Background: HSP90 Family and HSP90AB4P

Heat shock proteins of the HSP90 family are highly conserved molecular chaperones that play central roles in the folding, stabilization, and degradation of a wide range of client proteins involved in signal transduction, cell cycle control, and stress response. The HSP90 family in humans includes canonical members such as HSP90AA1 (HSP90α) and HSP90AB1 (HSP90β), which are expressed ubiquitously and participate in numerous cellular processes.

HSP90AB4P is annotated in the human genome as a pseudogene belonging to the HSP90 alpha family class B lineage. Pseudogene-derived sequences and their protein products have attracted growing research interest, as some pseudogene loci are transcribed and may give rise to protein products or regulatory RNAs with functional relevance. Recombinant Human HSP90AB4P Protein is produced to enable researchers to investigate the biochemical properties, potential interactions, and functional significance of the HSP90AB4P locus-derived sequence.

Note: Because HSP90AB4P is classified as a pseudogene, its precise biological function remains an active area of investigation. Researchers should consult the current literature and validate experimental findings with appropriate controls.

Product Specifications and Quality Considerations

Recombinant Human HSP90AB4P Protein (SKU: rp373927) is manufactured as a recombinant protein for research applications. When evaluating this reagent, researchers and procurement personnel should consider the following key specification parameters:

• Purity: Recombinant proteins intended for biochemical assays are typically assessed by SDS-PAGE and/or HPLC. Please refer to the Certificate of Analysis (CoA) supplied with each lot for confirmed purity data.
• Expression System: The expression host (e.g., E. coli, insect cells, mammalian cells, or yeast) determines post-translational modification status and solubility. Consult the product datasheet for confirmed expression system details.
• Formulation and Storage: Recombinant proteins are commonly supplied lyophilized or in stabilizing buffer. Recommended storage temperature, reconstitution buffer, and working concentration should be followed precisely as described in the product datasheet to preserve activity.
• Endotoxin Level: For cell-based assays, endotoxin content should be verified from the CoA to avoid non-specific cellular responses.

All specific numerical values for purity, concentration, and endotoxin levels are lot-specific; always refer to the CoA for the supplied lot.

Handling, Reconstitution, and Storage Guidelines

Proper handling of recombinant proteins is essential to maintain their integrity and experimental reproducibility. The following general best practices apply to Recombinant Human HSP90AB4P Protein and should be supplemented with the specific instructions provided in the product datasheet:

1. Reconstitution: If supplied lyophilized, allow the vial to equilibrate to room temperature before opening. Reconstitute in the recommended volume of sterile, ultrapure water or the buffer specified in the datasheet. Gently mix by pipetting; avoid vortexing to prevent denaturation.
2. Aliquoting: To minimize freeze-thaw cycles, prepare single-use aliquots immediately after reconstitution. Multiple freeze-thaw cycles can significantly reduce protein activity and structural integrity.
3. Storage Temperature: Store at the temperature specified on the datasheet (typically −20°C or −80°C for long-term storage). Short-term storage at 4°C may be acceptable for working solutions per datasheet instructions.
4. Carrier Protein: Addition of a carrier protein such as bovine serum albumin (BSA) at 0.1–0.5% may enhance stability during storage of dilute solutions, unless the intended assay is incompatible.
5. Avoid Contamination: Use aseptic technique. Protein degradation by proteases can be minimized by keeping solutions on ice during handling.

Potential Research Applications

Recombinant Human HSP90AB4P Protein may be employed in a variety of research contexts, particularly those focused on HSP90 biology, pseudogene function, and protein–protein interactions. Representative applications include:

• Biochemical Assays: Use as a substrate or binding partner in in vitro pull-down, co-immunoprecipitation (co-IP), or surface plasmon resonance (SPR) experiments to characterize molecular interactions.
• Antibody Validation: Serve as a positive control antigen for validating antibodies raised against HSP90AB4P or cross-reactive HSP90 epitopes in Western blot, ELISA, or immunofluorescence experiments.
• Structural Studies: Support structural biology investigations such as circular dichroism (CD) spectroscopy or cryo-electron microscopy sample preparation when studying HSP90 family protein architecture.
• Functional Studies: Investigate the potential chaperone activity or lack thereof of HSP90AB4P-derived protein in refolding assays or ATPase activity assays, benchmarked against canonical HSP90 family members.
• Cell-Based Research: Introduce recombinant protein into cell-free systems or cell culture models to assess biological activity and downstream signaling effects.

Important: Experimental design should include appropriate negative and positive controls. Given the pseudogene status of HSP90AB4P, careful interpretation of functional data is advised.

Ordering, Quality Assurance, and Support

Recombinant Human HSP90AB4P Protein is available from Aladdin Scientific under SKU rp373927. To ensure a smooth procurement experience, consider the following:

• Ordering: Place orders through the Aladdin Scientific website or contact your regional sales representative. Provide the SKU (rp373927) to confirm you are ordering the correct product.
• Certificate of Analysis (CoA): A lot-specific CoA is available upon request or download from the product page. Verify purity, concentration, and endotoxin data before initiating experiments.
• Technical Support: Aladdin Scientific's technical support team can assist with questions regarding reconstitution, storage, and application optimization. Contact details are available on the company website.
• Returns and Refunds: For information on returns or damaged shipments, refer to Aladdin Scientific's return and refund policy on the website.
• Shipping: Recombinant proteins are typically shipped on dry ice or with cold packs. Confirm shipping conditions at the time of ordering to ensure cold chain integrity upon receipt.