Background: LANCL2 Biology and Significance

LANCL2 (LanC-like protein 2) is a mammalian homolog of the bacterial LanC enzyme superfamily, originally identified for its structural resemblance to prokaryotic lantibiotic synthetase enzymes. In humans, LANCL2 is broadly expressed across multiple tissues including the brain, immune cells, and adipose tissue, suggesting pleiotropic physiological roles.

Emerging research indicates that LANCL2 functions as a receptor or co-receptor involved in immune modulation and metabolic homeostasis. It has been proposed to interact with abscisic acid (ABA) and lanthionine-related metabolites, linking it to anti-inflammatory signaling cascades. Activation of LANCL2 pathways has been associated with the regulation of innate and adaptive immune responses, insulin sensitivity, and inflammatory resolution, making it a target of interest in autoimmune disease, metabolic syndrome, and inflammatory bowel disease research.

Recombinant Human LANCL2 Protein enables researchers to study these interactions in controlled in vitro environments, supporting mechanistic investigations into LANCL2-mediated signaling without the complexity of whole-cell or in vivo systems.

Protein Specifications and Quality Attributes

Recombinant Human LANCL2 Protein (rp369019) is produced using recombinant expression systems to ensure consistent batch-to-batch quality suitable for demanding research applications. Key quality attributes researchers should review on the official product datasheet include:

• Purity: Determined by SDS-PAGE and/or HPLC; specific purity values are indicated on the product datasheet and certificate of analysis (CoA).
• Formulation: The protein is supplied in a defined buffer system optimized for stability; refer to the current lot-specific CoA for exact formulation details.
• Molecular Weight: Confirmed by reducing SDS-PAGE; apparent molecular weight may differ from calculated weight due to post-translational modifications or tags.
• Endotoxin Level: Tested by LAL or equivalent assay; low endotoxin specification ensures suitability for cell-based and functional assays.
• Biological Activity: Where applicable, functional activity is verified by binding or enzymatic assay as described in the product datasheet.

Always consult the lot-specific CoA supplied with each shipment for precise numerical values, as specifications may be updated.

Reconstitution and Handling Protocol

Proper handling of Recombinant Human LANCL2 Protein is essential to maintain its biological activity and structural integrity. Follow these general guidelines, and always defer to the instructions provided in the product-specific datasheet:

1. Reconstitution: If supplied lyophilized, briefly centrifuge the vial before opening. Reconstitute in sterile, deionized water or the buffer specified in the datasheet to the recommended concentration. Allow the protein to dissolve gently; do not vortex.
2. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which can degrade protein activity.
3. Storage: Store lyophilized protein at -20°C or -80°C as directed. Reconstituted protein should be stored at -80°C for long-term use or at 4°C for short-term use (typically ≤1 week). Add a carrier protein such as 0.1% BSA if long-term stability is a concern.
4. Avoid Repeated Freeze-Thaw: Each freeze-thaw cycle may reduce activity. Use single-use aliquots to preserve integrity.
5. Working Concentration: Optimize the working concentration empirically for each assay type. Typical ranges for binding assays are provided in the datasheet where available.

Wear appropriate personal protective equipment (PPE) when handling. Consult the Safety Data Sheet (SDS) prior to use.

Research Applications of LANCL2 Protein

Recombinant Human LANCL2 Protein is suited to a range of in vitro research applications where a pure, well-characterized human protein source is required:

• Protein–Ligand Binding Assays: Use to investigate binding interactions between LANCL2 and candidate ligands such as abscisic acid derivatives or lanthionine metabolites in surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), or ELISA-based formats.
• Structural Biology: Suitable as an antigen for antibody generation or as a standard in Western blot validation experiments.
• Signaling Pathway Studies: Supports investigation of LANCL2-mediated downstream signaling, including cAMP pathway activation and immune cell functional assays.
• Drug Discovery: Can be used in high-throughput screening (HTS) platforms to identify small-molecule modulators of LANCL2 activity relevant to inflammation, diabetes, or autoimmune disease.
• Positive Control: Serves as a reliable positive control in immunoassays detecting endogenous LANCL2 expression.

Researchers should validate the protein in their specific assay systems, as performance may vary by experimental conditions.

Quality Control and Ordering Information

Aladdin Scientific's Recombinant Human LANCL2 Protein (SKU: rp369019) undergoes rigorous quality control testing prior to release. Each lot is accompanied by a Certificate of Analysis (CoA) documenting test results for purity, identity, and where applicable, biological activity.

How to Order: Contact Aladdin Scientific through the website or authorized distributors. Reference SKU rp369019 when placing your order to ensure correct product selection. Custom quantities or bulk orders may be available upon request.

Technical Support: For protocol optimization, application-specific questions, or datasheet clarification, contact the Aladdin Scientific technical support team. Provide your lot number (found on product label and CoA) for the most accurate assistance.

Related Resources: Aladdin Scientific provides Safety Data Sheets (SDS) and product datasheets available for download on the product page. Review these documents before initiating experiments.