What Is GOSR2? Biological Background and Function

GOSR2, also known as Membrin or GS27, is a member of the SNARE (Soluble NSF Attachment Protein Receptor) superfamily and belongs to the syntaxin family of proteins. It is a Golgi-resident integral membrane protein that plays a central role in the docking and fusion of transport vesicles at the Golgi apparatus.

GOSR2 functions as a t-SNARE (target-SNARE) on Golgi membranes, forming complexes with other SNARE proteins to drive membrane fusion events required for anterograde vesicular trafficking—specifically in ER-to-Golgi and intra-Golgi transport steps. This process is fundamental to the correct sorting and delivery of secretory and membrane proteins throughout the cell.

The protein is ubiquitously expressed across human tissues, consistent with its housekeeping role in maintaining Golgi structure and function. Disruption of GOSR2 function has been linked to defects in glycosylation and protein secretion, and mutations in the GOSR2 gene have been associated with progressive myoclonus epilepsy, underscoring its physiological importance.

Recombinant Human GOSR2 Protein: Production and Structural Features

Recombinant Human GOSR2 Protein is produced using established recombinant expression systems to yield a well-characterized, research-grade protein suitable for a variety of in vitro applications. Recombinant production ensures lot-to-lot consistency, defined purity, and freedom from animal-derived contaminants that may be present in tissue-extracted material.

The native GOSR2 protein contains a characteristic SNARE domain responsible for mediating protein-protein interactions during SNARE complex assembly, as well as a C-terminal transmembrane anchor that tethers the protein to Golgi membranes. Recombinant constructs may be produced as truncated, soluble forms (lacking the transmembrane domain) to facilitate expression and purification while retaining key functional and immunological epitopes.

Researchers should consult the specific product datasheet for Aladdin Scientific SKU rp365532 for confirmed details on the expression host, tag configuration, molecular weight, purity grade, formulation buffer, and recommended storage conditions.

Research Applications of Recombinant Human GOSR2 Protein

Recombinant Human GOSR2 Protein is a versatile reagent supporting multiple research disciplines focused on membrane trafficking, Golgi biology, and related disease mechanisms. Key applications include:

• SNARE Complex Assembly Studies: GOSR2 can be used in pull-down and co-immunoprecipitation assays to map its interactions with cognate SNARE partners (e.g., syntaxin 5, Ykt6, Bet1) and to characterize the stoichiometry and specificity of Golgi SNARE complexes.
• Antibody Validation and Immunoassay Development: Recombinant GOSR2 serves as a positive control antigen for Western blot, ELISA, and dot-blot assays, enabling researchers to validate anti-GOSR2 antibodies and establish assay sensitivity.
• Structural and Biophysical Studies: The purified protein can be used in biophysical characterization experiments such as circular dichroism (CD), surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC) to study folding, stability, and binding thermodynamics.
• Disease Mechanism Research: Given the association of GOSR2 mutations with progressive myoclonus epilepsy (PME), recombinant GOSR2 can support functional studies comparing wild-type and mutant protein behavior.
• Cell-Free Fusion Assays: Recombinant SNARE proteins including GOSR2 can be reconstituted into proteoliposomes for in vitro membrane fusion assays, a powerful approach to dissecting the molecular mechanism of vesicular transport.

Handling, Storage, and Quality Considerations

Proper handling and storage of Recombinant Human GOSR2 Protein are essential to maintain protein integrity and experimental reproducibility. General best practices for recombinant proteins apply:

• Storage: Store at the temperature specified on the product datasheet (typically −20 °C or −80 °C for long-term storage). Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze-thaw cycles.
• Carrier Protein: If the protein is supplied at low concentration, addition of a carrier protein such as BSA may be recommended to prevent adsorption to tube surfaces—consult the datasheet for guidance.
• Reconstitution: If supplied lyophilized, reconstitute in the recommended buffer as specified by the datasheet, and allow the protein to equilibrate fully before use.
• Quality Control: Aladdin Scientific's recombinant proteins undergo quality control testing; consult the Certificate of Analysis (CoA) for lot-specific purity, endotoxin levels, and biological activity data.

Always refer to the specific product datasheet for SKU rp365532 for authoritative handling instructions.