What Is LANCL2 and Why Is It Studied?

LANCL2 (LanC-like protein 2) is a member of the mammalian LANCL protein family, which are structural and sequence homologs of bacterial LanC enzymes involved in lantibiotic biosynthesis. In mammals, LANCL2 is widely expressed and has been implicated in a range of biological processes including immune cell function, insulin signaling, and energy metabolism.

A key area of research interest is the reported role of LANCL2 as a receptor or co-receptor for abscisic acid (ABA), a plant hormone that also occurs in mammalian tissues at low concentrations. Through this axis, LANCL2 has been linked to the modulation of inflammatory pathways and the activation of immune cells such as granulocytes and dendritic cells. These properties make LANCL2 an attractive target for researchers studying immune modulation, metabolic disease, and inflammatory conditions.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human LANCL2 Protein (SKU: rp369018) is produced as a recombinant protein intended for research applications. As with all recombinant proteins in Aladdin's catalog, researchers should consult the official product datasheet for the most current and authoritative specifications, including:

• Molecular weight: Confirm from datasheet, as tags or truncations may affect apparent MW on SDS-PAGE.
• Purity: Typically assessed by SDS-PAGE and/or HPLC; consult the datasheet for the lot-specific certificate of analysis (CoA).
• Formulation: Buffer composition and additives are specified per lot on the CoA.
• Endotoxin level: Critical for cell-based and in vivo assays; confirm from CoA.

For questions about specific lot data, contact Aladdin Scientific technical support or request the CoA at the time of ordering.

Storage, Handling, and Stability

Proper storage and handling are essential to maintain the activity and integrity of recombinant proteins. The following are general best-practice guidelines for recombinant proteins such as LANCL2; always verify against the product-specific datasheet:

• Short-term storage (up to 1 month): Store at 4 °C if the protein will be used within a short period after receipt.
• Long-term storage: Aliquot and store at −80 °C to prevent repeated freeze-thaw cycles, which can cause aggregation and loss of activity.
• Avoid repeated freeze-thaw: Prepare single-use aliquots upon first thaw.
• Carrier protein: For dilute solutions, the addition of a carrier protein (e.g., 0.1% BSA) may improve stability.
• Working concentration: Reconstitute or dilute in the buffer recommended on the datasheet.

If you observe unexpected aggregation, precipitation, or loss of activity, contact Aladdin Scientific technical support for troubleshooting assistance.

Common Research Applications

Recombinant Human LANCL2 Protein is suitable for a range of in vitro research applications. Typical uses include but may not be limited to:

• Protein–protein interaction studies: Identifying binding partners of LANCL2 using pull-down assays, co-immunoprecipitation, or surface plasmon resonance (SPR).
• Biochemical assays: Enzyme activity assays if enzymatic function is being investigated; structural studies by techniques such as X-ray crystallography or cryo-EM.
• Cell-based functional assays: Investigating the role of exogenous LANCL2 in cellular signaling cascades, particularly in relation to ABA-dependent pathways.
• Antibody validation: Using purified recombinant protein as a positive control or immunogen for antibody development and validation.
• Drug discovery: As a target protein for screening small-molecule modulators of the LANCL2–ABA signaling axis.

Researchers should optimize assay conditions including protein concentration, buffer, and incubation parameters for their specific experimental system.

Ordering, Shipping, and Technical Support

Recombinant Human LANCL2 Protein (SKU: rp369018) can be ordered directly through the Aladdin Scientific website or by contacting your regional Aladdin representative. Key points for procurement:

• Availability: Check the product page for current stock status and lead times.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Confirm shipping conditions with Aladdin Scientific at the time of order.
• Returns and quality issues: If the product does not meet specifications upon receipt, contact Aladdin Scientific customer service promptly with your order number and CoA for assistance in accordance with the return and refund policy.
• Technical support: For application-specific guidance, protocol optimization, or troubleshooting, contact the Aladdin Scientific technical support team via the website or by phone.
• Bulk and custom requests: Contact Aladdin Scientific for pricing and availability of custom formulations or bulk quantities.