Recombinant Human ING3 Protein: FAQs & Key Facts
Recombinant Human ING3 Protein: FAQs & Key Facts
Recombinant Human ING3 Protein is a research-grade recombinant protein derived from the human ING3 gene, belonging to the inhibitor of growth (ING) family of tumor suppressors involved in chromatin remodeling, cell cycle regulation, and apoptosis.
- Product Name
- Recombinant Human ING3 Protein
- SKU
- rp376578
- Protein Family
- ING (Inhibitor of Growth) family, tumor suppressor proteins
- Key Biological Functions
- Chromatin remodeling, cell cycle regulation, apoptosis induction, and DNA damage response
- Research Applications
- Biochemical assays, protein interaction studies, chromatin biology, and cancer research
- Supplier Reference
- absea#PP-10126
What Is Recombinant Human ING3 Protein?
ING3 (Inhibitor of Growth Family Member 3) is a member of the highly conserved ING tumor suppressor protein family. ING family proteins share a plant homeodomain (PHD) finger motif that enables them to bind histone H3 trimethylated at lysine 4 (H3K4me3), a chromatin mark associated with active transcription. Through this interaction, ING3 participates in the regulation of gene expression, DNA repair, and cell cycle progression.
Recombinant Human ING3 Protein (SKU: rp376578) is produced recombinantly to provide researchers with a well-defined, research-grade tool for studying ING3 biology in vitro. It is suitable for laboratories investigating chromatin remodeling complexes, tumor suppression mechanisms, and related signaling pathways.
Biological Role and Research Significance of ING3
ING3 functions as a stoichiometric component of the NuA4 histone acetyltransferase complex (also known as the TIP60 complex in mammals). This complex acetylates histone H4 and plays a pivotal role in regulating DNA damage checkpoints and apoptotic responses. Loss or downregulation of ING3 expression has been reported in several human cancers, supporting its role as a tumor suppressor.
Key research areas where ING3 is relevant include:
- Chromatin remodeling: ING3 bridges histone H3K4me3 recognition with HAT complex activity to modulate chromatin accessibility.
- Apoptosis and DNA damage response: ING3 promotes UV-induced apoptosis and participates in the cellular response to genotoxic stress.
- Cancer biology: Reduced ING3 levels have been associated with various malignancies, making it a subject of interest in oncology research.
Storage, Handling, and Quality Considerations
Proper storage and handling of Recombinant Human ING3 Protein (rp376578) are essential to maintain protein integrity and biological activity. As with most recombinant proteins, the following general best practices apply:
- Storage temperature: Store at -20°C or -80°C upon receipt; avoid repeated freeze-thaw cycles by preparing single-use aliquots.
- Reconstitution: Follow the specific reconstitution instructions provided in the product datasheet. Use the recommended buffer to achieve optimal solubility and activity.
- Quality assessment: Lot-specific quality data, including purity analysis (typically assessed by SDS-PAGE) and biological activity information, are available upon request or on the product datasheet.
Note: Specific storage conditions, purity grade, and activity data for rp376578 should be confirmed against the current product datasheet. Contact Aladdin Scientific technical support for the most up-to-date certificate of analysis.
Recommended Applications for Recombinant Human ING3 Protein
Recombinant Human ING3 Protein is designed to support a range of in vitro research applications. Typical uses include:
- Protein-protein interaction studies: Investigate ING3 interactions with components of the NuA4/TIP60 complex or other chromatin-associated factors using co-immunoprecipitation, pull-down assays, or surface plasmon resonance.
- Biochemical and enzymatic assays: Use as a substrate or binding partner in assays designed to characterize HAT complex activity or histone-binding specificity.
- Antibody validation: Serve as a positive control antigen for validating anti-ING3 antibodies in Western blot or ELISA applications.
- Structural and biophysical studies: Support crystallography, cryo-EM preparation, or biophysical characterization of ING3 and its complexes.
Researchers should consult the product datasheet for validated application-specific conditions and recommended concentrations.
Ordering and Technical Support
Recombinant Human ING3 Protein is available from Aladdin Scientific under SKU rp376578. When placing an order, please reference this SKU to ensure accurate fulfillment. The product is also referenced under supplier code absea#PP-10126.
For technical questions regarding experimental design, compatibility, or troubleshooting, please contact the Aladdin Scientific technical support team. For procurement inquiries including bulk pricing, certificate of analysis requests, or custom arrangements, contact the sales team directly through the Aladdin Scientific website.
Frequently asked questions
What is the biological function of ING3 and why is it important in cancer research?
ING3 is a tumor suppressor protein and a core subunit of the NuA4/TIP60 histone acetyltransferase complex. It recognizes the H3K4me3 chromatin mark via its PHD finger domain, linking active histone marks to chromatin remodeling and gene regulation. ING3 downregulation has been reported in several cancers, making it relevant for studies into tumor suppression, apoptosis, and DNA damage response.
What applications is Recombinant Human ING3 Protein (rp376578) validated for?
Recombinant Human ING3 Protein is suitable for protein-protein interaction studies, biochemical assays, antibody validation (e.g., as a positive control in Western blot or ELISA), and structural or biophysical characterization. Researchers should consult the current product datasheet for specific validated conditions and recommended working concentrations, as application-specific data may vary by lot.
How should Recombinant Human ING3 Protein be stored to maintain its stability?
As a general best practice for recombinant proteins, rp376578 should be stored at -20°C or -80°C upon receipt. To avoid activity loss caused by repeated freeze-thaw cycles, it is strongly recommended to prepare single-use aliquots before freezing. Always refer to the product-specific datasheet for confirmed storage conditions and shelf-life information.
What is the difference between ING3 and other ING family members?
The ING family comprises five members (ING1–ING5) in humans, all sharing a conserved PHD finger that binds H3K4me3. However, each member associates with distinct chromatin-modifying complexes: ING3 is uniquely associated with the NuA4/TIP60 HAT complex, whereas ING4 and ING5 associate with HBO1, and ING1 and ING2 associate with Sin3A HDAC complexes. These distinct complex associations confer different transcriptional and biological outcomes.
How can I confirm the purity and quality of my lot of rp376578?
Lot-specific quality data, including purity assessment by SDS-PAGE and relevant activity or functional data, are provided in the Certificate of Analysis (CoA) for each batch. You may request the CoA for your specific lot from Aladdin Scientific technical support or download it from the product page on the Aladdin Scientific website using your lot number.
References
- Doyon Y, Cayrou C, Ullah M, et al. ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. Molecular Cell. 2006;21(1):51-64. doi:10.1016/j.molcel.2005.12.007
- Shi X, Gozani O. The fellowships of the INGs. Journal of Cellular Biochemistry. 2005;96(6):1127-1136. doi:10.1002/jcb.20637
- Campos EI, Chin MY, Kuo WH, Li G. Biological functions of the ING family tumor suppressors. Cellular and Molecular Life Sciences. 2004;61(19-20):2597-2613. doi:10.1007/s00018-004-4199-4
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
危险品化学品经营许可证（带存储）