What Is HUWE1 and Why Is It Important in Research?

HUWE1 (also known as HECTH9, ARF-BP1, LASU1, MULE, and UreB1) is a large HECT-domain E3 ubiquitin ligase encoded by the HUWE1 gene on the X chromosome. As an E3 ligase, HUWE1 catalyzes the final step in the ubiquitin transfer cascade, mediating the attachment of ubiquitin to specific substrate proteins and thereby marking them for proteasomal degradation or altering their activity and localization.

HUWE1 has been shown to regulate a diverse range of substrates with critical roles in cell cycle progression, apoptosis, DNA damage response, and stem cell maintenance. Key reported substrates include tumor suppressors, pro-apoptotic factors, and oncoproteins, making HUWE1 a subject of considerable interest in cancer biology, neuroscience, and developmental biology research. Its dysregulation has been associated with multiple cancers and neurodevelopmental disorders.

Product Specifications and Quality

Recombinant Human HUWE1 Protein (SKU: rp370980) is produced for research use and is intended to provide a reliable source of active or near-native HUWE1 for in vitro biochemical studies. The product is referenced under supplier code absea#PP-5181.

Researchers should consult the product datasheet for confirmed specifications including: expression host system (commonly insect or mammalian cell systems for large HECT-domain ligases), purity grade, molecular weight, formulation buffer, and any activity data. Purity is typically assessed by SDS-PAGE, and activity may be confirmed by ubiquitination assay. Always verify current lot-specific data from the certificate of analysis (CoA) available through Aladdin Scientific.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human HUWE1 Protein are essential to preserve protein integrity and biological activity. As a general guideline for recombinant proteins of this class:

• Store at −80°C upon receipt for long-term storage.
• Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity. Prepare single-use aliquots before freezing.
• Keep on ice during experimental setup and minimize time at room temperature.
• Centrifuge briefly before opening to collect any condensate.

Refer to the product datasheet and CoA for lot-specific recommendations on storage buffer composition, recommended working concentration, and shelf life. Contact Aladdin Scientific technical support if stability concerns arise.

Recommended Research Applications

Recombinant Human HUWE1 Protein is suitable for a range of in vitro research applications, including but not limited to:

• In vitro ubiquitination assays: Investigate HUWE1-mediated ubiquitin transfer to candidate substrates in reconstituted E1-E2-E3 cascade systems.
• Enzyme kinetics studies: Characterize HUWE1 catalytic activity, substrate specificity, and the effects of small-molecule modulators.
• Protein–protein interaction studies: Use in pull-down assays, co-immunoprecipitation setups, or surface plasmon resonance to identify HUWE1 binding partners.
• Drug discovery and screening: Deploy as a target protein in inhibitor screening assays relevant to oncology and neurological disease research.
• Structural and biochemical studies: Support structural biology efforts aimed at understanding the HECT domain mechanism.

This product is intended for research use only and is not suitable for diagnostic or therapeutic applications.

Ordering and Technical Support

Recombinant Human HUWE1 Protein is available from Aladdin Scientific under SKU rp370980. To place an order, visit the Aladdin Scientific website, search by SKU or product name, and proceed through the standard ordering workflow. Bulk quantities and custom formulations may be available upon request.

For technical questions regarding experimental design, buffer compatibility, or activity validation, contact the Aladdin Scientific technical support team through the website's Help & Customer Service portal. For questions about pricing, promotions, shipping, and returns, refer to the relevant policy pages or contact customer service directly.