What Is L3MBTL3 and Why Is It Important?

L3MBTL3 (Lethal-3 Malignant Brain Tumor-Like Protein 3) is a member of the MBT (malignant brain tumor) repeat protein family. These proteins are characterized by the presence of two to four tandem MBT repeats that function as methyl-lysine readers on histone tails. L3MBTL3 specifically recognizes mono- and di-methylated lysine residues on histones H3 and H4, contributing to chromatin compaction and transcriptional silencing.

Dysregulation of L3MBTL3 has been implicated in several cancers and developmental disorders, making it a valuable target for epigenetic research. As a recombinant protein, it enables biochemists and cell biologists to dissect its molecular interactions in controlled in vitro settings.

Product Specifications and Quality

The Recombinant Human L3MBTL3 Protein (SKU: rp367124) is produced using recombinant expression systems to ensure reproducibility and consistency across research applications. Key specification considerations include:

• Purity: Recombinant proteins from Aladdin Scientific are typically evaluated by SDS-PAGE; specific purity data should be confirmed in the product datasheet.
• Formulation: Supplied in a stabilizing buffer appropriate for protein storage; refer to the current product datasheet for exact formulation details.
• Biological Activity: Functional activity is typically confirmed via histone peptide binding assays or equivalent biochemical methods.

Always consult the most current Certificate of Analysis (CoA) and product datasheet at time of use to verify lot-specific specifications.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human L3MBTL3 Protein are essential to maintain protein integrity and biological activity:

• Storage Temperature: Store at −20 °C or −80 °C for long-term stability; avoid repeated freeze–thaw cycles.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze–thaw degradation.
• Carrier Protein: If the protein is supplied at low concentration, consider adding BSA as a carrier to improve stability.
• Working Conditions: Keep on ice during experimental setup. Do not expose to prolonged room temperature.

Specific storage conditions and shelf-life information are provided on the product datasheet and CoA. Contact Aladdin Scientific technical support if you observe unexpected activity loss.

Typical Research Applications

Recombinant Human L3MBTL3 Protein is used across a range of epigenetic and chromatin biology research workflows:

• Histone Peptide Binding Assays: Assess the affinity and specificity of L3MBTL3 MBT domains for methylated histone marks (e.g., H4K20me1, H3K4me1).
• Pull-Down and Co-Immunoprecipitation Studies: Identify novel interaction partners of L3MBTL3 within chromatin complexes.
• Structural Biology: Use as antigen or binding partner in crystallography or cryo-EM studies of MBT domain–histone complexes.
• Inhibitor Screening: Deploy as a target protein in competitive binding or displacement assays for small-molecule epigenetic inhibitor discovery.
• Biochemical Reconstitution: Reconstruct chromatin repressor complexes in vitro to study their mechanistic roles.

Ordering, Returns, and Technical Support

Recombinant Human L3MBTL3 Protein (SKU: rp367124) can be ordered directly through the Aladdin Scientific website or by contacting your regional sales representative.

• Ordering: Add SKU rp367124 to your cart or submit a purchase order. Bulk or custom quantity inquiries are welcome through the sales team.
• Shipping: Recombinant proteins are shipped on dry ice to maintain cold-chain integrity. Specific shipping conditions are confirmed at time of order.
• Returns and Refunds: If you receive a damaged or non-conforming product, contact Aladdin Scientific customer service within the specified return window. Please retain all original packaging and documentation.
• Technical Support: For application-specific questions, protocol guidance, or troubleshooting, contact the Aladdin Scientific technical support team. Reference SKU rp367124 and your lot number when inquiring.