What Is GPANK1?

GPANK1 (G-Patch Domain and Ankyrin Repeats 1), also known as GAN-binding protein or ANKRD52 in some literature contexts, is a human protein characterized by two distinctive structural motifs: a G-patch domain and ankyrin repeat units. The G-patch domain is a conserved glycine-rich motif found in a variety of RNA-associated proteins, while ankyrin repeats mediate protein–protein interactions across diverse biological contexts.

GPANK1 is encoded by the GPANK1 gene in the human genome. The protein is thought to participate in cellular regulatory networks, potentially involving RNA processing and protein scaffolding functions, though its precise molecular roles remain an active area of investigation. Research into GPANK1 and its interaction partners may yield insights into cellular homeostasis and disease-associated pathways.

Biochemical Properties of Recombinant Human GPANK1 Protein

The Recombinant Human GPANK1 Protein (rp375061) available from Aladdin Scientific is produced using recombinant expression systems to deliver a well-defined, research-grade protein. Key structural features include:

• G-patch domain: A glycine-rich motif implicated in RNA binding and regulation of RNA helicases.
• Ankyrin repeats: Modular protein interaction domains that facilitate binding to diverse protein partners and contribute to the protein's scaffolding role.

As with all recombinant proteins, purity, host expression system, tag configuration, and storage conditions are critical parameters. Researchers are advised to consult the product datasheet (absea#PP-8794) for confirmed specifications including molecular weight, purity grade, formulation buffer, and recommended storage temperature, as these details are subject to lot-specific variation.

Research Applications

Recombinant Human GPANK1 Protein supports a range of in vitro and biochemical research applications:

• Protein–protein interaction studies: The ankyrin repeat domains make GPANK1 a useful tool for pull-down assays and co-immunoprecipitation experiments aimed at mapping its interactome.
• Antibody development and validation: Recombinant GPANK1 can serve as an immunogen for raising polyclonal or monoclonal antibodies, or as a standard for ELISA and Western blot assay validation.
• Structural biology: Purified recombinant protein is suitable for crystallography or cryo-EM studies aimed at elucidating domain architecture.
• Functional assays: Investigating the role of GPANK1 in RNA processing pathways or protein complex assembly in cell-free systems.
• Biomarker research: As a reference standard in assay development where GPANK1 expression or abundance is under investigation.

Always confirm suitability for a specific application by consulting the product datasheet and performing appropriate validation experiments in your laboratory.

Handling, Storage, and Quality Considerations

Proper handling of recombinant proteins is essential to maintain biological activity and ensure reproducible results. General best-practice guidelines for Recombinant Human GPANK1 Protein include:

• Storage: Refer to the product datasheet for the recommended storage temperature (typically −20 °C or −80 °C for long-term storage). Avoid repeated freeze–thaw cycles, which can reduce protein integrity.
• Reconstitution: If supplied in lyophilized form, reconstitute in the recommended buffer according to the datasheet instructions. Allow the vial to equilibrate to room temperature before opening.
• Aliquoting: Prepare single-use aliquots upon first thaw to minimize degradation from repeated freeze–thaw.
• Quality assessment: Confirm purity and identity by SDS-PAGE and, where applicable, Western blot using a validated anti-GPANK1 antibody before use in critical experiments.

For lot-specific data including purity, endotoxin levels, and activity data, consult the Certificate of Analysis (CoA) provided with each shipment or available upon request from Aladdin Scientific.