What Is Recombinant Human GRM7 Protein?

GRM7 (also known as mGluR7) is a member of the metabotropic glutamate receptor family, specifically belonging to Group III metabotropic glutamate receptors and the Class C G protein-coupled receptor (GPCR) superfamily. It is predominantly expressed in the central nervous system (CNS), where it functions as a presynaptic autoreceptor that modulates glutamatergic and GABAergic neurotransmission by inhibiting adenylyl cyclase activity upon activation.

Recombinant Human GRM7 Protein (SKU: rp368111) from Aladdin Scientific is a research-grade protein produced through recombinant expression technology, designed to provide a reliable and reproducible tool for in vitro research applications including binding assays, functional studies, and structural investigations of this receptor.

Gene name: GRM7 | Also known as: mGluR7, GPRC1G, MGLUR7

Biological Significance and Research Applications

GRM7 plays a critical regulatory role in synaptic transmission throughout the brain and spinal cord. As a presynaptic inhibitory autoreceptor, it limits excessive neurotransmitter release, making it an important modulator of neuronal excitability. Dysregulation of GRM7 signaling has been implicated in multiple neurological and psychiatric conditions, including anxiety disorders, depression, schizophrenia, epilepsy, and chronic pain.

Recombinant Human GRM7 Protein is used in a range of research applications:

• Ligand binding and competition assays: Characterizing interactions between GRM7 and agonists, antagonists, or allosteric modulators.
• Drug discovery screening: Identifying novel small-molecule modulators targeting the GRM7 binding pocket for CNS therapeutic development.
• Structural biology: Supporting crystallography or cryo-EM studies aimed at resolving the receptor's three-dimensional architecture.
• Biochemical pathway studies: Investigating GRM7-mediated signal transduction cascades, including Gi/o protein coupling and downstream second messenger effects.
• Antibody validation: Serving as a positive control antigen for GRM7-specific antibody characterization.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human GRM7 Protein (rp368111) is manufactured and quality-controlled to meet the rigorous standards required for reliable research outcomes. Researchers should consult the official product datasheet for the most current and complete specification details, as formulation, purity grade, and format may be updated.

Key specification parameters to review on the datasheet include:

• Expression system: The recombinant expression host (e.g., mammalian, insect, or E. coli cells) impacts protein folding, post-translational modifications, and functional activity. Please verify on the product page.
• Purity: Assessed by SDS-PAGE and/or HPLC; target purity grade is indicated on the Certificate of Analysis (CoA).
• Formulation/Buffer: Supplied in a defined buffer system optimized for protein stability; refer to the CoA or datasheet.
• Endotoxin level: Tested to ensure suitability for cell-based assays; specific values are provided on the CoA.
• Molecular weight: Determined by the expressed construct; verify against the datasheet as tag additions may alter apparent molecular weight.

Each lot is accompanied by a Certificate of Analysis (CoA) documenting quality control testing results. Contact Aladdin Scientific technical support for lot-specific CoA requests.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GRM7 Protein are essential to preserve activity and ensure reproducible results across experiments. Always consult the product-specific datasheet for authoritative storage instructions, as conditions may vary by lot or formulation.

General best-practice guidelines for recombinant proteins:

• Long-term storage: Store at −70 °C or −80 °C to maximize shelf life. Avoid storage at −20 °C for extended periods, as freeze-thaw cycles and frost-free freezer temperature fluctuations can degrade protein integrity.
• Aliquoting: Upon receipt, prepare single-use aliquots to minimize repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Working stock: Short-term storage (up to 1 week) at 4 °C is acceptable for actively used material; do not leave at room temperature for prolonged periods.
• Carrier protein: If the protein is supplied without a stabilizing carrier, consider adding BSA (0.1% final concentration) to prevent adsorption to tube walls and improve stability.
• Avoid repeated freeze-thaw: Each freeze-thaw cycle can reduce protein activity; limit cycles to fewer than three wherever possible.

Note: The specific recommended storage conditions, expiry, and stability data for rp368111 are provided on the product datasheet and CoA. The guidelines above are general recommendations for recombinant proteins and should not supersede product-specific instructions.

Ordering, Technical Support, and Returns

Aladdin Scientific is committed to providing researchers with high-quality reagents backed by responsive technical and customer support.

How to order: Recombinant Human GRM7 Protein (rp368111) can be ordered directly through the Aladdin Scientific website. Add the product to your cart using the SKU rp368111, select the desired quantity, and proceed through the secure checkout. Institutional purchase orders are accepted; contact your regional sales representative for volume pricing or custom quotations.

Technical support: For application-specific guidance—such as recommended assay conditions, buffer compatibility, or troubleshooting experimental results—contact the Aladdin Scientific technical support team via the website inquiry form or by email. Technical specialists are available to assist with protocol development and product selection.

Returns and refunds: Products are subject to Aladdin Scientific's standard returns and refunds policy. Because biological reagents have specific handling requirements, eligibility for return is assessed on a case-by-case basis. Please contact customer service within the applicable timeframe after receipt if you experience quality concerns. A CoA and documentation of the issue may be required to process a claim.

Shipping and delivery: Recombinant proteins are shipped on dry ice to maintain cold-chain integrity. Delivery timelines vary by destination; confirm lead times and shipping options at the time of order placement.