What Is LARP4B and Why Is It Studied?

LARP4B (La-related protein 4B) is a member of the La-related protein (LARP) superfamily, which is characterized by the presence of a La motif (LaM) and an RNA recognition motif (RRM). LARP4B belongs to the LARP4 subfamily and is expressed in a wide range of human tissues. It functions primarily as an RNA-binding protein, associating with the poly(A) tails of mRNAs to promote their stability and facilitate translation. Research interest in LARP4B has grown due to its emerging roles in post-transcriptional gene regulation, cell proliferation, and its potential connections to cancer biology, where dysregulation of mRNA stability pathways can influence tumor progression.

As a recombinant protein, LARP4B is valuable for in vitro biochemical assays, protein–RNA interaction studies, structural investigations, and as a reference standard in assay development.

Product Specifications and Quality

Recombinant Human LARP4B Protein (SKU: rp372966) is manufactured as a recombinant protein. Key specification details such as exact molecular weight, host expression system (e.g., E. coli, baculovirus, or mammalian), purity grade, tag information, endotoxin levels, and formulation buffer are referenced under supplier catalog number absea #PP-6915. Researchers are advised to consult the current product datasheet available on the Aladdin Scientific website or by contacting customer support for the most up-to-date and verified specification values.

General quality standards for recombinant proteins of this class typically include SDS-PAGE or HPLC-verified purity, functional validation by binding assay or activity assay, and endotoxin testing by LAL method. Lot-specific certificates of analysis (CoA) are available upon request.

Recommended Applications and Handling

Recombinant Human LARP4B Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–RNA interaction studies: Electrophoretic mobility shift assays (EMSA) and RNA pull-down assays to characterize LARP4B binding to target mRNA sequences.
• Biochemical activity assays: Investigating the role of LARP4B in mRNA stabilization or translational efficiency in cell-free systems.
• Antibody validation: Use as a positive control antigen for ELISA, Western blot, or immunoprecipitation assay development.
• Structural studies: Crystallography or biophysical characterization of the La motif or RRM domains.

For handling, it is recommended to thaw the protein on ice, prepare single-use aliquots to avoid repeated freeze-thaw cycles, and use protein-low-binding tubes to minimize adsorptive losses. Reconstitution buffer and working concentration should follow the datasheet guidance.

Storage, Stability, and Shipping

Recombinant proteins, including LARP4B, are sensitive to improper storage conditions. The following general guidelines apply:

• Long-term storage: -80°C is recommended for extended storage periods to preserve protein integrity and biological activity.
• Short-term storage: -20°C is acceptable for storage up to several weeks, depending on formulation.
• Avoid repeated freeze-thaw cycles: Each freeze-thaw cycle can degrade protein activity. Prepare working aliquots at the time of first use.
• Shipping: Products are typically shipped on dry ice. Upon receipt, immediately transfer to appropriate storage temperature and inspect for integrity.

Specific stability data, including activity retention timelines, are provided in the lot-specific CoA or product datasheet. Contact Aladdin Scientific customer support for stability data specific to SKU rp372966.

Ordering, Pricing, and Support

Recombinant Human LARP4B Protein is available through Aladdin Scientific under SKU rp372966. To place an order or request a quotation, customers may:

• Visit the Aladdin Scientific website and search by SKU or product name.
• Contact the Aladdin Scientific sales team directly via email or phone for bulk pricing, custom formulations, or institutional purchasing agreements.
• Submit a purchase order through your institution's approved procurement channels.

For technical questions regarding experimental design, protein compatibility, or application-specific guidance, the Aladdin Scientific technical support team is available to assist. Certificates of Analysis (CoA) and Safety Data Sheets (SDS) are available for download on the product page or by request.