Biological Background of HPDL

HPDL (4-hydroxyphenylpyruvate dioxygenase-like protein) is a nuclear-encoded gene product that localizes to the mitochondria. It belongs to the broader family of iron- and 2-oxoglutarate-dependent dioxygenases, sharing structural homology with 4-hydroxyphenylpyruvate dioxygenase (HPD), an enzyme involved in tyrosine catabolism and coenzyme Q biosynthesis pathways.

Emerging research has identified HPDL as a mitochondria-targeted protein whose precise enzymatic substrate and catalytic mechanism remain subjects of active investigation. Loss-of-function variants in the HPDL gene have been associated with a spectrum of early-onset, autosomal recessive neurological conditions, including spastic paraplegia and hypomyelinating leukodystrophy in pediatric patients. These clinical observations suggest that HPDL plays a non-redundant and essential role in maintaining neuronal and mitochondrial integrity.

The recombinant human HPDL protein provides researchers with a purified, well-defined tool to investigate the biochemical properties, substrate specificity, and interaction partners of this protein under controlled in vitro conditions.

Protein Properties and Quality Considerations

Recombinant Human HPDL Protein (rp367770) is produced using recombinant expression systems to ensure batch-to-batch consistency and high purity suitable for demanding biochemical assays. When evaluating a recombinant protein for research use, the following quality attributes should be considered:

• Purity: High-purity preparations (typically ≥90% by SDS-PAGE) minimize the risk of confounding results from contaminating proteins. Researchers should request a certificate of analysis (CoA) to verify lot-specific purity data.
• Biological Activity: Confirmation of activity through functional assays, where available, ensures that the recombinant protein retains native-like enzymatic or binding properties.
• Formulation and Stability: The buffer composition, presence of stabilizing agents (e.g., glycerol, BSA), and recommended storage temperature (typically -80°C for long-term storage) directly affect protein stability and experimental reproducibility.
• Endotoxin Levels: For cell-based assays and in vivo applications, low endotoxin content is critical to prevent non-specific inflammatory responses.

Researchers are advised to consult the product datasheet and CoA for rp367770 for lot-specific specifications. Contact Aladdin Scientific technical support for additional characterization data if required.

Recommended Handling and Storage Protocol

Proper handling and storage of Recombinant Human HPDL Protein is essential to preserve its integrity and activity. The following general guidelines apply to recombinant proteins of this class:

1. Upon Receipt: Inspect the shipment immediately. Recombinant proteins are typically shipped on dry ice. If the product has not remained frozen during transit, contact Aladdin Scientific customer service before use.
2. Reconstitution: If supplied lyophilized, reconstitute the protein according to the datasheet instructions using the recommended buffer. Allow the vial to equilibrate at room temperature for a few minutes, then gently pipette to dissolve. Avoid vortexing, which can denature the protein.
3. Aliquoting: To minimize freeze-thaw cycles, prepare single-use aliquots immediately after reconstitution. Repeated freeze-thaw cycles (generally more than 2–3) can significantly reduce protein activity and integrity.
4. Storage: Store aliquots at -80°C for long-term storage. Working aliquots may be kept at -20°C for short-term use, depending on formulation. Refer to the product datasheet for specific storage recommendations.
5. Working Concentrations: Optimize protein concentration empirically for each assay format. Pilot experiments using a concentration range are recommended before committing to large-scale assays.

Note: Always follow the specific instructions provided in the product datasheet for rp367770, as formulation-specific conditions may differ from these general guidelines.

Research Applications

Recombinant Human HPDL Protein (rp367770) is a versatile research reagent applicable across multiple experimental contexts:

• Enzyme Characterization Studies: Investigate the substrate specificity, cofactor requirements (e.g., iron, 2-oxoglutarate), and kinetic parameters of HPDL in vitro. Such studies are essential for defining the precise biochemical role of this enzyme.
• Protein-Protein Interaction Studies: Use purified HPDL protein in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments to identify and validate interaction partners within the mitochondrial proteome.
• Antibody Validation: Recombinant HPDL protein serves as a positive control antigen for validating anti-HPDL antibodies in Western blotting, ELISA, and immunoprecipitation applications.
• Structural Biology: High-purity recombinant protein preparations are suitable for crystallography, cryo-electron microscopy (cryo-EM), and other structural characterization approaches aimed at elucidating the three-dimensional structure of HPDL.
• Disease Mechanism Research: Given the association of HPDL loss-of-function with pediatric neurological disorders, recombinant HPDL protein can be used to study pathogenic mechanisms, screen for small-molecule modulators, or develop diagnostic tools.
• Coenzyme Q Biosynthesis Research: Given HPDL's homology to HPD and its mitochondrial localization, it may be a valuable tool in studying aspects of the CoQ biosynthesis pathway and related metabolic processes.

Ordering and Technical Support

Recombinant Human HPDL Protein is available from Aladdin Scientific under SKU rp367770. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative.

For technical inquiries regarding experimental design, recommended assay conditions, or interpretation of results with this product, please reach out to the Aladdin Scientific technical support team. Our scientists are available to assist with protocol optimization and troubleshooting.

Custom quantities, bulk orders, and special formulations may be available upon request. Please contact Aladdin Scientific customer service for further details on custom production options.

Reference internal product identifier: absea#PP-18234 (rp367770)