What Is IGLL1 and Why Is It Important in Research?

IGLL1 (Immunoglobulin Lambda-Like Polypeptide 1), also designated CD179b or IGL1, encodes a surrogate light chain protein that pairs with the mu heavy chain and the VpreB protein to form the pre-B cell receptor (pre-BCR). This receptor complex is indispensable for signaling checkpoints during early B lymphocyte development, facilitating the transition from the pro-B cell stage to the pre-B cell stage.

Mutations or deficiencies in IGLL1 have been associated with autosomal recessive agammaglobulinemia, a primary immunodeficiency in which B-cell maturation is severely impaired. As a result, IGLL1 is a biologically significant target in immunology, hematology, and primary immunodeficiency disease research. Recombinant Human IGLL1 Protein (rp369662) enables researchers to study these pathways in a controlled in vitro environment.

Product Specifications and Quality Attributes

Recombinant Human IGLL1 Protein (SKU: rp369662) is produced using recombinant expression technology to deliver a high-quality, research-grade reagent. Key quality attributes researchers should be aware of include:

• Purity: Recombinant proteins from Aladdin Scientific are manufactured and tested to meet research-grade purity standards. Specific purity data (e.g., SDS-PAGE or HPLC results) should be confirmed on the product datasheet.
• Formulation: Typically supplied as a lyophilized or liquid preparation; refer to the specific lot Certificate of Analysis (CoA) for exact formulation details.
• Endotoxin Level: Endotoxin testing is performed to ensure suitability for cell-based assays; confirm specific endotoxin limits on the product CoA.
• Source/Host: Expression host information (e.g., E. coli, HEK293, or baculovirus system) should be verified on the product datasheet for rp369662, as this affects glycosylation status and downstream application compatibility.

Reconstitution, Handling, and Storage Guidelines

Proper handling of Recombinant Human IGLL1 Protein is essential to preserve biological activity and ensure reproducible results. Follow these general best practices:

• Reconstitution: For lyophilized preparations, reconstitute in sterile deionized water or the buffer specified in the product datasheet to the recommended concentration. Allow the vial to equilibrate to room temperature before opening, and gently swirl to dissolve — do not vortex.
• Working Aliquots: Prepare single-use aliquots immediately after reconstitution to minimize degradation from repeated freeze-thaw cycles.
• Short-term Storage: Reconstituted protein can typically be stored at 4°C for up to one week, depending on formulation. Confirm with the product-specific CoA.
• Long-term Storage: Store lyophilized protein at -20°C to -80°C. For reconstituted protein intended for long-term storage, the addition of a carrier protein (e.g., 0.1% BSA) may stabilize activity.
• Avoid Repeated Freeze-Thaw: Each freeze-thaw cycle can reduce protein activity. Aliquot before freezing to avoid this.

Recommended Applications for IGLL1 Protein in Research

Recombinant Human IGLL1 Protein is suitable for a range of in vitro research applications. Common uses include:

• ELISA (Enzyme-Linked Immunosorbent Assay): Used as a standard or coating antigen to develop or validate IGLL1-specific detection assays.
• Western Blot: Can serve as a positive control for antibody validation experiments targeting IGLL1.
• Functional/Bioassays: Used to study pre-BCR signaling, B-cell differentiation checkpoints, or receptor-ligand interactions in vitro.
• Cell-Based Assays: Applied to investigate IGLL1-mediated cellular responses in B-cell or immunology models.
• Protein-Protein Interaction Studies: Useful for characterizing the interaction between IGLL1, VpreB, and the mu heavy chain in surrogate light chain complex assembly.

Always validate the protein's activity for your specific assay conditions using appropriate positive and negative controls.

Ordering, Shipping, and Support

Recombinant Human IGLL1 Protein (SKU: rp369662) is available through Aladdin Scientific. To order or obtain additional information:

• Ordering: Place orders online via the Aladdin Scientific website or contact your regional sales representative. Reference SKU rp369662 when ordering.
• Shipping: Cold-chain shipping conditions are applied to maintain protein integrity during transit. Please ensure appropriate cold-storage infrastructure is available upon receipt.
• Documentation: A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available for each product lot. Contact customer service or download directly from the product page.
• Technical Support: For application-specific guidance, reconstitution advice, or troubleshooting, contact Aladdin Scientific's technical support team.
• Returns and Refunds: Please refer to Aladdin Scientific's returns and refund policy for reagent products. Contact customer service within the specified timeframe for any quality-related concerns.