What Is GPSM2 and Why Is It Important in Research?

GPSM2, also known as LGN (Leu-Gly-Asn repeat-enriched protein) or AGS3L, is a highly conserved scaffolding protein that plays a central role in regulating G-protein signaling independently of G-protein-coupled receptors (GPCRs). It contains multiple GoLoco/GPR motifs at its C-terminus, which interact with Gαi subunits, and tetratricopeptide repeat (TPR) domains at its N-terminus that bind NuMA (Nuclear Mitotic Apparatus protein) and other partners.

GPSM2 is critically involved in the orientation of the mitotic spindle during cell division, a process essential for asymmetric cell division and the maintenance of tissue architecture. Dysregulation of GPSM2 has been linked to hearing loss (Chudley-McCullough syndrome), defects in cortical neurogenesis, and aberrant cell proliferation in certain cancers. These diverse roles make Recombinant Human GPSM2 Protein a valuable tool for researchers studying cell polarity, neuronal development, and oncology.

Frequently Asked Questions About Product Specifications

Q: What host expression system is used to produce this recombinant protein?
A: The specific expression system for Recombinant Human GPSM2 Protein (rp372287) should be confirmed in the product datasheet. Common systems for this type of scaffolding protein include E. coli, baculovirus/insect cell, or mammalian expression. Please refer to the official product page or contact Aladdin Scientific technical support for the confirmed expression system.

Q: What is the purity of rp372287?
A: Purity specifications are provided in the product-specific datasheet. For recombinant proteins intended for research use, purity is typically assessed by SDS-PAGE. Please consult the product datasheet or contact our technical team for lot-specific certificate of analysis (CoA) data.

Q: Is the protein supplied with a tag, and if so, which tag?
A: Tag information (e.g., His-tag, GST-tag, or tag-free) is detailed in the product datasheet for rp372287. Knowing the tag is important for downstream applications such as pull-down assays or detection with anti-tag antibodies. Contact Aladdin Scientific for datasheet access.

Storage, Handling, and Stability Guidelines

Proper storage and handling of Recombinant Human GPSM2 Protein are essential to preserve its biological activity and structural integrity. While specific conditions should always be verified against the product datasheet, the following general best practices apply to most recombinant proteins of this class:

• Temperature: Store at –80°C for long-term storage. Avoid repeated freeze-thaw cycles, as these can lead to protein aggregation and loss of activity.
• Aliquoting: Upon receipt, it is strongly recommended to prepare single-use aliquots before freezing to minimize freeze-thaw damage.
• Working solution: Once thawed, keep on ice and use within the recommended timeframe specified in the datasheet.
• Buffer compatibility: Check the supplied buffer formulation for compatibility with your downstream assay. Carrier proteins such as BSA or glycerol may be present as stabilizers.

Always refer to the lot-specific CoA and product datasheet for confirmed storage conditions for rp372287.

Recommended Research Applications

Recombinant Human GPSM2 Protein is suitable for a range of in vitro biochemical and cell biological research applications. Typical applications for recombinant proteins of this class include:

• Protein–protein interaction studies: GPSM2 interacts with Gαi subunits and NuMA; recombinant protein can be used in pull-down, co-immunoprecipitation validation, or surface plasmon resonance (SPR) assays.
• Biochemical activity assays: Investigating GoLoco motif-mediated GDI (guanine nucleotide dissociation inhibitor) activity on Gαi proteins.
• Structural studies: As a component in crystallography or cryo-EM studies of the LGN–NuMA–dynein complex.
• Antibody validation: Used as a positive control antigen in Western blot or ELISA to validate anti-GPSM2 antibodies.
• Drug discovery: Screening for small molecules that disrupt GPSM2–Gαi or GPSM2–NuMA interactions relevant to cancer and hearing disorder research.

Please verify application suitability with the product datasheet and consider requesting a technical consultation from Aladdin Scientific prior to experimental design.

Ordering, Quality, and Technical Support

How do I order Recombinant Human GPSM2 Protein?
Recombinant Human GPSM2 Protein is available from Aladdin Scientific under SKU rp372287. Orders can be placed directly through the Aladdin Scientific website or by contacting your regional sales representative. For bulk quantities or custom formulations, please reach out to our sales team for a custom quotation.

What quality documentation is available?
A Certificate of Analysis (CoA) is available for each production lot and can be requested at the time of order or downloaded from the product page using the lot number. The CoA includes purity assessment, formulation details, and quality control test results.

How can I get technical support?
Aladdin Scientific's technical support team is available to assist with questions about experimental design, buffer compatibility, and application optimization. Contact us via the website's support portal, email, or phone. We also recommend consulting published literature on GPSM2 biology to inform your experimental approach.