What Is GRIA3 and Why Is It Important in Research?

GRIA3 (Glutamate Ionotropic Receptor AMPA Type Subunit 3), also known as GluA3 or GluR-C, encodes a key subunit of AMPA-type ionotropic glutamate receptors. AMPA receptors are tetrameric ligand-gated ion channels that mediate the majority of fast excitatory neurotransmission in the mammalian central nervous system. The GRIA3 subunit contributes to receptor diversity, calcium permeability, and synaptic plasticity depending on its RNA editing status and associated auxiliary subunits.

GRIA3 has been linked to several neurological and psychiatric conditions, including X-linked intellectual disability (MRX94), schizophrenia, and epilepsy, making it a clinically relevant research target. Recombinant Human GRIA3 Protein enables researchers to investigate receptor-ligand interactions, subunit assembly, and the functional consequences of disease-associated mutations under controlled in vitro conditions.

Recommended Applications for Recombinant Human GRIA3 Protein

Recombinant Human GRIA3 Protein (rp368058) is suitable for a range of in vitro research applications, including:

• Biochemical binding assays: Characterizing small-molecule or peptide interactions with the GRIA3 ligand-binding domain.
• Antibody validation: Serving as a positive control antigen for ELISA, Western blot, and immunoprecipitation assays targeting GRIA3.
• Protein–protein interaction studies: Investigating interactions between GRIA3 and auxiliary subunits such as TARPs or cornichon proteins.
• Structural and biophysical research: Supporting crystallography, cryo-EM sample preparation, or SPR/BLI kinetic studies.
• Drug discovery: Screening compound libraries for modulators of GRIA3-containing AMPA receptors.

Researchers should validate the protein in their specific assay format prior to use in critical experiments.

Storage, Handling, and Stability of Recombinant Human GRIA3 Protein

Proper storage and handling are essential to maintain the activity and integrity of Recombinant Human GRIA3 Protein. General best-practice guidelines for recombinant proteins apply:

• Short-term storage (≤1 month): Store at 4 °C in the supplied buffer to minimize freeze-thaw cycles.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Carrier protein: If the protein is supplied at low concentration, consider adding a carrier protein such as BSA (0.1%) to prevent adsorption to tube surfaces.
• Handling: Keep on ice during experimental setup. Centrifuge briefly before opening to collect any condensate.

Always refer to the product datasheet for lot-specific storage conditions, buffer composition, and recommended working concentrations. Contact Aladdin Scientific technical support if specific storage details are not available for your lot.

Quality, Purity, and Validation of rp368058

Aladdin Scientific's Recombinant Human GRIA3 Protein (rp368058) is produced to research-grade standards. Key quality considerations include:

• Purity: Recombinant proteins in this category are typically produced by affinity chromatography and assessed by SDS-PAGE and/or HPLC. Refer to the Certificate of Analysis (CoA) for lot-specific purity data.
• Identity confirmation: Protein identity is confirmed by mass spectrometry or N-terminal sequencing.
• Endotoxin testing: Endotoxin levels are measured by LAL assay; low endotoxin preparations are recommended for cell-based assays.
• Functional validation: Batch activity may be confirmed by ligand-binding assay or other functional tests as described in the product datasheet.

Researchers requiring specific purity grades or custom formulations should contact Aladdin Scientific for OEM or bulk options.

Ordering, Shipping, and Returns for GRIA3 Recombinant Protein

Recombinant Human GRIA3 Protein (rp368058) can be ordered directly through the Aladdin Scientific website or by contacting your regional sales representative. Key logistics information:

• Shipping: Cold-chain shipping (dry ice) is standard for recombinant proteins to preserve stability during transit. Shipping conditions are specified on the product page and CoA.
• Lead time: Standard lead times apply; contact Aladdin Scientific for current availability and estimated delivery windows.
• Returns and refunds: Due to the temperature-sensitive nature of recombinant proteins, returns are subject to Aladdin Scientific's standard returns policy. Please inspect packages upon receipt and report any damage or discrepancy within the specified notification window.
• Bulk and OEM orders: Available upon request. Contact technical sales for custom quantity pricing.

For procurement questions, pricing, or promotional information, please contact Aladdin Scientific customer service or your institutional purchasing office.