Background: LAPTM4B Biology and Significance

LAPTM4B (Lysosomal Protein Transmembrane 4 Beta) is a member of the LAPTM protein family, which are integral membrane proteins predominantly localized to lysosomes and late endosomes. The human LAPTM4B gene encodes a multi-pass transmembrane protein involved in the regulation of lysosomal function, cellular drug resistance, autophagy, and vesicular trafficking.

LAPTM4B has attracted considerable attention in cancer biology, where it has been identified as overexpressed in multiple tumor types, potentially contributing to tumor progression, chemotherapy resistance, and poor clinical prognosis. As such, it represents a subject of active investigation as both a potential biomarker and a therapeutic target.

Recombinant Human LAPTM4B Protein enables researchers to study these mechanisms in a controlled, reproducible manner outside the complexity of intact cellular systems, supporting functional studies, antibody validation, and assay development.

Reconstitution and Handling Guidelines

Proper reconstitution and handling of Recombinant Human LAPTM4B Protein are essential to maintain protein integrity and biological activity. Follow the specific instructions provided on the product datasheet (SKU: rp368856) for exact buffer compositions and volumes.

• Centrifugation: Before opening the vial, briefly centrifuge to consolidate lyophilized material at the bottom of the tube and minimize loss during opening.
• Reconstitution buffer: Reconstitute using the buffer specified on the product datasheet. Generally, sterile phosphate-buffered saline (PBS) or a manufacturer-recommended buffer containing a carrier protein (e.g., 0.1% BSA) may be used. Add buffer gently to the side of the vial to avoid foaming.
• Mixing: Gently swirl or pipette to mix. Do not vortex, as vigorous agitation can denature the protein and reduce activity.
• Equilibration: Allow the reconstituted protein to equilibrate at the recommended temperature before use.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to prevent activity loss from repeated freeze-thaw cycles.

Always wear appropriate personal protective equipment (PPE) when handling recombinant proteins. Consult the Safety Data Sheet (SDS) for detailed safety information.

Storage Conditions and Stability

Maintaining correct storage conditions is critical to preserving the quality and activity of Recombinant Human LAPTM4B Protein throughout its shelf life. Refer to the specific product datasheet for authoritative guidance.

• Lyophilized form: Store lyophilized protein at -20°C or -80°C as indicated. Keep desiccated and protect from moisture. Lyophilized proteins are generally more stable and may be stored for longer periods when kept dry and cold.
• Reconstituted form: Once reconstituted, store at -80°C for long-term preservation. For short-term use (within days), storage at 4°C may be acceptable if indicated by the datasheet, but this should be verified on the specific product documentation.
• Freeze-thaw cycles: Minimize freeze-thaw cycles. Each cycle can reduce protein activity and promote aggregation. Prepare and store single-use aliquots.
• Avoid: Prolonged exposure to room temperature, repeated freeze-thaw, and storage at 4°C for extended periods without specific guidance.

If unexpected precipitation or turbidity is observed, consult Aladdin Scientific technical support before proceeding with experiments.

Experimental Applications

Recombinant Human LAPTM4B Protein is suitable for a range of research applications. Scientists should validate the protein for each specific assay format in their laboratory.

• Antibody validation and epitope mapping: Use as a positive control antigen to confirm antibody specificity in Western blotting, ELISA, and immunoprecipitation workflows.
• Biochemical interaction studies: Employ in pull-down assays or surface plasmon resonance (SPR) to characterize protein-protein interactions involving LAPTM4B.
• Functional and enzymatic assays: Assess the role of LAPTM4B in lysosomal function, drug transport, or autophagy regulation in cell-free or reconstituted systems.
• Assay development and standard curve generation: Use as a reference standard in quantitative assays such as sandwich ELISA for LAPTM4B detection in biological samples.
• Cancer research: Investigate LAPTM4B's contribution to multidrug resistance, tumor cell survival, and tumor biomarker discovery.

Always optimize protein concentration, buffer conditions, and incubation times empirically for each application. Performance may vary by experimental format and sample matrix.

Quality, Ordering, and Technical Support

Aladdin Scientific is committed to providing high-quality recombinant proteins for life science research. Recombinant Human LAPTM4B Protein (SKU: rp368856) is manufactured and quality-controlled to support reproducible research outcomes.

• Purity and QC: Each lot is subject to quality control testing. Refer to the Certificate of Analysis (CoA) provided with the product for lot-specific purity, concentration, and activity data.
• Ordering: To place an order, visit the Aladdin Scientific website and search for SKU rp368856, or contact your regional Aladdin Scientific representative. Bulk quantities and custom formulations may be available upon request.
• Technical support: For application-specific guidance, reconstitution assistance, or troubleshooting, contact Aladdin Scientific technical support. Provide the product SKU, lot number, and a description of the experimental issue to expedite assistance.
• Returns and replacements: For quality concerns, refer to Aladdin Scientific's return and refund policy or contact customer service directly.

Always retain the product datasheet, CoA, and SDS for your records and institutional compliance requirements.