Background: LANCL1 Protein Biology

LANCL1 (LanC-like protein 1) is a mammalian homolog of bacterial LanC enzymes, originally identified in the context of lanthionine synthesis pathways. In humans, LANCL1 is ubiquitously expressed and has been implicated in G-protein-coupled signaling, cellular energy metabolism, and immune modulation. The protein localizes predominantly to the cytoplasm and plasma membrane, where it is proposed to interact with G-protein subunits and participate in downstream signaling cascades.

Research into LANCL1 has expanded to include its role as a putative receptor or co-receptor for abscisic acid (ABA) and its involvement in insulin signaling pathways. These functions make LANCL1 a protein of interest in the study of metabolic disorders, neurological conditions, and immune regulation. Recombinant Human LANCL1 Protein (rp369002) provides a well-defined tool for investigating these mechanisms in vitro.

Reconstitution Protocol

Follow the steps below to reconstitute Recombinant Human LANCL1 Protein (rp369002) prior to use:

1. Equilibrate to room temperature: Allow the vial of lyophilized protein to equilibrate to room temperature (18–25 °C) for 10–15 minutes before opening, to minimize moisture condensation.
2. Prepare reconstitution buffer: Unless otherwise specified on the Certificate of Analysis (CoA), reconstitute in sterile, deionized water or an appropriate buffer (e.g., PBS, pH 7.4) to yield a stock concentration typically between 0.1–1.0 mg/mL. Consult the product CoA for the recommended volume.
3. Gentle mixing: Gently swirl or pipette the solution up and down. Do NOT vortex vigorously, as this may cause protein denaturation or aggregation.
4. Centrifuge briefly: Spin the vial at 500–1000 × g for 1 minute to collect any residual material from the walls of the vial.
5. Aliquot and store: Prepare single-use aliquots to avoid repeated freeze-thaw cycles. Store working aliquots at -80 °C for long-term storage or at 4 °C for short-term use (up to 1 week). Add a carrier protein (e.g., 0.1% BSA) if diluting to very low concentrations to minimize non-specific adsorption.

Note: Always refer to the product-specific Certificate of Analysis (CoA) for lot-specific reconstitution instructions, as conditions may vary.

Experimental Application Guidelines

Recombinant Human LANCL1 Protein (rp369002) is suitable for a range of in vitro research applications. Recommended uses include:

• Protein–Protein Interaction Studies: Use LANCL1 protein as bait or prey in pull-down assays, co-immunoprecipitation validation, or surface plasmon resonance (SPR) experiments to map binding partners.
• Enzyme Activity Assays: Investigate potential enzymatic functions of LANCL1 in biochemical assay formats relevant to the LanC enzyme family.
• Antibody Validation: Use as a positive control antigen for western blot, ELISA, or immunofluorescence assays targeting LANCL1.
• Cell-Free Signaling Assays: Incorporate into cell-free systems to study LANCL1 interactions with G-protein subunits or other signaling components.
• Structural Studies: Suitable as a substrate for crystallography or other structural biology workflows, subject to purity and buffer compatibility requirements.

Optimal working concentrations must be determined empirically for each application. Always include appropriate positive and negative controls in every experiment.

Quality Control and Handling

Aladdin Scientific's Recombinant Human LANCL1 Protein (rp369002) undergoes standard quality control testing prior to release. Researchers should observe the following handling best practices:

• Purity verification: Confirm purity by SDS-PAGE upon receipt, comparing against the Certificate of Analysis. Expected purity is typically ≥85% (consult CoA for lot-specific data).
• Sterility: Handle under aseptic conditions when using in cell-based assays. Use sterile-filtered buffers and work in a laminar flow hood if sterility is required.
• Endotoxin levels: For applications sensitive to endotoxin contamination (e.g., cell culture, in vivo studies), verify endotoxin levels against the CoA specification before use.
• Avoid contamination: Use fresh pipette tips for each aliquot. Do not return unused protein to the original vial to prevent cross-contamination.
• Stability: Lyophilized protein is stable for up to 12 months at -20 °C when stored properly. Reconstituted protein should be used within the timeframe specified on the CoA.

Ordering and Support

Recombinant Human LANCL1 Protein is available from Aladdin Scientific under SKU rp369002. To place an order or request additional technical documentation, including the full Certificate of Analysis, Safety Data Sheet (SDS), or application-specific support, please contact your Aladdin Scientific representative or visit the product page on the Aladdin Scientific website.

For bulk orders, custom formulations, or questions about protein specifications, purity grades, or compatibility with your specific experimental workflow, our technical support team is available to assist. All products are intended for research use only and are not suitable for diagnostic or therapeutic applications in humans.