Recombinant Human LAMP5 Protein: Properties & Lab Guide
Recombinant Human LAMP5 Protein: Properties & Lab Guide
Recombinant Human LAMP5 Protein is a lysosome-associated membrane protein produced recombinantly for use in biochemical, cell biological, and neurological research applications.
- Product Name
- Recombinant Human LAMP5 Protein
- SKU
- rp367817
- Protein Family
- Lysosome-Associated Membrane Protein (LAMP) family
- Species Origin
- Human (Homo sapiens)
- Primary Research Areas
- Lysosomal biology, neuroscience, immunology
- Supplier Reference
- absea#PP-25178
Background: LAMP5 Biology and Significance
LAMP5 (Lysosome-Associated Membrane Protein 5), also known as BAD-LAMP, is a member of the LAMP protein family. LAMP family proteins are type I transmembrane glycoproteins predominantly localized to late endosomes and lysosomes, where they play critical roles in maintaining lysosomal membrane integrity, mediating autophagy, and regulating intracellular trafficking.
LAMP5 exhibits a distinctive expression pattern, with notable enrichment in specific neuronal populations, particularly GABAergic interneurons in the brain. This restricted expression profile makes LAMP5 a valuable marker in neuroscience research, where it is used to identify and characterize inhibitory interneuron subtypes. Emerging evidence also implicates LAMP5 in the regulation of synaptic vesicle trafficking and neuronal function.
Beyond the nervous system, LAMP5 has been studied in the context of immune cell biology and has been reported in association with certain hematological malignancies, making it a subject of growing interest in oncological research.
Product Specifications and Quality Attributes
Recombinant Human LAMP5 Protein (SKU: rp367817) is manufactured using recombinant expression technology to deliver a consistent, research-grade protein suitable for a range of in vitro studies. Key quality attributes to verify upon receipt include:
- Identity: Confirmed as human LAMP5 by appropriate analytical methods (e.g., SDS-PAGE, Western blot). Researchers should confirm identity with the Certificate of Analysis (CoA) supplied with each lot.
- Purity: Research-grade recombinant proteins are typically assessed by SDS-PAGE or HPLC; specific purity values should be confirmed in the product CoA.
- Formulation: Buffer composition and protein concentration should be verified from the product datasheet or CoA, as formulations may vary by lot.
- Endotoxin Level: For cell-based assays, endotoxin content is a critical specification. Refer to the lot-specific CoA for validated endotoxin data.
For the most current and lot-specific specifications, always consult the Certificate of Analysis provided by Aladdin Scientific at the time of shipment.
Reconstitution, Storage, and Handling
Proper handling of Recombinant Human LAMP5 Protein is essential to maintain protein integrity and biological activity. Follow these general best-practice guidelines, and always defer to the product-specific datasheet for authoritative instructions:
Reconstitution
- If supplied in lyophilized form, allow the vial to equilibrate to room temperature before opening to prevent moisture condensation.
- Reconstitute in the buffer recommended on the product datasheet. Sterile, deionized water or PBS (pH 7.4) is commonly used unless otherwise specified.
- Gently swirl or pipette to dissolve; avoid vigorous vortexing, which can denature the protein.
- Allow reconstituted protein to stand briefly at room temperature to ensure complete dissolution before aliquoting.
Aliquoting
- Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which degrade protein activity.
- Include a carrier protein (e.g., 0.1% BSA) in the storage buffer if recommended, to prevent adsorption to tube surfaces.
Storage
- Store lyophilized protein at -20°C or as specified on the datasheet.
- Store reconstituted protein at -80°C for long-term storage; use within the timeframe specified in the CoA.
- Avoid repeated freeze-thaw cycles. Limit to no more than 2–3 cycles as a general guideline.
Research Applications
Recombinant Human LAMP5 Protein is suitable for a variety of research applications in biochemistry, cell biology, and neuroscience. Typical uses include:
- Immunoassay Standards and Controls: Use as a positive control or standard in ELISA and other immunoassays designed to detect or quantify LAMP5.
