Recombinant Human HPS5 Protein: FAQs & Technical Guide
Recombinant Human HPS5 Protein: FAQs & Technical Guide
Recombinant Human HPS5 Protein is a key research reagent enabling scientists to investigate the molecular mechanisms of lysosome-related organelle biogenesis and Hermansky-Pudlak syndrome (HPS).
- Product Name
- Recombinant Human HPS5 Protein
- SKU
- rp375104
- Gene / Protein
- HPS5 (Hermansky-Pudlak Syndrome 5 Protein)
- Biological Role
- Subunit of the BLOC-2 complex; involved in lysosome-related organelle biogenesis
- Disease Relevance
- Hermansky-Pudlak Syndrome (HPS), a rare autosomal recessive disorder
- Supplier
- Aladdin Scientific
What Is HPS5 and Why Is It Important?
HPS5 (Hermansky-Pudlak Syndrome Protein 5) is a subunit of the biogenesis of lysosome-related organelles complex 2 (BLOC-2). BLOC-2 plays a critical role in the trafficking and biogenesis of lysosome-related organelles (LROs), including melanosomes in melanocytes and dense granules in platelets.
Loss-of-function mutations in the HPS5 gene cause a subtype of Hermansky-Pudlak Syndrome, a rare autosomal recessive disorder characterized by oculocutaneous albinism, bleeding diathesis due to platelet dysfunction, and in some cases pulmonary fibrosis. Research into HPS5 protein interactions and BLOC-2 complex assembly is therefore of significant clinical and cell biological interest.
Recombinant Human HPS5 Protein from Aladdin Scientific (SKU: rp375104) provides researchers with a high-quality, ready-to-use tool for mechanistic studies, protein-interaction assays, and antibody validation.
Protein Specifications and Quality
When selecting a recombinant protein for critical experiments, understanding the specifications is essential. Below are key quality attributes researchers should verify before use:
- Expression System: Please confirm the expression host (e.g., E. coli, baculovirus/insect cells, or mammalian cells) with the product datasheet or Aladdin Scientific technical support, as this affects post-translational modifications and functional activity.
- Purity: Recombinant proteins from Aladdin Scientific are typically manufactured to high purity standards; refer to the Certificate of Analysis (CoA) for lot-specific purity data determined by SDS-PAGE or HPLC.
- Tag Information: The presence and position of affinity tags (e.g., His-tag, GST-tag) should be confirmed via the product datasheet, as tags may influence binding assays or require removal for certain applications.
- Formulation and Concentration: Refer to the product datasheet for buffer composition, protein concentration, and any carrier proteins such as BSA included in the formulation.
Note: Specific numerical values for purity, molecular weight, and concentration are lot-dependent. Always consult the accompanying CoA for accurate lot-specific data.
Storage, Handling, and Reconstitution
Proper storage and handling are critical to maintaining the activity and integrity of Recombinant Human HPS5 Protein. The following general best practices apply; always defer to the product datasheet for product-specific instructions:
- Storage Temperature: Recombinant proteins are generally stored at −20 °C or −80 °C for long-term stability. Avoid repeated freeze-thaw cycles, which can cause protein aggregation and activity loss.
- Aliquoting: Upon receipt, it is recommended to prepare single-use aliquots before freezing to minimize freeze-thaw degradation.
- Reconstitution: If supplied in lyophilized form, reconstitute with the volume and buffer specified in the datasheet. Allow the protein to dissolve gently; do not vortex vigorously. Centrifuge briefly to collect the solution.
- Working Stability: Once reconstituted or thawed, keep the protein on ice and use within the timeframe recommended in the datasheet. Some proteins tolerate short-term storage at 4 °C for up to 1 week with the addition of a carrier protein.
Contact Aladdin Scientific technical support at any time if you encounter handling issues or require application-specific guidance.
Research Applications of HPS5 Recombinant Protein
Recombinant Human HPS5 Protein is suitable for a variety of in vitro research applications. Typical uses include:
- Protein–Protein Interaction Studies: HPS5 is a constituent of the BLOC-2 complex, making it valuable for pull-down assays, co-immunoprecipitation controls, and surface plasmon resonance (SPR) experiments to characterize interactions with HPS6, HPS3, and other BLOC-2 partners.
