Background: What Is ILDR1?

ILDR1 (Immunoglobulin-Like Domain-Containing Receptor 1) is a single-pass transmembrane protein encoded by the ILDR1 gene in humans. It belongs to a small family of receptors characterized by an extracellular immunoglobulin-like (Ig-like) fold, a transmembrane domain, and a cytoplasmic tail. ILDR1 is predominantly expressed in epithelial tissues, including the cochlea of the inner ear and various visceral epithelia.

Loss-of-function mutations in ILDR1 have been causally linked to DFNB42, a form of autosomal recessive non-syndromic sensorineural hearing loss. Mechanistically, ILDR1 localizes to tricellular tight junctions—specialized junctions at the vertices where three epithelial cells meet—and is required for the proper assembly and barrier function of these structures. Its role in maintaining epithelial integrity makes it a target of interest in hearing biology, epithelial barrier research, and related disease models.

Protein Specifications & Quality

The Recombinant Human ILDR1 Protein (SKU: rp368367) is produced using established recombinant expression systems to ensure consistency and reproducibility across experiments. Researchers should consult the product datasheet for lot-specific details including:

• Expression system: Refer to current datasheet (e.g., mammalian, insect, or E. coli-based; confirm with Aladdin Technical Support)
• Purity: Typically ≥85% as determined by SDS-PAGE; exact purity confirmed per lot certificate of analysis
• Formulation: Supplied in a carrier-protein-containing buffer; exact formulation listed on the datasheet
• Molecular weight: Confirm theoretical and observed MW from the current datasheet, as glycosylation or tags may affect migration
• Endotoxin level: Verified per lot; critical for cell-based functional assays

All specifications should be verified against the official Certificate of Analysis (CoA) provided with each shipment. Contact Aladdin Scientific customer service for lot-specific CoA documentation.

Handling, Reconstitution & Storage Protocol

Proper handling of recombinant proteins is essential to preserve activity and ensure reproducible results. Follow the guidelines below for Recombinant Human ILDR1 Protein:

1. Receipt: Upon arrival, inspect the product and store immediately at the recommended temperature. Do not allow the protein to remain at room temperature for extended periods.
2. Reconstitution: If supplied lyophilized, reconstitute in the volume of sterile, ultrapure water or buffer specified on the datasheet. Allow the vial to equilibrate at room temperature for a few minutes before gentle mixing. Avoid vortexing; instead, pipette gently to dissolve.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which can degrade protein integrity and reduce biological activity.
4. Storage: Store at -20°C for short-term use (up to 1 month) or -80°C for long-term storage. Avoid repeated freeze-thaw cycles (maximum 2–3 cycles recommended as a general guideline; confirm with datasheet).
5. Working concentrations: Determine empirically for each application. Refer to the datasheet for suggested starting concentrations for ELISA, Western blot, and functional assays.

Note: Specific reconstitution volumes, buffer compositions, and storage durations must be confirmed from the product datasheet or CoA supplied with the lot.

Research Applications

Recombinant Human ILDR1 Protein is suited for a range of in vitro and biochemical research applications:

• ELISA: Use as a standard or capture/detection antigen in sandwich or competitive ELISA formats to measure ILDR1 levels in experimental systems.
• Western Blot (WB): Employ as a positive control to validate anti-ILDR1 antibody specificity and sensitivity.
• Antibody Development & Screening: Immunize animals or use in phage/yeast display screens to generate and validate ILDR1-specific antibodies for research or diagnostic purposes.
• Protein–Protein Interaction Studies: Investigate ILDR1 binding partners involved in tricellular tight-junction assembly using pull-down or co-immunoprecipitation approaches.
• Functional & Cell-Based Assays: Explore the role of ILDR1 in epithelial barrier formation and permeability in relevant cell models.
• Structural Studies: Serve as material for biophysical characterization, including circular dichroism or limited proteolysis experiments.

Researchers studying hereditary hearing loss (DFNB42), epithelial barrier dysfunction, or tight-junction biology will find this protein particularly relevant.

Ordering & Technical Support

Recombinant Human ILDR1 Protein is available from Aladdin Scientific under SKU rp368367. To place an order or request a quote, contact your regional Aladdin Scientific representative or use the online ordering portal.

Technical Support: For application-specific guidance, reconstitution assistance, or datasheet clarification, contact Aladdin Scientific Technical Support. Please have your SKU (rp368367) and lot number ready when contacting support.

Documentation available upon request:

• Certificate of Analysis (CoA)
• Safety Data Sheet (SDS)
• Product Datasheet

For returns, refunds, or shipping inquiries, refer to Aladdin Scientific's standard policies available on the company website.