Biological Background of LARP4

LARP4 (La-Related Protein 4) is a member of the conserved La-related protein (LARP) superfamily, a group of RNA-binding proteins defined by the presence of a La motif (LaM) and an adjacent RNA recognition motif (RRM). These structural domains enable LARPs to interact selectively with RNA substrates, coordinating post-transcriptional gene regulation.

LARP4 is ubiquitously expressed in human tissues and has been implicated in the stabilization of poly(A)-tailed mRNAs, associating with poly(A)-binding proteins (PABPs) to protect transcripts from degradation. Through these interactions, LARP4 contributes to the regulation of protein synthesis rates and cellular homeostasis. Research into LARP4 has expanded interest in understanding how RNA-binding proteins collectively orchestrate translational output in response to physiological and pathological signals.

Biochemical Properties of Recombinant Human LARP4 Protein

Recombinant Human LARP4 Protein is produced using recombinant expression systems to deliver a well-defined, research-grade protein suitable for a range of in vitro applications. Recombinant production ensures batch-to-batch consistency in terms of sequence integrity and functional activity compared to protein extracted directly from human cell lysates.

The recombinant protein retains the functional La motif and RNA recognition motif characteristic of native LARP4, supporting its use in biochemical binding assays and structural studies. Researchers should consult the specific product datasheet (SKU: rp372965) for confirmed specifications including molecular weight, tag information, purity grade, formulation buffer, and recommended storage conditions, as these parameters are critical for experimental reproducibility.

Research Applications

Recombinant Human LARP4 Protein is suited for a variety of research applications in the fields of RNA biology and post-transcriptional regulation:

• RNA-Binding Assays: LARP4 can be used in electrophoretic mobility shift assays (EMSA), RNA immunoprecipitation (RIP), and in vitro pull-down experiments to characterize its interactions with poly(A) RNA and specific mRNA targets.
• Protein–Protein Interaction Studies: Co-immunoprecipitation and surface plasmon resonance (SPR) assays can be employed to investigate LARP4 interactions with poly(A)-binding proteins (PABPs) and other components of the mRNP complex.
• mRNA Stability Assays: Recombinant LARP4 can be incorporated into cell-free systems to assess its effect on mRNA half-life and poly(A) tail dynamics.
• Structural Biology: The purified recombinant protein is a suitable starting material for X-ray crystallography or cryo-electron microscopy studies aimed at resolving LARP4 domain architecture.
• Antibody Development and Validation: Recombinant LARP4 serves as a standard antigen for raising and validating anti-LARP4 antibodies used in Western blot, ELISA, and immunofluorescence applications.

Handling, Storage, and Quality Considerations

Proper handling and storage of recombinant proteins are essential for maintaining activity and ensuring reliable experimental results. As a general guideline for recombinant proteins of this class:

• Avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity. Aliquot the protein upon first use.
• Store according to the conditions specified on the product datasheet (SKU: rp372965). Recombinant proteins are typically stored at −20 °C or −80 °C in the supplied buffer.
• Centrifuge briefly before opening vials to collect any material from the cap or walls.
• Use low-binding tubes when working at low protein concentrations to minimize adsorption losses.

Researchers should validate the protein's activity and purity for their specific assay format using appropriate positive controls. For exact purity specifications, formulation details, and quality control data, consult the official Aladdin Scientific product datasheet.