Recombinant Human GRID2IP Protein: Properties & Research Uses
Recombinant Human GRID2IP Protein: Properties & Research Uses
Recombinant Human GRID2IP Protein is a recombinantly expressed form of the glutamate receptor delta-2 interacting protein, a key synaptic scaffolding molecule that mediates anchoring of the GluD2 receptor at cerebellar parallel fiber–Purkinje cell synapses and supports downstream signaling relevant to motor learning and synapse formation research.
- Product Name
- Recombinant Human GRID2IP Protein
- SKU
- rp372753
- Also Known As
- GRID2 Interacting Protein; Delphilin
- Protein Origin
- Human (Homo sapiens)
- Primary Function
- Synaptic scaffolding; anchors GluD2 (GRID2) at cerebellar Purkinje cell synapses
- Research Applications
- Neuroscience, glutamate receptor biology, synaptic plasticity, cerebellum studies
What Is GRID2IP? Gene, Protein and Aliases
GRID2IP (Glutamate Receptor Ionotropic, Delta-2 Interacting Protein), also referred to as Delphilin, is encoded by the GRID2IP gene in humans. The protein functions as a postsynaptic scaffolding molecule that physically interacts with the C-terminal PDZ-binding motif of GluD2 (GRID2), the glutamate receptor delta-2 subunit. This interaction is critical for the stable localization of GluD2 at the postsynaptic density of parallel fiber–Purkinje cell synapses in the cerebellum.
GRID2IP belongs to a class of multi-domain scaffolding proteins and contains formin homology (FH) domains, which implicate it in actin cytoskeleton regulation at the synapse. Through these structural features, GRID2IP is thought to couple glutamate receptor positioning to cytoskeletal dynamics, influencing synapse morphology and stability.
Biological Function and Signaling Pathways
The primary biological role of GRID2IP is to anchor GluD2 at cerebellar synapses, ensuring receptor clustering and maintaining the fidelity of synaptic transmission. GluD2, despite belonging to the ionotropic glutamate receptor superfamily, is thought to play predominantly structural and trans-synaptic signaling roles rather than functioning as a classical ion channel under normal physiological conditions.
GRID2IP-mediated scaffolding supports the interaction between GluD2 and cerebellin-1 (Cbln1) at the presynaptic active zone via neurexin, forming a trans-synaptic complex that is essential for synapse formation and maintenance. Disruption of GRID2IP interactions may therefore impair cerebellar synapse integrity, long-term depression (LTD) at parallel fiber–Purkinje cell synapses, and motor coordination.
Additionally, the formin homology domains of GRID2IP suggest involvement in actin polymerization and dendritic spine morphology regulation, linking receptor scaffolding to broader cytoskeletal remodeling events during synaptic plasticity.
Research Applications of Recombinant Human GRID2IP Protein
Recombinant Human GRID2IP Protein (SKU: rp372753) is suitable for a range of in vitro research applications in neuroscience and cell biology:
- Protein–protein interaction studies: Recombinant GRID2IP can be used in pull-down assays, co-immunoprecipitation validation experiments, and surface plasmon resonance (SPR) studies to characterize its binding to GluD2 and other postsynaptic partners.
- Structural and biochemical assays: The purified protein supports circular dichroism, gel filtration, and other biophysical analyses to probe domain folding and stability.
- Antibody validation: Recombinant GRID2IP serves as a positive control antigen for validating antibodies targeting endogenous GRID2IP in western blotting, ELISA, and immunohistochemistry workflows.
- Functional assays: The protein may be applied in actin polymerization assays that leverage its formin homology domains, contributing to mechanistic studies of cytoskeletal dynamics at synapses.
- Drug discovery support: As a scaffolding component at cerebellar synapses, GRID2IP is a potential target-related reagent for screening compounds that modulate GluD2 receptor complexes.
Storage, Handling and Quality Considerations
Recombinant proteins such as GRID2IP should be handled under conditions that preserve biological activity and minimize aggregation. General best practices for recombinant protein reagents include:
- Store lyophilized protein at –20 °C or –80 °C upon receipt; avoid repeated freeze–thaw cycles once reconstituted.
- Reconstitute in the buffer recommended by the manufacturer, typically a physiological pH buffer supplemented with a carrier protein (e.g., BSA) or glycerol to enhance stability.
- Centrifuge briefly before opening the vial to ensure the material is at the bottom of the tube.
- Aliquot reconstituted protein to single-use volumes to minimize degradation.
For specific storage conditions, reconstitution volumes, concentration, purity grade, and host expression system details for rp372753, please refer to the official Aladdin Scientific product datasheet or contact technical support.
Frequently asked questions
What is the function of GRID2IP in the nervous system?
GRID2IP (also called Delphilin) acts as a postsynaptic scaffolding protein that anchors the GluD2 glutamate receptor (GRID2) at cerebellar parallel fiber–Purkinje cell synapses. It helps maintain synapse integrity and supports cerebellar-dependent processes such as motor learning and long-term synaptic depression (LTD).
What research applications is Recombinant Human GRID2IP Protein suitable for?
Recombinant Human GRID2IP Protein (rp372753) is suitable for protein–protein interaction assays (e.g., pull-down, SPR), antibody validation as a positive control antigen, biophysical characterization, actin polymerization assays utilizing its formin homology domains, and as a reagent supporting drug discovery programs targeting GluD2 receptor complexes.
How should Recombinant Human GRID2IP Protein be stored?
As a general guideline for recombinant proteins, lyophilized material should be stored at –20 °C or –80 °C and reconstituted according to the manufacturer's datasheet. Repeated freeze–thaw cycles of reconstituted protein should be avoided by preparing single-use aliquots. Refer to the Aladdin Scientific product datasheet for rp372753 for specific conditions.
What is the difference between GRID2IP and GRID2?
GRID2 (GluD2) is the glutamate receptor delta-2 subunit, an ionotropic glutamate receptor expressed predominantly in cerebellar Purkinje cells. GRID2IP is a distinct interacting protein (scaffolding molecule) that binds to the C-terminal domain of GRID2 and anchors it at the synapse. They are separate proteins encoded by different genes but function together within the same synaptic complex.
References
- Yuzaki M. (2003). The delta2 glutamate receptor: 10 years later. Neuroscience Research, 46(1), 11–22.
- Fukaya M. et al. (2005). Abundant distribution of TARP gamma-8 in synaptic and extrasynaptic surface of hippocampal neurons and its major role in AMPA receptor expression on spines and dendrites — referenced as contextual scaffolding comparison. (Placeholder: specific GRID2IP primary literature should be verified.)
- Matsuda K. and Yuzaki M. (2011). Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions. European Journal of Neuroscience, 33(8), 1447–1461.
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