What Is GPX5 and Why Is It Important?

GPX5 (Glutathione Peroxidase 5) is a member of the glutathione peroxidase superfamily. Unlike classical selenium-containing GPX family members, GPX5 is a non-selenocysteine glutathione peroxidase that is expressed predominantly in the epididymis. It plays a critical role in protecting sperm from oxidative stress during epididymal transit, a period when spermatozoa are particularly vulnerable to reactive oxygen species (ROS)-mediated lipid peroxidation and DNA damage.

GPX5 is secreted into the epididymal lumen and associates with the sperm surface, contributing to the maintenance of sperm membrane integrity and fertility. Research into GPX5 has significant implications for understanding male infertility, sperm quality, and antioxidant defense mechanisms in the male reproductive tract.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human GPX5 Protein (SKU: rp368187) is produced to research-grade standards. The following specifications are typical for recombinant proteins in this category; researchers should consult the official product datasheet for confirmed lot-specific values:

• Expression System: Typically expressed in a heterologous system (e.g., E. coli or mammalian cells); refer to the current datasheet for confirmed expression host.
• Purity: Generally >90% as determined by SDS-PAGE; lot-specific purity data available on the Certificate of Analysis (CoA).
• Formulation: Supplied in a buffer optimized for protein stability; exact formulation detailed in the product datasheet.
• Endotoxin: Tested and reported on the CoA; suitable for cell-based assays when endotoxin levels meet experimental requirements.

Always download the most current datasheet and CoA from the Aladdin Scientific product page before use.

Storage, Handling, and Reconstitution

Proper storage and handling are essential to maintain the activity and integrity of Recombinant Human GPX5 Protein. General best-practice guidelines for recombinant proteins include:

• Short-term storage: Store at 4°C for up to 1–2 weeks if the protein will be used promptly.
• Long-term storage: Aliquot and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can denature the protein and reduce activity.
• Reconstitution: If supplied lyophilized, reconstitute in sterile deionized water or the buffer specified in the datasheet. Allow the vial to equilibrate to room temperature before opening.
• Carrier protein: Addition of a carrier protein (e.g., 0.1% BSA) to the working solution may improve stability during storage.

Refer to the product-specific datasheet for confirmed storage conditions and reconstitution instructions, as these may vary by lot and formulation.

Research Applications of Recombinant Human GPX5 Protein

Recombinant Human GPX5 Protein is a versatile tool for multiple research applications, including:

• Biochemical assays: Used as a standard or positive control in ELISA and Western blot experiments targeting GPX5.
• Functional studies: Investigation of GPX5 antioxidant activity and its role in protecting lipid membranes from peroxidative damage.
• Male fertility research: Studies examining the relationship between epididymal protein secretion, oxidative stress, and sperm quality or motility.
• Protein-protein interaction studies: Exploring GPX5 binding partners on the sperm surface or within the epididymal microenvironment.
• Drug discovery: Screening compounds that modulate oxidative stress pathways in the male reproductive tract.
• Antibody validation: Serving as an antigen for antibody specificity testing and immunoassay development.

Ordering, Shipping, and Support

Recombinant Human GPX5 Protein (SKU: rp368187) is available for order through the Aladdin Scientific website or by contacting your regional sales representative. Key ordering and logistics information:

• Ordering: Place orders online at the Aladdin Scientific product page for rp368187, or contact customer service for bulk pricing and custom requirements.
• Shipping: Recombinant proteins are shipped on dry ice to maintain cold-chain integrity. Confirm shipping conditions with Aladdin Scientific at the time of order for your region.
• Lead time: Standard lead times apply; contact sales for current availability and expedited options.
• Technical support: For protocol guidance, troubleshooting, or application-specific questions, contact Aladdin Scientific's technical support team via the website or by email.
• Returns and refunds: Please review Aladdin Scientific's returns and refund policy on the website before placing your order.