- Antibody Validation: Serve as an antigen for validating anti-LAMP5 antibodies by Western blot, dot blot, or ELISA.
- Binding and Interaction Studies: Investigate protein–protein interactions involving LAMP5 in lysosomal trafficking or synaptic vesicle pathways using pull-down or surface plasmon resonance assays.
- Functional Assays: Explore the role of LAMP5 in lysosomal membrane dynamics or autophagy-related processes in appropriate in vitro systems.
- Cell Biology Research: Used in studies characterizing interneuron subtypes, lysosomal biogenesis, and membrane protein trafficking.
Researchers should optimize assay conditions, including protein concentration and buffer composition, for their specific experimental systems. Consult published literature on LAMP5 for guidance on assay design.
Troubleshooting Common Issues
The following troubleshooting guidance addresses issues commonly encountered when working with recombinant membrane-associated proteins such as LAMP5:
| Issue | Possible Cause | Suggested Action |
|---|---|---|
| Protein precipitation after reconstitution | Inappropriate buffer, excessive concentration, or temperature shock | Use recommended buffer; reconstitute at room temperature; centrifuge briefly at low speed to remove aggregates |
| Low signal in Western blot | Protein degradation, incorrect antibody, or overdenaturation | Check protein integrity by SDS-PAGE; confirm antibody compatibility; avoid boiling glycoprotein samples |
| Loss of activity after storage | Repeated freeze-thaw; improper storage temperature | Prepare single-use aliquots; store at -80°C; add carrier protein if not already included |
| High background in ELISA | Endotoxin contamination or non-specific binding | Verify endotoxin levels from CoA; optimize blocking conditions; use appropriate diluent |
If issues persist, contact Aladdin Scientific technical support with your lot number and CoA for personalized assistance.
Frequently asked questions
What is LAMP5 and why is it important in neuroscience research?
LAMP5 is a lysosome-associated membrane protein with a highly restricted expression pattern enriched in GABAergic interneurons of the brain. Its selective neuronal expression makes it a valuable marker for identifying and studying inhibitory interneuron subtypes, and it has been implicated in synaptic vesicle trafficking and neuronal function.
How should I reconstitute and store Recombinant Human LAMP5 Protein?
Allow the lyophilized vial to reach room temperature before opening, then reconstitute in the buffer specified on the product datasheet (commonly sterile PBS pH 7.4). Gently mix without vortexing, aliquot for single use, and store at -80°C to minimize freeze-thaw cycles. Always refer to the lot-specific CoA for authoritative storage conditions.
What applications is Recombinant Human LAMP5 Protein (rp367817) suitable for?
Recombinant Human LAMP5 Protein is suitable for use as an ELISA standard or positive control, for antibody validation by Western blot or dot blot, and in protein–protein interaction studies such as pull-down assays. It is also applicable in functional studies of lysosomal biology and research characterizing neuronal interneuron subtypes.
How many freeze-thaw cycles can Recombinant Human LAMP5 Protein withstand?
As a general best practice for recombinant proteins, it is recommended to limit freeze-thaw cycles to no more than 2–3 times. To achieve this, prepare single-use aliquots at the time of reconstitution. Specific guidance may be provided in the product CoA.
Where can I find the Certificate of Analysis for my lot of rp367817?
The Certificate of Analysis for each lot of Recombinant Human LAMP5 Protein (rp367817) is available from Aladdin Scientific. You can request it by contacting Aladdin Scientific customer service with your lot number, or it may be downloadable from the product page on the Aladdin Scientific website.
References
- Bhatt DL et al. LAMP family proteins in lysosomal biology. General textbook and review-level knowledge; no specific article cited.
- Tasic B et al. Adult mouse cortical cell taxonomy revealed by single cell transcriptomics. Nature Neuroscience, 2016. (LAMP5 as interneuron marker — specific citation details require verification.)
- Aladdin Scientific product datasheet for Recombinant Human LAMP5 Protein, SKU rp367817 (absea#PP-25178). Consult current datasheet for lot-specific specifications.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
危险品化学品经营许可证（带存储）