What Is KTI12 and What Does It Do?

KTI12 (Killer Toxin Insensitive 12) is a conserved eukaryotic protein originally identified in yeast as a factor involved in resistance to zymocin, a tRNA anticodon nuclease toxin. In humans, KTI12 is understood to function as a regulatory component associated with the Elongator complex, a multi-subunit protein assembly that plays key roles in tRNA wobble-position uridine modification, transcriptional elongation, and exocytosis.

By interacting with the Elongator complex, KTI12 is thought to modulate its acetyltransferase activity, thereby influencing the efficiency and fidelity of translation through proper tRNA modification. Dysregulation of Elongator-associated proteins, including KTI12, has been linked to neurological conditions and developmental disorders in model organisms, making KTI12 of interest in translational and neuroscience research.

Product Specifications and Quality

Recombinant Human KTI12 Protein (rp373183) is produced as a recombinant protein for research use. Specific details regarding the expression system (e.g., E. coli, baculovirus, mammalian), tag configuration, molecular weight, purity grade, and endotoxin levels are provided in the official product datasheet available on the Aladdin Scientific website. Researchers are strongly encouraged to download and review the current datasheet before use.

General best practices for recombinant proteins of this class include:

• Purity: Typically >85–95% by SDS-PAGE for research-grade recombinant proteins, though the exact specification should be confirmed in the datasheet.
• Formulation: Usually supplied in a buffered solution; refer to the datasheet for exact buffer composition and protein concentration.
• Endotoxin: Endotoxin levels are tested and reported; confirm acceptable thresholds for your specific assay.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human KTI12 Protein are critical to maintaining activity and integrity. While exact conditions are specified in the product datasheet, the following general guidelines apply to most recombinant proteins:

• Short-term storage: Store at 4 °C for up to 1–2 weeks if the protein will be used promptly.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Carrier protein: Addition of a stabilizing agent such as 0.1% BSA may be recommended for dilute preparations; consult the datasheet.
• Avoid: Repeated freeze-thaw cycles, exposure to detergents incompatible with the formulation, and prolonged storage at room temperature.

Always centrifuge the vial briefly upon receipt and after thawing to consolidate the contents before opening.

Recommended Research Applications

Recombinant Human KTI12 Protein is intended for in vitro research use only and is not approved for therapeutic, diagnostic, or veterinary applications. Typical research applications for recombinant proteins in the KTI12 class include:

• Protein–protein interaction studies: Co-immunoprecipitation (Co-IP) or pull-down assays to map KTI12 interactions with Elongator subunits or other binding partners.
• Biochemical activity assays: In vitro reconstitution of Elongator complex activity to assess KTI12's modulatory role.
• Structural studies: As a component for cryo-EM or X-ray crystallography investigations of Elongator complex architecture.
• Western blot controls: As a positive control antigen for antibody validation experiments.
• Cell-free translation studies: Investigating the impact of KTI12 on tRNA modification efficiency.

Always validate the protein's activity in your specific assay system using appropriate positive and negative controls.

Ordering, Shipping, and Returns

Recombinant Human KTI12 Protein (SKU: rp373183) can be ordered directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative.

• Ordering: Add the product to your cart on the Aladdin Scientific website, or submit a purchase order quoting SKU rp373183. Bulk or custom quantity inquiries are welcomed.
• Shipping: Recombinant proteins are temperature-sensitive and are shipped on dry ice or with cold packs as appropriate. Confirm cold-chain requirements with customer service prior to ordering if your location has specific import or customs requirements.
• Returns and refunds: For quality concerns, contact Aladdin Scientific customer service promptly upon receipt. Returns are subject to Aladdin Scientific's standard return and refund policy. Product must be reported within the specified window; see the company's returns policy page for details.
• Lead time: Standard lead times apply; consult the product page or customer service for current availability and estimated delivery.