Background: GPRIN2 and the GPRIN Protein Family

GPRIN2 (G Protein Regulated Inducer of Neurite Outgrowth 2) is a member of the GPRIN protein family, which includes GPRIN1, GPRIN2, and GPRIN3. These proteins are characterized by their ability to interact with activated G-protein alpha subunits, particularly Gα_o and Gα_i, playing a regulatory role in G-protein-coupled receptor (GPCR) signaling cascades.

GPRIN2 is predominantly expressed in the nervous system and has been associated with the regulation of neurite outgrowth, a critical process in neuronal development, axonal regeneration, and synaptic plasticity. As a modulator of G-protein signaling, GPRIN2 is considered relevant to studies of neurological function and may have implications in the context of neurological and psychiatric disorders.

The protein contains conserved structural motifs that mediate protein-protein interactions within the G-protein signaling network. Understanding GPRIN2's molecular interactions offers valuable insight into mechanisms governing neuronal connectivity and intracellular signal propagation.

Biochemical Properties of Recombinant Human GPRIN2 Protein

Recombinant Human GPRIN2 Protein (SKU: rp375066) is produced using recombinant DNA technology, enabling consistent batch-to-batch quality suitable for demanding research applications. Recombinant production systems allow researchers to obtain sufficient quantities of the protein in a defined, reproducible format, which is essential for biochemical and cell-based experiments.

Researchers should consult the official Aladdin Scientific product datasheet for rp375066 for confirmed specifications, including molecular weight, expression host, tag information, purity grade, endotoxin levels, and recommended storage conditions. These parameters are critical for experimental design and reproducibility.

As with other recombinant proteins in Aladdin Scientific's portfolio, GPRIN2 protein is intended for in vitro research use only and is not approved for therapeutic, diagnostic, or clinical applications.

Research Applications of GPRIN2 Recombinant Protein

Recombinant Human GPRIN2 Protein is suited for a range of in vitro research applications relevant to neuroscience and cell biology:

• Protein-Protein Interaction Studies: GPRIN2 can be used in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) studies to map its interactions with G-protein subunits and other binding partners.
• Biochemical Assays: The recombinant protein serves as a substrate or binding partner in enzyme-linked immunosorbent assays (ELISA), Western blot validation, and functional binding assays.
• Antibody Development and Validation: Purified recombinant GPRIN2 protein is a reliable antigen for raising and validating antibodies targeting endogenous GPRIN2 in experimental models.
• Signal Transduction Research: GPRIN2 can be employed to investigate its modulatory role in GPCR signaling, Gα subunit activity, and downstream effector regulation in neuronal and non-neuronal cell contexts.
• Neurite Outgrowth Studies: Researchers studying axonal growth, neuronal differentiation, or regeneration may use GPRIN2 protein to explore its functional contributions in vitro.

Handling, Storage, and Quality Considerations

To ensure optimal performance of Recombinant Human GPRIN2 Protein in experimental settings, researchers should adhere to the following general best practices, in addition to the specific guidelines provided in the Aladdin Scientific product datasheet:

• Storage: Recombinant proteins are typically stored at −20°C or −80°C in single-use aliquots to minimize freeze-thaw cycles. Confirm exact conditions from the product datasheet for rp375066.
• Reconstitution: If supplied lyophilized, reconstitute the protein in the recommended buffer as specified in the datasheet. Allow the protein to equilibrate to room temperature before use.
• Avoid Repeated Freeze-Thaw Cycles: Aliquot the protein upon first use to preserve activity and prevent aggregation.
• Endotoxin Levels: For cell-based applications, verify endotoxin specifications to ensure results are not confounded by lipopolysaccharide (LPS) contamination.
• Quality Control: Aladdin Scientific applies rigorous quality control testing to recombinant proteins. Specific QC data, including purity by SDS-PAGE and biological activity, should be reviewed in the official certificate of analysis (CoA).

Ordering Information

Recombinant Human GPRIN2 Protein is available from Aladdin Scientific under the following catalog information:

• Product Name: Recombinant Human GPRIN2 Protein
• SKU: rp375066
• Supplier Reference: absea#PP-8799

For current pricing, available quantities, and bulk order inquiries, please contact Aladdin Scientific directly or visit the product page on the Aladdin Scientific website. Aladdin Scientific's technical support team is available to assist with product selection, application guidance, and custom requirements.