What Is HOXD8 and Why Is It Important?

HOXD8 is a member of the HOX family of homeodomain-containing transcription factors, which are evolutionarily conserved regulators of body patterning and organogenesis. The HOXD cluster, located on chromosome 2 in humans, encodes proteins that govern the development of limbs and the axial skeleton. HOXD8 specifically binds to TAAT-core DNA motifs and regulates downstream target genes involved in morphogenesis and cell differentiation.

Dysregulation of HOXD8 expression has been associated with altered developmental programs and has been studied in the context of various cancers and congenital abnormalities, making it a relevant target for both fundamental developmental biology research and translational studies.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human HOXD8 Protein (SKU: rp376613) is produced using recombinant expression systems to ensure consistent batch-to-batch quality. The protein is intended for research use only and is supplied with documentation to support experimental reproducibility.

Researchers should consult the product datasheet for confirmed details on purity grade, expression host, formulation buffer, molecular weight, and endotoxin levels, as these specifications are critical for downstream assay design. If specific lot information is required, contact Aladdin Scientific technical support.

Recommended Applications and Handling

Recombinant Human HOXD8 Protein is suitable for a range of in vitro research applications, including electrophoretic mobility shift assays (EMSA) to study DNA binding, co-immunoprecipitation or pull-down assays to investigate protein-protein interactions, and use as a positive control in Western blot or ELISA workflows validating HOXD8 antibodies.

When handling the protein, researchers should avoid repeated freeze-thaw cycles to preserve activity. It is recommended to aliquot the protein upon receipt and store aliquots at the temperature specified on the datasheet (typically -20°C or -80°C). Centrifuge the vial briefly before opening to collect the contents. Working dilutions should be prepared fresh in a compatible buffer immediately before use.

Storage, Stability, and Shipping

Recombinant proteins require careful attention to cold-chain logistics and storage conditions to maintain biological activity. Recombinant Human HOXD8 Protein is shipped under conditions designed to preserve protein integrity during transit. Upon receipt, immediately transfer the protein to the recommended storage temperature.

For long-term storage stability, refer to the product-specific datasheet. General best practices for recombinant proteins include storage at -80°C for extended periods and avoidance of storage in frost-free freezers that undergo temperature cycling. Adding a carrier protein such as BSA to diluted aliquots can help prevent adsorption losses at low concentrations.

Please note: specific stability data and shelf-life information for rp376613 should be confirmed from the official product datasheet or by contacting Aladdin Scientific customer support.

Ordering and Support

Recombinant Human HOXD8 Protein is available from Aladdin Scientific under SKU rp376613. To place an order, visit the Aladdin Scientific website, search by SKU or product name, and add the desired quantity to your cart. For bulk orders, custom formulations, or institutional procurement inquiries, contact Aladdin Scientific sales support directly.

Technical questions regarding experimental design, cross-reactivity, or application-specific recommendations for this protein can be directed to Aladdin Scientific's technical support team. Certificate of Analysis (CoA) and Safety Data Sheet (SDS) documents are available upon request or via the product page on the website.