What Is Hormone Sensitive Lipase and Why Does It Matter?

Hormone Sensitive Lipase (HSL) is a multifunctional intracellular neutral lipase expressed predominantly in adipose tissue, steroidogenic tissues, heart, skeletal muscle, and pancreatic beta cells. It catalyzes the hydrolysis of diacylglycerols, triacylglycerols, cholesteryl esters, and retinyl esters, making it a central enzyme in lipid mobilization and energy homeostasis.

HSL activity is tightly regulated by hormonal signals: catecholamines activate HSL via the cAMP–protein kinase A (PKA) signaling axis, leading to phosphorylation and translocation to lipid droplets, while insulin suppresses its activity. This hormonal control positions HSL as a critical node linking endocrine signaling to fatty acid release and systemic energy balance.

Dysregulation of HSL has been associated with metabolic disorders including obesity, type 2 diabetes, dyslipidemia, and cardiovascular disease, underscoring its importance as a research and potential therapeutic target.

Recombinant Human HSL Protein: Production and Biochemical Properties

Recombinant Human Hormone Sensitive Lipase Protein (SKU rp377005) is produced using recombinant expression systems to deliver a well-defined, research-grade enzyme. Recombinant production ensures batch-to-batch consistency, defined purity, and the absence of endogenous lipase contaminants that could confound experimental results.

The recombinant protein retains the catalytic serine residue within the canonical GXSXG lipase motif, which is essential for ester bond hydrolysis. Post-translational regulatory phosphorylation sites are conserved in the human sequence, making the recombinant protein suitable for studies examining kinase-dependent activation and protein–protein interactions with regulatory partners such as perilipin and ABHD5.

Researchers should consult the product datasheet (absea#PP-10531) for lot-specific information on purity, formulation buffer, reconstitution conditions, and activity data before experimental use.

Research Applications of Recombinant Human HSL Protein

Recombinant Human HSL Protein supports a broad range of in vitro research applications relevant to metabolic biology, drug discovery, and biochemical characterization:

• Enzyme activity assays: Measure HSL-mediated hydrolysis of fluorogenic or radiolabeled lipid substrates to determine kinetic parameters (Km, Vmax) and screen inhibitor or activator compounds.
• Drug discovery and inhibitor screening: HSL is an established target for antiobesity and antidiabetic drug programs. The recombinant protein enables high-throughput screening of small-molecule libraries.
• Protein interaction studies: Investigate HSL binding to regulatory proteins such as perilipins, 14-3-3 proteins, and lipid droplet-associated factors using pull-down assays, surface plasmon resonance, or co-immunoprecipitation.
• Phosphorylation and signaling studies: Use the recombinant protein as a substrate for PKA or AMPK in in vitro phosphorylation assays to map regulatory sites.
• Antibody validation: Serve as a positive control antigen for Western blotting, ELISA, or immunofluorescence assay development targeting HSL.

Handling, Storage, and Reconstitution Recommendations

Proper handling of recombinant lipase proteins is essential to preserve enzymatic activity and avoid experimental variability. The following general best practices apply; always verify against the specific lot datasheet for rp377005 (absea#PP-10531):

• Storage: Recombinant proteins should typically be stored at −80 °C for long-term stability. Avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first reconstitution.
• Reconstitution: Reconstitute lyophilized protein in the buffer specified on the datasheet, typically sterile water or a carrier protein-containing buffer (e.g., 0.1% BSA in PBS), to achieve a working stock concentration.
• Handling: Work on ice to minimize thermal denaturation. Use low-binding microcentrifuge tubes to reduce protein adsorption to tube walls.
• Activity verification: Include a positive control of known activity in every assay run to confirm protein integrity and batch performance.

Do not use the protein beyond the expiry date indicated on the product label. Contact Aladdin Scientific technical support for guidance on specific experimental formats.

Ordering and Technical Support from Aladdin Scientific

Recombinant Human Hormone Sensitive Lipase Protein is available from Aladdin Scientific under SKU rp377005. This product is referenced under supplier catalog number absea#PP-10531.

To order or request additional technical documentation including the full datasheet, certificate of analysis (CoA), or safety data sheet (SDS), contact Aladdin Scientific customer service or visit the product page directly on the Aladdin Scientific website.

Aladdin Scientific's technical team is available to assist with application recommendations, cross-reactivity queries, and custom requirements. Bulk and institutional pricing inquiries are welcomed through the procurement channel.