What Is IL1RAPL1 and Why Is It Important in Research?

IL1RAPL1 (Interleukin-1 Receptor Accessory Protein-Like 1) is a member of the interleukin-1 receptor superfamily and is encoded by a gene located on the X chromosome. It plays a critical role in the formation and stabilization of excitatory synapses in the central nervous system. IL1RAPL1 interacts with presynaptic partners such as PTP-delta (PTPRD), facilitating trans-synaptic signaling that promotes dendritic spine development and synapse maturation.

Mutations and deletions in the IL1RAPL1 gene have been associated with X-linked intellectual disability and autism spectrum disorder (ASD), making this protein a significant target for researchers studying the molecular basis of neurodevelopmental conditions. Recombinant Human IL1RAPL1 Protein enables in vitro investigation of these interactions without the need for primary neuronal culture or animal models at every experimental step.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human IL1RAPL1 Protein (SKU: rp366197) is produced using a recombinant expression system designed to yield a functional extracellular domain fragment suitable for biochemical and cell-based assays. Key quality attributes typically evaluated for recombinant proteins of this class include:

• Purity: Assessed by SDS-PAGE and/or HPLC; target purity is generally >90% (verify exact specification on the product datasheet).
• Endotoxin Level: Tested by LAL assay to ensure suitability for cell-based applications (specific threshold on datasheet).
• Biological Activity: Confirmed via binding assays or functional cell-based assays where applicable.
• Formulation: Typically supplied lyophilized or in a carrier-protein-containing buffer; refer to the current datasheet for exact formulation details.

All specification values should be verified against the current Certificate of Analysis (CoA) supplied with each lot.

Reconstitution, Handling, and Storage Guidelines

Proper handling of recombinant proteins is essential to maintain activity and avoid degradation. The following general guidelines apply to Recombinant Human IL1RAPL1 Protein; always confirm with the product-specific datasheet before use:

1. Reconstitution: If supplied lyophilized, reconstitute in sterile, deionized water or the buffer specified in the datasheet to achieve the recommended stock concentration. Allow the vial to equilibrate to room temperature before opening.
2. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which can reduce protein activity.
3. Short-term storage: Store at 4°C for up to 1 week if the protein will be used shortly after reconstitution.
4. Long-term storage: Store at -20°C or -80°C. Adding a carrier protein such as 0.1% BSA may improve stability during long-term storage (confirm with datasheet).
5. Avoid: Repeated freeze-thaw cycles, prolonged exposure to room temperature, and detergent contamination.

Typical Research Applications

Recombinant Human IL1RAPL1 Protein is suitable for a range of in vitro and biochemical research applications, including:

• Protein–Protein Interaction Studies: Investigating the binding of IL1RAPL1 extracellular domain to synaptic partners such as PTPRD, using techniques including SPR, co-immunoprecipitation, and ELISA-based binding assays.
• Synaptogenesis Assays: Using recombinant IL1RAPL1 to induce or inhibit synapse formation in neuronal co-culture systems.
• Antibody Validation: Serving as a positive control antigen for Western blot, ELISA, and immunofluorescence assay validation.
• Drug Discovery and Screening: As a target protein in binding screens to identify small molecules or biologics that modulate IL1RAPL1 interactions relevant to ASD or intellectual disability.
• Structural Biology: Supporting crystallography or cryo-EM studies of the IL1RAPL1 extracellular domain in complex with binding partners.

Researchers are encouraged to consult the product datasheet and relevant literature to confirm suitability for specific experimental formats.

Ordering, Availability, and Support

Recombinant Human IL1RAPL1 Protein is available exclusively through Aladdin Scientific under SKU rp366197. To place an order or request a quote, visit the Aladdin Scientific website or contact our customer service team directly.

Technical Support: Our scientific support team can assist with questions regarding reconstitution protocols, recommended assay conditions, compatibility with downstream applications, and lot-specific quality data. Please have your SKU and lot number ready when contacting support.

Returns and Quality Concerns: If the product does not meet the specifications described in the Certificate of Analysis, please contact Aladdin Scientific within the timeframe specified in our returns policy. Proof of purchase and lot number will be required.

Bulk and Custom Inquiries: For large-quantity orders or custom formulations (e.g., specific buffer, concentration, or tag), please contact our sales team to discuss feasibility and pricing.