- Antibody Validation: Recombinant HPS5 protein serves as a positive control antigen for ELISA, Western blot, and dot-blot assays when validating anti-HPS5 antibodies.
- Biochemical Assays: The protein can be used in functional assays designed to study BLOC-2 complex assembly and its role in endosomal trafficking pathways.
- Cell-Free Systems: In vitro reconstitution experiments using purified BLOC-2 subunits, including HPS5, allow mechanistic dissection of organelle biogenesis without cellular complexity.
- Drug Discovery: HPS5 and the BLOC-2 complex represent potential targets for understanding and modelling HPS pathology, supporting early-stage screening workflows.
Always verify that the expression system and tag configuration of the recombinant protein are compatible with your specific assay format.
Ordering and Technical Support
Recombinant Human HPS5 Protein is available from Aladdin Scientific under SKU rp375104. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.
Technical Support: Aladdin Scientific's technical support team can assist with questions regarding protein activity, assay compatibility, custom formulations, and bulk ordering. Reach out via the contact form on the Aladdin Scientific website.
Documentation: Certificate of Analysis (CoA), Safety Data Sheet (SDS), and product datasheet are available upon request or via the product page. Lot-specific CoAs contain critical quality control data including purity, endotoxin level, and biological activity where applicable.
Returns and Replacements: If you receive a product that does not meet the stated specifications, please contact Aladdin Scientific customer service within the designated return window as outlined in the Returns and Refunds policy.
Frequently asked questions
What is the function of HPS5 protein in human cells?
HPS5 is a subunit of the BLOC-2 (Biogenesis of Lysosome-Related Organelles Complex 2) complex, which is required for the proper biogenesis and trafficking of lysosome-related organelles such as melanosomes and platelet dense granules. Mutations in HPS5 cause a subtype of Hermansky-Pudlak Syndrome, resulting in albinism and platelet dysfunction.
What expression system is used to produce Recombinant Human HPS5 Protein (rp375104)?
The specific expression system used for rp375104 is detailed in the product datasheet and Certificate of Analysis. Please download the datasheet from the Aladdin Scientific product page or contact technical support for this information, as the expression host determines the protein's post-translational modification profile and is critical for selecting the appropriate application.
How should Recombinant Human HPS5 Protein be stored to maintain its activity?
As a general guideline, recombinant proteins should be stored at −20 °C or −80 °C in single-use aliquots to avoid repeated freeze-thaw cycles that can compromise activity and promote aggregation. Always consult the product-specific datasheet for the recommended storage conditions, buffer, and shelf life for rp375104.
What are the recommended applications for Recombinant Human HPS5 Protein?
Recombinant Human HPS5 Protein is commonly used for protein–protein interaction studies (e.g., pull-down, SPR), antibody validation (Western blot, ELISA positive control), in vitro BLOC-2 complex reconstitution, and as a tool in early drug discovery workflows related to Hermansky-Pudlak Syndrome. Confirm compatibility of the specific formulation and tag with your assay before use.
Is a Certificate of Analysis (CoA) available for rp375104?
Yes. A lot-specific Certificate of Analysis is available for Recombinant Human HPS5 Protein (rp375104). You can request it through the Aladdin Scientific product page or by contacting customer service with your lot number. The CoA includes key quality control data such as purity, concentration, and endotoxin level.
Can Recombinant Human HPS5 Protein be used for in vivo studies?
Recombinant Human HPS5 Protein (rp375104) is intended for in vitro research use only and is not approved for clinical, therapeutic, or in vivo diagnostic applications. Researchers planning in vivo studies should consult the datasheet for endotoxin specifications and contact Aladdin Scientific technical support to discuss suitability.
References
- Di Pietro SM, Falcon-Perez JM, Tenza D, Setty SR, Bhatt AM, Bhatt MN, et al. BLOC-1 interacts with BLOC-2 and the AP-3 complex to facilitate protein trafficking on endosomes. Mol Biol Cell. 2006.
- Huizing M, Helip-Wooley A, Westbroek W, Gunay-Aygun M, Gahl WA. Disorders of lysosome-related organelle biogenesis: clinical and molecular genetics. Annu Rev Genomics Hum Genet. 2008;9:359-386.
- Raposo G, Marks MS. Melanosomes--dark organelles enlighten endosomal membrane transport. Nat Rev Mol Cell Biol. 2007;8(10):786-797.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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