil architecture.
• Functional biochemical assays: The protein may be used to investigate membrane tethering activity and Golgi biogenesis in cell-free reconstitution systems.

Researchers investigating chromosomal instability or copy-number variation syndromes associated with the 15q13 region may also find recombinant GOLGA8T useful as a reference reagent.

Storage, Handling, and Quality Considerations

Proper handling of Recombinant Human GOLGA8T Protein is critical to maintaining activity and integrity. General best practices for recombinant proteins include:

• Store the protein at the temperature specified in the product datasheet (typically −20 °C or −80 °C for long-term storage).
• Avoid repeated freeze–thaw cycles; aliquot the protein upon first use to preserve activity.
• Keep the protein on ice during experimental setup to minimize degradation.
• Centrifuge the vial briefly before opening to collect contents at the bottom.

For lot-specific purity data, SDS-PAGE validation results, and endotoxin levels, refer to the Certificate of Analysis (CoA) available for SKU rp377482 through Aladdin Scientific. Researchers requiring additional technical support can contact Aladdin Scientific's technical services team.