What Is INCENP and Why Is It Important in Research?

INCENP (Inner Centromere Protein) is a core scaffolding component of the Chromosomal Passenger Complex (CPC), a multiprotein assembly that is essential for accurate chromosome segregation, spindle assembly checkpoint signaling, and cytokinesis during cell division. The CPC consists of four subunits: INCENP, Aurora B kinase, Survivin, and Borealin. INCENP serves dual roles: it acts as a structural scaffold that localizes the complex to the inner centromere and subsequently to the spindle midzone, and it directly activates Aurora B kinase through interaction via its C-terminal IN-box domain.

Because of these pivotal functions, INCENP is a subject of intense study in cancer biology, mitosis research, and drug target validation. Dysregulation of CPC components including INCENP has been linked to chromosomal instability, a hallmark of many cancers. Recombinant Human INCENP Protein enables researchers to study these mechanisms in defined biochemical systems.

What Are the Recommended Applications for This Protein?

Recombinant Human INCENP Protein (rp364998) is suitable for a range of in vitro research applications, including but not limited to:

• Biochemical assays: Used as a substrate or binding partner in kinase activity assays, particularly those investigating Aurora B activation.
• Protein–protein interaction studies: Suitable for co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) experiments to map CPC subunit interactions.
• Structural biology: Can serve as a component for cryo-EM or X-ray crystallography studies of CPC architecture.
• Antibody validation: Useful as a positive control antigen for validating anti-INCENP antibodies in Western blot and ELISA formats.
• Drug discovery: Applicable in inhibitor screening assays targeting CPC-mediated Aurora B activation.

Researchers should validate the protein for each specific application under their own experimental conditions. Please contact Aladdin Scientific technical support for application-specific guidance.

Storage, Handling, and Stability Guidelines

Proper storage and handling of Recombinant Human INCENP Protein are critical to maintaining its activity and integrity. General best-practice guidelines for recombinant proteins of this class include:

• Storage temperature: Store at −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze–thaw cycling.
• Working conditions: Keep on ice during experimental use. Avoid prolonged exposure to room temperature.
• Reconstitution: If supplied lyophilized, reconstitute in the recommended buffer as specified on the product datasheet. Centrifuge briefly before use to collect any aggregates.
• Expiry: Refer to the Certificate of Analysis (CoA) for lot-specific expiry and stability data.

Always consult the product-specific datasheet provided with your order (absea#PP-399) for authoritative storage and handling instructions.

Quality, Purity, and Specifications

Aladdin Scientific's Recombinant Human INCENP Protein (rp364998) is produced to research-reagent standards. Key quality attributes typically verified for recombinant proteins in this category include:

• Purity: Assessed by SDS-PAGE and/or HPLC. Specific purity values are provided on the lot-specific Certificate of Analysis (CoA).
• Identity: Confirmed by mass spectrometry or N-terminal sequencing.
• Endotoxin levels: Tested by LAL assay; endotoxin specification is stated on the CoA.
• Biological activity: Functional data, where available, are reported on the product datasheet.

For precise molecular weight, expression system, tag information, buffer composition, and concentration, please refer to the official product page for rp364998 on the Aladdin Scientific website or contact our technical support team. Lot-to-lot consistency is ensured through our quality management processes.